14-Day Repeated Intraperitoneal Toxicity Test of Ivermectin Microemulsion Injection in Wistar Rats

Abstract

To evaluate the safety of ivermectin microemulsion injection, 100 Wistar rats were injected intraperitoneally at 0.38 g/kg, 0.19 g/kg, and 0.1 g/kg for 14 days. The 14-day repeated toxicity test of ivermectin microemulsion injection was systematically evaluated by clinical observation, organ coefficient, hematological examination, clinical chemistry examination, and histopathological examination. The results showed that no rats died during the test. At the initial stage of treatment, the rats in the high dose group had mild clinical reaction, which disappeared after 4 days. Clinical chemistry showed that the high dose of ivermectin microemulsion could cause significant changes in ALT and LDH parameters in male rats; high and medium doses could increase the liver coefficients of male and female rats. The toxic target organ may be the liver as indicated by histopathological findings. No significant toxic injury was found in the heart, liver, spleen, lung, kidney, brain, ovary, and testes of all groups of rats. No drug-related toxic effects were found at low doses, and thus the NOVEL of ivermectin microemulsion injection was 0.19 g/kg.

A Novel Study on the Hematological and Physiological Disturbance of Ivermectin-COVID 19 Treatment Abuse in Male Wistar Rats

“…Although there is insufficient evidence to prove that the decline in hematological parameters is due to ivermectin suppression of the hematopoietic system, it is still noteworthy. According to the results of hematological parameters, rats were more sensitive to the toxicity of ivermectin, and these results are in accordance with the study of Dong et al (2020). However, the percentage of Hematocrit (%) showed a highly significant increase in positive untreated group when compared to negative control group.…”

Ensayo de toxicidad oral a dosis repetidas de 28 días de ivermectina, azitromicina y acetaminofén en ratones CD1

“…No obstante, Elzoghby y colaboradores (2015) reportaron otro tipo de consecuencias renales que difieren con nuestros datos, como la descamación y la destrucción de túbulos renales, la vacuolización glomerular e infiltra- ción leucocitaria del epitelio renal. Además, se asume que estas consecuencias son dosis dependientes debido a los reportes de seguridad de las dosis recomendadas de ivermectina ( < .2 mg/kg) con ausencia de anormalidades histológicas en hígado y riñón (Dong et al, 2020).…”

Detection of Ivermectin Toxicity Using Some Biochemical and Immunological Assays in Mice

“…Ivermectin was increase the potential of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) which leads to paralysis of the parasite, In vertebrate muscle and nerve cells of the microflora [7], it binds selectively and with high affinity to glutamate-gated chloride ion channels, acting as an agonist of the GABA and disrupting GABA-mediated CNS neuro synaptic transmission [8,9]. High doses of ivermectin or mutation in p-glycoprotein may allow ivermectin to pass through the blood-brain barrier (BBB) to cause neurotoxicity in animals manifested as incoordination, mydriasis, emesis, diarrhea, salivation, depression, ataxia, tremors, coma and death [10,11,12].…”