18-kDa protein as a marker to detect WSSV infection in shrimps

Sathish, S.; Selvakkumar, Chinnasamy; Hameed, A.S. Sahul; Narayanan, R. B.

doi:10.1016/j.aquaculture.2004.05.006

Cited by 12 publications

(11 citation statements)

References 23 publications

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“…However, this method does not work with haemolymph and gill homogenate. Other cost‐effective diagnostic assays using fluoresceinated microspheres and latex beads coated with anti‐WSSV serum have proved to detect the virus as early as 24 h post infection in shrimps (Sathish, Selvakkumar, Hameed & Narayanan 2004).…”

Section: Diagnostic Methods For Wssvmentioning

confidence: 99%

White Spot Syndrome Virus in cultured shrimp: A review

2007

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Shrimp is one of the main aquaculture species in the world. Different viruses affect them, which causes serious mortality to economically important species, such as Penaeus monodon, Litopenaeus vannamei and L. stylirostris, among others. White spot syndrome virus or WSSV is a highly lethal, stress‐dependent virus, which belongs to the family Nimaviridae, genus Whispovirus. Three WSSV virus isolates were first detected in 1992 in Thailand, Taiwan and China. Later, a fourth isolate of the virus was detected in the Americas in 1999. This virus has a large circular double‐stranded DNA genome with different sizes (292.9–307.2 kb), where the diverse isolates show differences in virulence. This virus infects a wide range of aquatic crustaceans by vertical and horizontal transmission, with different mortality results. The spread of infection between regions may be due to infected shrimp and carriers such as other crustaceans, seabirds, aquatic arthropods or other vectors. The aim of this work is to describe the current knowledge on the status, transmission, pathology, isolation, control and genomic characteristics of WSSV.

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Section: Diagnostic Methods For Wssvmentioning

confidence: 99%

White Spot Syndrome Virus in cultured shrimp: A review

2007

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“…Several DNA-based and immunological methods such as PCR, arbitrarily primed PCR and multilocus sequence typing, real-time PCR, and enzyme-linked immunosorbent assay (ELISA) (Song et al, 1992;Takahashi et al, 1998;Saulnier et al, 2000;Sathisha et al, 2004;Goarant et al, 2006aGoarant et al, , b, 2007 have been developed for diagnosis, detection, and characterization of shrimp pathogens. However, these diagnostic tools are often time-consuming and sometimes false positives are seen, especially with the immunological methods.…”

Section: Introductionmentioning

confidence: 99%

Establishment of loop-mediated isothermal amplification method (LAMP) for the detection ofVibrio nigripulchritudoin shrimp

Fall

Chakraborty

Kono

et al. 2008

FEMS Microbiology Letters

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LAMP is a novel method that amplifies DNA with high specificity and rapidity under isothermal conditions. In this study, using the LAMP method, a diagnostic protocol was developed for the detection of Vibrio nigripulchritudo in shrimps. Vibrio nigripulchritudo is associated with distinct shrimp diseases (vibriosis) and is considered one of the threatening pathogens in shrimp industry. After initial cloning and sequencing of the intergenic spacer region (ITS) between 16S and 23S rRNA genes of V. nigripulchritudo, a set of four primers - two inner and two outer - were designed for use in the LAMP reaction. Reaction time and temperature were optimized for 60 min at 63 degrees C, respectively. The detection limit of V. nigripulchritudo by LAMP was 10(2) CFU mL(-1) but PCR could detect up to 10(3) CFU mL(-1). The LAMP method could detect the presence of V. nigripulchritudo from heart, lymphoid organ, and muscle of experimentally infected shrimps with V. nigripulchritudo. This study established a highly sensitive and a rapid diagnostic procedure for detection of V. nigripulchritudo in shrimps. The method developed in this study could be very useful for routine shrimp disease diagnostics.

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“…The two genes that were amplified in this study were major structural proteins, which might play a major role in the pathoge~qesis of WSSV infection (14,21). Sequence comparison revealed that there was variation in the VP28 gene sequence, whereas A-T and T-A point mutation (transversion) in the VP19 sequence.…”

Section: Discussionmentioning

confidence: 80%

“…Various kinds of rapid diagnostic tools, such as gene probes (19), PCR (18) and immunological methods (20)(21)(22) have been developed to detect the spread of WSSV. The immunological methods are not so accurate and sensitive as compared to PCR but are convenient for the field level detection of WSSV.…”

Section: Indian Joumal Of Clinical Biochemistry 2005mentioning

confidence: 99%

Production of recombinant enveloped structural proteins from the Chinese WSSV isolate

Jha

2005

Indian J Clin Biochem

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The white spot syndrome virus (WSSV) is one of the deadly pathogens of penaeid shrimps and other crustaceans. The WSSV virion consists of an enveloped rod-shaped nucleocapsid enclosing a large circular double stranded DNA genome of 305 Kb with 181 open reading frames. The two major structural genes, VP19 and VP28 were amplified from the genomic DNA of Chinese isolate of WSSV and ctoned in pUCm-T vector and sub cloned in pET-30a (+) vector. The expressions of genes in E. coil (BL21) were confirmed by SDS-PAGE analysis. The clones were sequenced, submitted to the gene bank and the Xiang Shan strain of WSSV were compared with the previous reported sequence of WSSV of various regions which revealed that VP19 and VP28 gene sequences had certain differences from the sequences of similar genes of the isolate already reported. The recombinant proteins expressed, purified and characterized.

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18-kDa protein as a marker to detect WSSV infection in shrimps

Cited by 12 publications

References 23 publications

White Spot Syndrome Virus in cultured shrimp: A review

White Spot Syndrome Virus in cultured shrimp: A review

Establishment of loop-mediated isothermal amplification method (LAMP) for the detection ofVibrio nigripulchritudoin shrimp

Production of recombinant enveloped structural proteins from the Chinese WSSV isolate

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