Medicinal and metabolite compounds from jatropha species are important in protective against various diseases like hypertensive, antimalarial, dyscrasia, anemia, vertigo, ysphonia ,neoplasia, inflammation, ulcers etc. In present study of Chloroform and metanolic extracts of stem and stembark of various Jatropha species was carried out using standard in vitro models. The metabolic activity was evaluated in vitro by using three different species. The result of the study depends of concentration of the metanolic and Chloroform extracts of the stem bark of Jatropha species. This study will provide suitable opportunities for further investigation to verify these activities in vivo.