16α-Hydroxy Steroids II

Smith, Leland L.; Foell, Theodore; Maio, Ralph De; Halwer, Murray

doi:10.1002/jps.3030480912

Cited by 26 publications

(5 citation statements)

References 12 publications

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“…Results obtained by this method are compared (Table III) with those determined by partition chromatography (5) and agree well with a maximum deviation of 0.35% for the determination of 9a-fluoroprednisolone.…”

Section: Methodssupporting

confidence: 59%

Quantitative Colorimetric Determination of Residual 9-alpha-Fluoroprednisolone and 9-alpha-Fluorohydrocortisone in Triamcinolone Samples

Sih¹,

Pan²,

Bennett³

1960

Anal. Chem.

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A colorimetric method for the simultaneous quantitative estimation of 9a-fluoroprednisolone and 9a-fluorohydrocortisone in the presence of large quantities of triamcinolone depends upon the formation of a blue chromogen from 9a-fluoroprednisolone (Amax 625 mp) and a reddish purple chromogen from 9a-fluorohydrocortisone (Am.= 548 mp) in the presence of sulfuric acid, fructose, and cysteine. Within the range of 20 to 100 y of 9a-fluorohydrocortisone and/or 9a-fluoroprednisolone, these steroids can be determined accurately in triamcinolone samples with a precision to f3.0%. The advantage of this method over the conventional one employing partition chromatography is discussed. N THE microbiological and chemical

show abstract

Section: Methodssupporting

confidence: 59%

Quantitative Colorimetric Determination of Residual 9-alpha-Fluoroprednisolone and 9-alpha-Fluorohydrocortisone in Triamcinolone Samples

Sih¹,

Pan²,

Bennett³

1960

Anal. Chem.

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“…An aliquot of each eluate was acetylated and run in the Bush 3b system (Bush, 1961). In the case of RA (1), another aliquot of each eluate was oxidized with sodium bismuthate and run in the Smith VI system (Smith, Foell, De Maio, and Halwer, 1959), and aliquots of the most polar region from RA (1), RA (3) and Db (2) were run in the Smith II system (Smith and others, 1959). For further identification of cortisol, cortisone, 20a-dihydrocortisol, 2013-dihydrocortisol, 20adihydrocortisone, and 20P-dihydrocortisone, larger aliquots of the extracts were chromatographed and the named steroids isolated as their acetates.…”

Section: Chromatographymentioning

confidence: 99%

Catabolism and interconversion of cortisol and cortisone in human synovial tissue in vitro.

Dl¹,

West²

1969

Annals of the Rheumatic Diseases

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“…IV are reduced substantially in the first reduction step by a diffusion-controlled (4) process to the mononegative ion. In protonated solvents, generally, one reduction wave is reported for the cross-conjugated dienones (6,12,13). KabasakaHan and McGlotten (9) have reported two reduction waves for prednisone at pH's greater than 6.3 in ethanol-water solutions and one reduction wave for prednisolone in the same pH range.…”

Section: Resultsmentioning

confidence: 99%

“…In a later publication, they discussed the reduction of saturated ketosteroids (7). Cross-conjugated dienones and A4-3-ketosteroids have been determined in the presence of each other by polarography (6,12,18).…”

mentioning

confidence: 99%