13C-MR Spectroscopic Imaging with Hyperpolarized [1-13C]pyruvate Detects Early Response to Radiotherapy in SCC Tumors and HT-29 Tumors

Abstract

Purpose: X-ray irradiation of tumors causes diverse effects on the tumor microenvironment, including metabolism. Recent developments of hyperpolarized 13 C-MRI enabled detecting metabolic changes in tumors using a tracer [1-13 C]pyruvate, which participates in important bioenergetic processes that are altered in cancers. Here, we investigated the effects of X-ray irradiation on pyruvate metabolism in squamous cell carcinoma (SCCVII) and colon cancer (HT-29) using hyperpolarized 13 C-MRI. Experimental Design: SCCVII and HT-29 tumors were grown by injecting tumor cells into the hind legs of mice. [1-13 C]pyruvate was hyperpolarized and injected intravenously into tumor-bearing mice, and 13 C-MR signals were acquired using a 4.7 T scanner. C-MRI could be useful for the evaluation of early response to radiotherapy.

Laser‐driven radiation: Biomarkers for molecular imaging of high dose‐rate effects

“…The advantages of this approach have been outlined by a recent work that substantiated both the solid tumor response and the effects of radiotherapy on healthy tissue in order to survey potential radiation toxicity for areas surrounding the targeted tumor. The response of different types of cancer to therapy has also been monitored using hyperpolarized magnetic resonance, notably in glioma and squamous cell carcinoma . This latter type of cancer is one of the first likely targets for radiotherapy based on short‐pulse lasers with particles of low energy.…”

Radiation Toxicity in the Central Nervous System: Mechanisms and Strategies for Injury Reduction

“…The use of positron emission tomography (PET) 36 , 13C labeled metabolites such as 13C-pyruvate 37 , and cation exchange saturation transfer (CEST) imaging 38 may be able to allow investigators and clinicians to image altered glucose uptake, energy production, and glutamate neurotransmitter fluctuations, respectively, in acute and late toxicities.…”

Integration of imaging into clinical practice to assess the delivery and performance of macromolecular and nanotechnology-based oncology therapies

“…Another study used metabolism of hyperpolarized 13 C-pyruvate as a marker of response to temozolomide treatment in glioblastoma multiforme cells [155]. Additionally, preclinical studies have shown promise for the use of hyperpolarized 13 C-pyruvate MRS imaging for evaluating radiation treatment response [156] and to assess response to hypoxia-sensitizing drugs [157]. With the variety of available 13 C-labeled substrates, hyperpolarized 13 C MRS imaging may prove to be a powerful tool for visualization of numerous cancer-related metabolic processes for early assessment of treatment response.…”