234.3: Establishing the Most Physiological Perfusion Milieu For Normothermic Ex Vivo Pancreas Perfusion in Porcine Models: How Far Have We Reached?

Ray, Samrat; Parmentier, Catherine; Kawamura, Masataka; Arulratnam, Bhranavi; Noguchi, Yuki; Nogueira, Emmanuel; Ganesh, Sujani; Selzner, Markus; Reichman, Trevor

doi:10.1097/01.tp.0000885748.57620.00

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was based on the hypothesis of augmenting the vascular flow of the graft (vasoconstriction due to prolonged CS) utilizing the anti-inflammatory and vasodilatory effects of higher carbon dioxide concentration and some promising preliminary results from our center. 16 Similarly, for dialysate, we used a higher concentration of salt (1.5 versus 1 g/L as previously described) based on lower edema and more physiological perfusion milieu achieved by us in a series of our previous perfusion experiments. 15 At the back-table, all arterial branches were tied off, and the spleen was removed.…”

Section: Methodsmentioning

confidence: 99%

Reanimating Pancreatic Grafts Subjected to Prolonged Cold Ischemic Injury Using Normothermic Ex Vivo Perfusion

Ray,

Parmentier,

Kawamura

et al. 2024

Transplantation Direct

Self Cite

View full text Add to dashboard Cite

Background. Pancreas transplant volumes are limited because of poor utilization of “extended criteria grafts.” Prolonged cold ischemia is a risk factor associated with poor allograft survival. We aimed to establish the feasibility of transplantation using grafts subjected to prolonged cold ischemia and determine whether these grafts could be optimized using normothermic ex vivo perfusion (NEVP) in a porcine model. Methods. The study population consisted of 35 to 40 kg male Yorkshire pigs in an allotransplantation model with a 3-d survival plan for recipients. Control grafts were subjected to cold storage (CS) in a University of Wisconsin solution for 21 to 24 h (n = 6), whereas the test group received an additional 3 h NEVP after CS of 21 h (n = 5). Results. The 3-d survival was 60% in the NEVP arm versus 0% in the control arm (P = 0.008; log rank). Graft parenchyma was 60% to 70% preserved in the NEVP arm at necropsy on gross appearance. In addition, the islet function was well preserved, and both the pancreas (including the islets) and the duodenal morphology were maintained histologically. The intravenous glucose tolerance test on the day of euthanasia was in the normoglycemic range for 80% of cases in the NEVP arm. Conclusions. Optimization of pancreas grafts exposed to extended CS with NEVP seems promising at rescuing and reanimating these grafts for transplantation, resulting in significantly improved survival in a porcine pancreas transplant model.

show abstract

Section: Methodsmentioning

confidence: 99%