[10] Cystathionine

Reed, Donald J.

doi:10.1016/0076-6879(95)52012-0

Cited by 22 publications

(18 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All these findings suggested that both drugs are cell membrane permeable drugs and potent H 2 S-producing enzyme inhibitors. PAG is an irreversible inhibitor of CSE that crosses biological membrane readily (Reed, 1995;Stipanuk and Beck, 1982;Uren et al, 1978), whereas BCA causes reversible inhibition of CSE activity (Pfeffer and Ressler, 1967;Uren et al, 1978). However, in the present study, we found BCA produced reversible action after washout for 20 min only in very few cells.…”

Section: Discussioncontrasting

confidence: 85%

Hydrogen sulphide regulates calcium homeostasis in microglial cells

Lee¹,

Hu²,

Hu³

et al. 2006

Glia

135

View full text Add to dashboard Cite

Hydrogen sulphide (H 2 S), which is produced endogenously from L-cysteine in mammalian tissues, has been suggested to function as a neuromodulator in the brain. However, the role of H 2 S in microglial cells is unclear. In this study, the effect of exogenous and endogenous H 2 S on intracellular calcium homeostasis was investigated in primary cultured microglial cells. Sodium hydrosulphide (NaHS), a H 2 S donor, caused a concentration-dependent (0.1-0.5 mM) increase in intracellular calcium concentration ([Ca 21 ] i ). This effect was significantly attenuated in the presence of a calcium-free extracellular solution, Gd 31 (100 lM), a nonselective Ca 21 channel blocker, or thapsigargin (2 lM), an inhibitor of the sarcoplasmic/endoplasmic reticulum Ca 21 -ATPase. These observations suggest that the increase in [Ca 21 ] i in response to H 2 S involves both calcium influx across the plasma membrane and calcium release from intracellular stores. The H 2 S-induced calcium elevation is partly attenuated by H-89, a selective cAMP-dependent protein kinase (PKA) inhibitor, but not by U73122, a phospholipase C (PLC) inhibitor, and chelerythrine, a selective protein kinase C (PKC) inhibitor, suggesting the involvement of cAMP/PKA, but not PLC/PKC/phosphoinositol-3,4,5-inositol (IP 3 ) pathway. Using RT-PCR, only cystathionine g-lyase (CSE), a H 2 S producing enzyme, was detected in primary cultures of microglia. Lowering endogenous H 2 S level with, D,L-propargylglycine and b-cyano-L-alanine, two CSE inhibitors, significantly decreased [Ca 21 ] i , suggesting that endogenous H 2 S may have a positive tonic influence on [Ca 21 ] i homeostasis. These findings support the possibility that H 2 S may serve as a neuromodulator to facilitate signaling between neurons and microglial cells.

show abstract

Section: Discussioncontrasting

confidence: 85%

Hydrogen sulphide regulates calcium homeostasis in microglial cells

Lee¹,

Hu²,

Hu³

et al. 2006

Glia

135

View full text Add to dashboard Cite

show abstract

“…PAG is an irreversible inhibitor of CSE, and when administered to rodents, it produces an almost complete inhibition of liver CSE enzyme activity (Uren et al ., 1978). PAG is well absorbed and readily crosses biological membranes (Reed, 1995). We conclude therefore that PAG exhibits anti‐inflammatory activity in this model by virtue of inhibiting hindpaw CSE activity and thus H 2 S biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen sulphide is a mediator of carrageenan‐induced hindpaw oedema in the rat

Bhatia

Sidhapuriwala

Moochhala

et al. 2005

British J Pharmacology

146

104

View full text Add to dashboard Cite

Hydrogen sulphide (H 2 S) is a naturally occurring gas, with potent vasodilator activity. In this report, we identify a role for H 2 S in carrageenan-induced hindpaw oedema in the rat. Intraplantar injection of carrageenan (150 ml, 2% (w v À1 )) resulted in an increase in hindpaw H 2 S synthesising enzyme activity and increased myeloperoxidase (MPO) activity. Pretreatment (i.p. 60 min before carrageenan) with DL-propargylglycine (PAG, 25-75 mg kg À1 ), an inhibitor of the H 2 S synthesising enzyme cystathionine-g-lyase (CSE), significantly reduced carrageenan-induced hindpaw oedema in a dose-dependent manner (e.g. increase in hindpaw weight at 3 h, saline: 0.1270.017 g; carrageenan, 1.3970.037 g; PAG, 50 mg kg

show abstract

“…It has previously been shown that the complete and irreversible inactivation of cystathionine-␥ -lyase activity is achieved in vitro with DL -propargylglycine [30] . When administered to rodents, DL -propargylglycine produces an almost complete inhibition of liver cystathionine-␥ -lyase enzyme activity [31] ; it is well-absorbed and readily crosses biological membranes [32] . The conversion of L -cysteine to H 2 S in pancreas homogenates was significantly reduced in mice pretreated with DL -propargylglycine.…”

Section: Discussionmentioning

confidence: 99%