1α,25‐Dihydroxyvitamin D<sub>3</sub> increases IGF binding protein‐5 expression in cultured osteoblasts

Schmid, Christoph; Schläpfer, I.; Gosteli, Martina; Hauri, C; Froesch, E. R.; Zapf, J.

doi:10.1016/0014-5793(96)00777-6

Cited by 13 publications

(8 citation statements)

References 16 publications

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“…Consistent with my findings for IGF-I, 1,25(OH) 2 D 3 was shown to mediate its stimulatory effect on osteoblast formation by increasing the production of IGF-I by human osteoblast cells [31] and mouse calvarial cells [32]. In addition, 1,25(OH) 2 D 3 upregulates many members of the IGFBPs including IGFBP-2, -3 and 4 in hMSC and IGFBP-5 in mouse osteoblastic cells [11,33]. On the other hand, the stimulatory effect of 1, 25(OH) 2 D 3 on IGFBP-6 is unique for NIH3T3 and has not been reported before for any osteoblastic cell line, and therefore might need further investigation.…”

Section: Discussionsupporting

confidence: 69%

Osteoblast differentiation of NIH3T3 fibroblasts is associated with changes in the IGF-I/IGFBP expression pattern

Abdallah

2006

Cellular and Molecular Biology Letters

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Abbreviations used: ALP -alkaline phosphatase; BMP-2 -Bone morphogenetic protein-2; Cbfa1/Runx2 -Core binding factor, runt domain, alfa subunit 1; Dex -Dexamethasone; GCglucocorticoid; IGFBPs -insulin like growth factor binding proteins; IGFs -insulin like growth factors; MSC -mesenchymal stem cells; OC -osteocalcin; OPN -osteopontein; RT -reverse transcriptase. Short communication OSTEOBLAST DIFFERENTIATION OF NIH3T3 FIBROBLASTS IS ASSOCIATED WITH CHANGES IN THE IGF-I/IGFBP EXPRESSION PATTERNBASEM M. ABDALLAH* Department of Biochemistry, Faculty of Science, Helwan University, Cairo, Egypt Abstract: Insulin-like growth factors (IGFs) and IGF-binding proteins (IGFBPs) are essential regulators for osteoblast proliferation and differentiation. It has been reported that Dexamethasone (Dex), an active glucocorticoid (GC) analogue, synergizes the stimulatory effect of 1,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) on osteoblast differentiation in the mouse fibroblastic cell line NIH3T3. I investigated whether this stimulatory effect is associated with changes in the expression pattern of the IGF/IGFBP system. Quantitative realtime PCR technology was used to quantify the gene expression levels of the IGF-system during osteoblast differentiation and in response to 1,25(OH) 2 D 3 or Dex alone under serum-containing and serum-free culture conditions. Interestingly, NIH3T3 was shown to express high mRNA levels of IGF-I, IGF-II and IGFBP-5, and low levels of both IGFBP-2 and -6. During osteoblast differentiation (days 6-12), IGF-I mRNA was repressed by more than 60%, while the transcript of IGFBP-5 was markedly up-regulated, by more than 50-fold. Similarly, treatment with Dex alone resulted in a dose-and time-dependent increase in the expression of IGFBP-5 and a decrease in IGF-I mRNA. Treatment with 1,25(OH) 2 D 3 alone increased the mRNA levels of IGF-I and IGFBP-6 by around 4-and 7-fold, respectively, in a dose-and time-dependent manner. In conclusion, my data demonstrated that osteoblast differentiation of NIH3T3 is associated with changes in the expression pattern of IGFs/IGFBPs,

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Section: Discussionsupporting

confidence: 69%

Osteoblast differentiation of NIH3T3 fibroblasts is associated with changes in the IGF-I/IGFBP expression pattern

Abdallah

2006

Cellular and Molecular Biology Letters

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“…Western ligand blot analyses revealed consistently the stimulation of secretion of the IGFBPs (182). 1␣,25(OH) 2 D 3 stimulates IGFBP-5 mRNA expression in cultured neonatal calvarial cells (198) and the osteoblastic UMR-106 cell line (199). Increased mRNA expression and secretion of IGFBP-3 and 4 have also been observed in human osteoblastic cells in several other studies (200 -203).…”

Section: Growth Factors In 1␣mentioning

confidence: 76%

Modulation of Growth Factor/Cytokine Synthesis and Signaling by 1α,25-Dihydroxyvitamin D3: Implications in Cell Growth and Differentiation

2002

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Distinct from its classic functions in the regulation of calcium and phosphorus metabolism as a systemic hormone, 1alpha,25-dihydroxyvitamin D(3) [1alpha,25(OH)(2)D(3)] is involved in the local control and regulation of cellular growth and differentiation in various tissues, including epidermis (keratinocytes) and bone (osteoblasts and osteoclasts). In this review, the impact of 1alpha,25(OH)(2)D(3) on growth factor/cytokine synthesis and signaling is discussed, particularly as it pertains to bone cells and keratinocytes. 1alpha,25(OH)(2)D(3) not only regulates growth factor/cytokine synthesis but may also alter growth factor signaling. Recently discovered examples for such interactions are the interactions between the vitamin D receptor and the mothers against decapentaplegic-related proteins that function downstream of TGFbeta receptors. Inhibitory effects of 1alpha,25(OH)(2)D(3) on keratinocytes through TGFbeta activation and IL-1alpha, IL-6, and IL-8 suppression may provide a rationale for its beneficial effects in the treatment of hyperproliferative skin disorders, whereas stimulatory effects through the epidermal growth factor-related family members and platelet-derived growth factor may be operative in its beneficial effects in skin atrophy and wound healing. Modulation of cytokines and growth factors by 1alpha,25(OH)(2)D(3) during bone remodeling plays an important role in the coupling of osteoblastic bone formation with osteoclastic resorption to maintain bone mass.

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“…Several hormones, including prostaglandin E 2 and parathyroid hormone, enhanced IGFBP-5 gene expression in cultured osteoblasts [34][35][36], while transforming growth factor-b, platelet-derived growth factor, and basic fibroblast growth factor each reduced IGFBP-5 mRNA abundance in the same cells [37]. IGF-I also has been shown to induce IGFBP-5 mRNA and protein synthesis in cultured osteoblasts [19,33,34,37] and in bone in transgenic mice over-expressing IGF-I in osteoblasts [38].…”

Section: Expression Of Igfbp-5 In Bone Cellsmentioning

confidence: 95%

Insulin-like growth factor binding protein-5 in osteogenesis: Facilitator or inhibitor?

Mukherjee¹,

Rotwein²

2007

Growth Hormone & IGF Research

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The insulin-like growth factors (IGFs) play a central role in controlling somatic growth in mammals and exert anabolic effects on most tissues, including bone. IGF action is mediated by the IGF-I receptor and additionally is regulated by six high-affinity IGF binding proteins (IGFBP-1 through IGFBP-6), of which IGFBP-4 and IGFBP-5 are most abundant in bone. The focus of this brief review is on the role of IGFBP-5 in bone biology. IGFBP-5 has been implicated as a pro-osteogenic factor in several studies but conversely has been shown to act as an inhibitor of bone formation, primarily by interfering with IGF actions on osteoblasts. These potentially contradictory effects of IGFBP-5 in bone are further complicated by observations indicating that IGFBP-5 additionally may function in an IGF-independent way, and may have been accentuated by differences in both experimental design and methodology among published studies. Suggestions are made for a more systematic approach to help discern the true roles of IGFBP-5 in bone physiology.

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1α,25‐Dihydroxyvitamin D₃ increases IGF binding protein‐5 expression in cultured osteoblasts

Cited by 13 publications

References 16 publications

Osteoblast differentiation of NIH3T3 fibroblasts is associated with changes in the IGF-I/IGFBP expression pattern

Osteoblast differentiation of NIH3T3 fibroblasts is associated with changes in the IGF-I/IGFBP expression pattern

Modulation of Growth Factor/Cytokine Synthesis and Signaling by 1α,25-Dihydroxyvitamin D3: Implications in Cell Growth and Differentiation

Insulin-like growth factor binding protein-5 in osteogenesis: Facilitator or inhibitor?

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1α,25‐Dihydroxyvitamin D3 increases IGF binding protein‐5 expression in cultured osteoblasts

Cited by 13 publications

References 16 publications

Osteoblast differentiation of NIH3T3 fibroblasts is associated with changes in the IGF-I/IGFBP expression pattern

Osteoblast differentiation of NIH3T3 fibroblasts is associated with changes in the IGF-I/IGFBP expression pattern

Modulation of Growth Factor/Cytokine Synthesis and Signaling by 1α,25-Dihydroxyvitamin D3: Implications in Cell Growth and Differentiation

Insulin-like growth factor binding protein-5 in osteogenesis: Facilitator or inhibitor?

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1α,25‐Dihydroxyvitamin D₃ increases IGF binding protein‐5 expression in cultured osteoblasts