17α-Ethinyl estradiol affects anxiety and shoaling behavior in adult male zebra fish (Danio rerio)

Reyhanian, Nasim; Volkova, Kristina; Hallgren, Stefan; Bollner, Tomas; Olsson, Per‐Erik; Olsén, K. Håkan; Hällström, Inger Porsch

doi:10.1016/j.aquatox.2011.05.009

Cited by 81 publications

(33 citation statements)

References 65 publications

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“…While primates and rodents have traditionally been utilized to study the genetic and neural underpinning of social interactions (Fano et al, 2001;Ribeiro Do Couto et al, 2009;Sassenrath and Chapman, 1976), zebrafish can also be used for examining both normal and aberrant social behavior (Miller and Gerlai, 2007). Shoaling, an important evolutionarily conserved behavior, has long been identified in zebrafish (Mc and Bradner, 1998;Reyhanian et al, 2011;Ward et al, 2008), representing the interaction of a number of animals moving together in coordinated movements (Buske and Gerlai, 2011b;Krause et al, 2000). In zebrafish, shoaling is an innate behavior maintained at a relatively stable and high level throughout the lifespan (Miller and Gerlai, 2007), but is also modulated by social learning (Engeszer et al, 2004).…”

Section: Introductionmentioning

confidence: 98%

Automated high-throughput neurophenotyping of zebrafish social behavior

Green

Collins

Kyzar

et al. 2012

Journal of Neuroscience Methods

158

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Section: Introductionmentioning

confidence: 98%

Automated high-throughput neurophenotyping of zebrafish social behavior

Green

Collins

Kyzar

et al. 2012

Journal of Neuroscience Methods

158

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“…Recent studies have shown that ecologically important behavioral traits, such as activity and boldness, are affected by various emerging aquatic contaminants (Reyhanian et al, 2011;Brodin et al, 2013;Dzieweczynski et al, 2016) as well as other contaminants including inorganic mercury (Pereira et al, 2016), persistent organic pollutants (Vignet et al, 2015), and mining waste leachate (Lanctôt et al, 2016). Effects on boldness and activity have also been noted in response to ongoing large-scale changes in carbon dioxide (CO 2 ) concentrations in oceans and freshwaters (Munday et al, 2009(Munday et al, , 2010Welch et al, 2014;Regan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Drug-Induced Behavioral Changes: Using Laboratory Observations to Predict Field Observations

Klaminder

Hellström

Fahlman

et al. 2016

Front. Environ. Sci.

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Behavioral assays constitute important research tools when assessing how fish respond to environmental change. However, it is unclear how behavioral modifications recorded in laboratory assays are expressed in natural ecosystems, a limitation that makes it difficult to evaluate the predictive power of laboratory-based measurements. In this study, we hypothesized that exposure to a benzodiazepine (i.e., oxazepam) increases boldness and activity in laboratory assays as well as in field assays-that is, laboratory results can be used to predict field results. Moreover, we expected the modified behavior to affect other important ecological measures such as habitat selection and home range. To test our hypothesis, we exposed European perch (Perca fluviatilis) to oxazepam and measured subsequent changes in behavioral trials both in laboratory assays and in a lake ecosystem populated with a predatory fish species, pike (Esox lucius). In the lake, the positions of both perch and pike were tracked every 3 min for a month using acoustic telemetry. In the laboratory assay, the oxazepam-exposed perch were bolder and more active than the non-exposed perch. In the lake assay, the oxazepam-exposed perch were also more bold and active, had a larger home range, and used pelagic habitats more than the non-exposed perch. We conclude that ecotoxicological behavioral assays are useful for predicting the effects of exposure in natural systems. However, although individual responses to exposure were similar in both the laboratory and field trials, effects were more obvious in the field study, mainly due to reduced variability in the behavioral measures from the lake. Hence, short-term behavioral assays may fail to detect all the effects expressed in natural environments. Nevertheless, our study clearly demonstrates that behavioral modifications observed in laboratory settings can be used to predict how fish perform in aquatic ecosystems.

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“…In other works with others toxicants, the concentrations used may be lower, but on the other hand the exposures times were also much longer, allowing the accumulation of the effects caused by the toxicants. For example in one study, adult male zebrafish were only exposed to 5 ng/L 17α-ethinyl estradiol (an active component of oral contraceptive pills) (Reyhanian et al, 2011), but the exposure time used in this study (14 days) was 32 times higher than the total time (10 h 30 min) used in this work. These comparisons highlight the sensitivity of the system used in this work, indicating that the detecting capability of behavioral changes is very high, since lower concentrations and shorter exposure times were used.…”

Section: Discussionmentioning

confidence: 98%

Resilience assessment of a biological early warning system based on the locomotor behavior of zebrafish (Danio rerio)

Fernandes

Amorim

Vasconcelos

et al. 2016

Environ Sci Pollut Res

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With the development of new tools such as biological early warning systems, it becomes extremely important to test their reliability and detection capability. This work aimed at testing the sturdiness of a video tracking system by determining whether the detection capability does not deteriorate over time, after successive exposures of the zebrafish to three different toxicants, namely sodium hypochlorite, bisphenol A, and ethanol. Zebrafish were exposed to the three tested compounds separately (one fish, one toxicant) once a day, for 1 h and 30 m over 9 days, to 9 % of the 96 h LC50 of the respective toxicant. The behavior analysis was based on nine movement descriptor parameters of the fish, namely: angular velocity; linear velocity; spatial dispersion; linear acceleration; and angular acceleration. A statistical method was developed using self-organizing map (SOM), correspondence analysis, and linear and orthogonal multiple regression models. The results indicated that the system was able to successfully detect the three toxicants. With ethanol, the detection capability was maintained, but in the case of the sodium hypochlorite and bisphenol A, a deterioration of the detection capability occurred over the 9 days. This effect may be due to the induction of detoxification mechanisms and physiological acclimation, or due to the accumulation of adverse effects caused by the repeated exposure to the toxicants. Future works, especially those focusing on the application of similar early warning systems in real-world scenarios, should regularly exchange the sentinel organisms, to avoid degradation of the detection capability, as verified with two of the three tested compounds.

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17α-Ethinyl estradiol affects anxiety and shoaling behavior in adult male zebra fish (Danio rerio)

Cited by 81 publications

References 65 publications

Automated high-throughput neurophenotyping of zebrafish social behavior

Automated high-throughput neurophenotyping of zebrafish social behavior

Drug-Induced Behavioral Changes: Using Laboratory Observations to Predict Field Observations

Resilience assessment of a biological early warning system based on the locomotor behavior of zebrafish (Danio rerio)

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