109 TUMOR NECROSIS FACTOR alpha(TNF-alpha) PRODUCTION BY MONOCYTES FROM CHILDREN WITH JUVENILE RHEUMATOID ARTHRITIS (JRA)

Abstract

The Netherlands (spn. by Margot van dc Uor) Red blood cell (RUC) peroxide catabolism, vla the synery,~sllc action of catalase and the glutatlllone rccycllng sy:jtesl (glutathione peroxidase and reductase), helps protect Lhe lung against oxygen toxicity (Am Rev Hesp Dis 1989;140:531). Uslng serial changes In reduced (CSH) and oxidized (GSSG) f:lutaLtllone as a marker, the abillty of RUCs to deal with a hydrogen peroxide (11.0) load was compared in vitro in preterm (n=8) and tr:ri~t (n=9) ba6igs and adults (n=10). Incubation of HBCs wlth li 0 caoscd a rapid depletion of GSH and increase of GSSG, followgd2by a recovery of GSH and fall of GSSG to initial values. A I:rcat<:r CSll depletion produced a slower GSH recovery time (r=-0.79, pcO.001). Neonatal RBCs showcd significantly less depletion and qu~cker recovery of GSli than those of adults (p<0.001). Partial inhibition of H 0 catabolism by catalase irlactivatlon produced 50% loss of int?a$ellular glutathione and slower CSH recovery (p<3.005) in a11 subjects, but recovery rernalned qu~ckcr in the babies (p<0.01). There was a positive correlation between gestational age and recovery time (r=O.G8, pc0.02). The cCfuctlvc peroxide catabolism in neonatal RBCs may partly conlpcnaatr for. ricficlencies In antioxidant defenses of the lmmature l u n g , PLATELET ACTIVATION IN 'THALASSEMIC CH1I.I)IIEN.