2′,4′-BNA/LNA aptamers: CE-SELEX using a DNA-based library of full-length 2′-O,4′-C-methylene-bridged/linked bicyclic ribonucleotides

In vitroevolution of chemically-modified nucleic acid aptamers: Pros and cons, and comprehensive selection strategies

“…DNA aptamers containing LNA (BNA/LNA) nucleotides were developed against human thrombin using capillary electrophoresis SELEX. BNA/LNA-modified nucleotides were introduced in the primer-binding region of the library, and after selection the selected candidates were enriched by PCR using the LNA-modified primer sequence to regenerate the selected aptamer candidates using KOD polymerase 23,69 . Many aptamers showed K d values in the low nanomolar range 23,70 .…”

Smart functional nucleic acid chimeras: Enabling tissue specific RNA targeting therapy

“…[138][139][140][141][142][143][144] In 2013, Kuwahara and co-workers www.tandfonline.comreported an LNA (BNA) aptamer against thrombin using capillary electrophoresis-based SELEX (CE-SELEX) method. 145,146 …”

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

“…In addition, in order to enhance targets applicability, other variants SELEX used are: Whole bacteria-SELEX [43], blended SELEX [44], TECS-SELEX [45], complex target SELEX [46], toggle SELEX targetswitching [47], expressions cassette also known as SELEX-SAGE [48], the mirror-image SELEX or Spiegelmer technology [49], whole cell-SELEX [50]. Efficacy is the prerequisite for any aptamer-based design; several SELEX strategies such as non-SELEX or NECEEM-SELEX, CE-SELEX [51], microfluidic SELEX [52], HTS-SELEX or automated SELEX [53], FluMag-SELEX [54], in silico SELEX [55] are applicable. However, currently improvements in SELEX have been made.…”