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Abstract

To find out different qualitative and quantitative phytochemical properties, it's in-vitro antioxidant properties and peroxidase activity of Brassica juncea var. Rugosa (record tro-50197928) with fresh and sequential solvent extracts. Methods: The leaf sample of Brassica juncea var. rugosa was collected from Manipur and the sample was dried. The dried powder was extracted using Soxhlet with the sequential solvent of non-polar to polar. Preliminary phytochemical analysis for proteins, carbohydrates, phenols, alkaloids, flavonoids, tannins, saponins, steroids, terpenoids, and quantitative estimation for total proteins, carbohydrates, alkaloids, phenols, flavonoids, and tannins. In-vitro, antioxidant activity was analyzed by using DPPH assay. Peroxidase activity was also checked. Results: The plant extracts showed the presence of a considerable amount of total alkaloids, total phenols, total flavonoids, total tannins, total proteins, total carbohydrates. The plant extracts also showed high antioxidant and peroxidase activity. Conclusions: From the study, we can conclude that the plant Brassica juncea var. rugosa has many important secondary metabolites and it has a potential for strong antioxidants. INTRODUCTION: The giant-leafed mustard, also known as "Japanese mustard" or 'Head mustard'', (B. juncea var. rugosa) belonging to the family Cruciferae has broad reddish-purple variegated leaves. It is an essential vegetable crop in Manipur. It is commonly known as 'Hangam' in Manipur. It is mainly grown in winter season as kitchen garden crops in most of the houses of Manipur, India. Usually the plant grows up to 60 cm in height. The plant requires cold climatic conditions and welldrained moist soil. It can grow in semi-shade or no shade 1 .