16S rRNA genes- and metagenome-based confirmation of syntrophic butyrate-oxidizing methanogenesis enriched in high butyrate loading

Meng, Xianghui; Cao, Qin; Sun, Yong; Huang, Siyuan; Liu, Xiaofeng; Liu, Dong

doi:10.1016/j.biortech.2021.126483

Cited by 71 publications

(7 citation statements)

References 37 publications

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“…The concentrations of VFAs are usually used as the essential parameters to indicate the stability of the AD process. VFAs mainly include acetic acid, propionic acid, and butyric acid . The variation of VFAs during the whole AD process at different temperatures is shown in Figure .…”

Section: Resultsmentioning

confidence: 99%

“…VFAs mainly include acetic acid, propionic acid, and butyric acid. 29 The variation of VFAs during the whole AD process at different temperatures is shown in Figure 2 . The concentrations of VFAs reached their maximum value on the 2nd day, and the concentrations of VFAs increased along with temperature on the 2nd day, indicating that the higher the temperature, the higher the rate of hydrolysis and acidification.…”

Section: Resultsmentioning

confidence: 99%

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Effect of Temperature on Anaerobic Fermentation of Poplar Ethanol Wastewater: Performance and Microbial Communities

et al. 2023

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Temperature plays an important role in anaerobic digestion (AD), and different substrates have different optimum temperatures in AD. However, the effect of temperature on the performance of AD when cellulosic ethanol wastewater was used as a substrate was rarely reported. Therefore, the digestion characteristics of cellulosic ethanol wastewater at 25, 35, 45, and 55 °C were investigated, and the microbial communities of the sludge sample were analyzed after fermentation. The results showed that the cumulative methane production was the highest at 55 °C, 906.40 ± 50.67 mL/g VS, which was 81.06, 72.42, and 13.33% higher than that at 25, 35, and 45 °C, respectively. The content of methane was 68.13, 49.26, 70.46, and 85.84% at the terminal period of fermentation at temperatures of 25, 35, 45, and 55 °C, respectively. The testing of volatile fatty acids (VFAs) indicated that the accumulation of VFAs did not occur when the fermentation was carried out at 25, 35, and 45 °C; however, the VFA content at 55 °C was much larger than that in the three groups (25, 35, and 45 °C), and the ratio of propionic acid to acetic acid was larger than 1.4 at the late stage of fermentation, so it inhibited the fermentation. The diversity of the microbial community indicated that the floral structure and metabolic pathway of fermentation were alike at 25 and 35 °C. Firmicutes and Proteobacteria were the main flora covering the 25–55 °C-based phylum or below it. The relative abundance of Methanosaeta was the highest when fermentation temperatures were 25 and 35 °C; however, its relative abundance decreased sharply and the relative abundance of Methanosarcina increased substantially when the temperature increased from 35 to 45 °C, which indicated that Methanosarcina can exist in higher temperatures. At the same time, hydrogenotrophic methanogens such as Methanoculleus and Methanothermobacter were dominant when fermentation temperatures were 45 and 55 °C, which indicated that the metabolic pathway changed from acetoclastic methanogenesis to hydrogenotrophic methanogenesis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Effect of Temperature on Anaerobic Fermentation of Poplar Ethanol Wastewater: Performance and Microbial Communities

et al. 2023

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“…However, the genus Syntrophomonas , comprising typical butyrate-oxidizing bacteria [ 20 , 91 ], showed the highest abundance in the low OLR reactor R-1. Syntrophomonas was also described as the major syntrophic butyrate-oxidizing bacterium in a butyrate-fed system [ 92 ]. Its lower abundance in the other reactors indicates that other species took over its function there.…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Analysis of Anaerobic Microbial Communities Degrading Short-Chain Fatty Acids as Sole Carbon Sources

et al. 2023

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Analyzing microbial communities using metagenomes is a powerful approach to understand compositional structures and functional connections in anaerobic digestion (AD) microbiomes. Whereas short-read sequencing approaches based on the Illumina platform result in highly fragmented metagenomes, long-read sequencing leads to more contiguous assemblies. To evaluate the performance of a hybrid approach of these two sequencing approaches we compared the metagenome-assembled genomes (MAGs) resulting from five AD microbiome samples. The samples were taken from reactors fed with short-chain fatty acids at different feeding regimes (continuous and discontinuous) and organic loading rates (OLR). Methanothrix showed a high relative abundance at all feeding regimes but was strongly reduced in abundance at higher OLR, when Methanosarcina took over. The bacterial community composition differed strongly between reactors of different feeding regimes and OLRs. However, the functional potential was similar regardless of feeding regime and OLR. The hybrid sequencing approach using Nanopore long-reads and Illumina MiSeq reads improved assembly statistics, including an increase of the N50 value (on average from 32 to 1740 kbp) and an increased length of the longest contig (on average from 94 to 1898 kbp). The hybrid approach also led to a higher share of high-quality MAGs and generated five potentially circular genomes while none were generated using MiSeq-based contigs only. Finally, 27 hybrid MAGs were reconstructed of which 18 represent potentially new species—15 of them bacterial species. During pathway analysis, selected MAGs revealed similar gene patterns of butyrate degradation and might represent new butyrate-degrading bacteria. The demonstrated advantages of adding long reads to metagenomic analyses make the hybrid approach the preferable option when dealing with complex microbiomes.

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“…Combining with the analysis of microbial community, the abundance of Syntrophomonas and acetoclastic Methanosaeta increased, promoting the consumption of HAc and HBu. Syntrophomonas belongs to the Proteobacteria phylum, which plays a crucial role in the HBu fermentation (Meng et al 2022b). The conversion of HBu to HAc and H2 was promoted.…”

Section: Effects Of Tes Concentrations On Ad Of Kwmentioning

confidence: 99%

Effects of different acidification-resisting strategies on anaerobic digestion of kitchen waste: Methanogenic properties and microbial community shift

Hua,

Yuan,

Zhao

et al. 2024

BioRes

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Anaerobic digestion (AD) has been widely used as a promising technology for the treatment of kitchen waste (KW). The effects of several acidification-resisting methods were compared, which included the supplementation of trace elements (TEs) and zero-valent iron (ZVI) / powdered activated carbon (PAC), and the application of the sludge domesticated by acetic acid (HAc) and KW as inoculum. The results showed that the supplementation of TEs and ZVI/PAC at total solid (TS) content of 6% and optimal addition doses resulted in an increase in methane yield to 346 and 366 mL/g VS, respectively. In addition, the methane yields of 327 and 241 mL/g VS were obtained by applying the sludge domesticated with KW and HAc as inoculum, while the methane yield of the control was only 89.2 mL/g VS, representing a relative increase of 288%, 311%, 267%, and 170%. The acidification could be alleviated by applying these methods, and also the methanogenic profile was improved. Furthermore, microbial community analysis revealed that the enrichment of Methanosarcina, which enhanced the substrate utilization capacity and subsequently increased methane production, was achieved through the addition of TEs and ZVI/PAC, along with the application of sludge domesticated by KW.

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16S rRNA genes- and metagenome-based confirmation of syntrophic butyrate-oxidizing methanogenesis enriched in high butyrate loading

Cited by 71 publications

References 37 publications

Effect of Temperature on Anaerobic Fermentation of Poplar Ethanol Wastewater: Performance and Microbial Communities

Effect of Temperature on Anaerobic Fermentation of Poplar Ethanol Wastewater: Performance and Microbial Communities

Metagenomic Analysis of Anaerobic Microbial Communities Degrading Short-Chain Fatty Acids as Sole Carbon Sources

Effects of different acidification-resisting strategies on anaerobic digestion of kitchen waste: Methanogenic properties and microbial community shift

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