16S rRNA gene sequencing analysis of gut microbiome in a mini‐pig diabetes model

Abstract

Background: Currently, increasing attention is being paid to the important role of intestinal microbiome in diabetes. However, few studies have evaluated the characteristics of gut microbiome in diabetic miniature pigs, despite it being a good model animal for assessing diabetes. Methods:In this study, a mini-pig diabetes model (DM) was established by 9-month high-fat diet (HFD) combined with low-dose streptozotocin, while the animals fed standard chow diet constituted the control group. 16S ribosomal RNA (rRNA) gene sequencing was performed to assess the characteristics of the intestinal microbiome in diabetic mini-pigs. Results:The results showed that microbial structure in diabetic mini-pigs was altered, reflected by increases in levels of Coprococcus_3 and Clostridium_sensu_stricto_1, which were positively correlated with diabetes, and decreases in levels of the bacteria Rikenellaceae, Clostridiales_vadinBB60_group, and Bacteroidales_RF16_group, which were inversely correlated with blood glucose and insulin resistance. Moreover, PICRUSt-predicted pathways related to the glycolysis and Entner-Doudoroff superpathway, enterobactin biosynthesis, and the l-tryptophan biosynthesis were significantly elevated in the DM group. Conclusion:These results reveal the composition and predictive functions of the intestinal microbiome in the mini-pig diabetes model, further verifying the relationship between HFD, gut microbiome, and diabetes, and providing novel insights into the application of the mini-pig diabetes model in gut microbiome research.

Amelioration of Type 2 Diabetes Using Four Strains of Lactobacillus Probiotics: Effects on Gut Microbiota Reconstitution-Mediated Regulation of Glucose Homeostasis, Inflammation, and Oxidative Stress in Mice

“…Three of the four probiotics shared pathways such as the superpathway of glycolysis and the Entner–Doudoroff pathway, glycolysis I, glycolysis II, sucrose degradation IV, and phospholipases. The superpathway of glycolysis and the Entner–Doudoroff superpathway, as predicted by PICRUSt, saw a significant increase in T2D mice compared to the normal mice . With the onset of T2D, glycolysis typically exhibits compensatory increases in diabetic mice compared to normal mice .…”

“…49 The Parasutterella genus, a staple in the intestinal core microbiome of healthy humans and mice, is linked to diabetes, playing a potential role in bile acid maintenance and cholesterol metabolism. 50 R. ilealis has been found to impair glucose tolerance and reduce fasting insulin levels and HOMA-β values in mice. 51 Its abundance was greater in T2D mice than in normal mice.…”

“…dorei is known to reduce gut permeability and alleviate endotoxemia by amplifying tight junction protein ZO-1 expression and curbing microbial LPS production, effectively suppressing proinflammatory immune responses . The Parasutterella genus, a staple in the intestinal core microbiome of healthy humans and mice, is linked to diabetes, playing a potential role in bile acid maintenance and cholesterol metabolism R.…”

“…The superpathway of glycolysis and the Entner−Doudoroff superpathway, as predicted by PICRUSt, saw a significant increase in T2D mice compared to the normal mice. 50 With the onset of T2D, glycolysis typically exhibits compensatory increases in diabetic mice compared to normal mice. 51 At the metagenomic level, an increase in sucrose degradation-related gene expression might tilt the metabolic balance in diabetics toward the catabolic side.…”

Identifying genetic susceptibility to Aspergillus fumigatus infection using collaborative cross mice and RNA‐Seq approach

“…According to the genome data, one group increased their body weight, while the other group decreased their body weight. For gene expression analysis, the GENE-E analysis platform was used to generate a clustered heat map matrix, shown in 45 This demonstrates the ability of the CC resource to provide high-resolution mapping of QTL affecting a wide variety of traits, including susceptibility to a spectrum of infectious diseases.…”