2D-BN nanoparticles as a spectroscopic marker and drug delivery system with protection properties

Abstract

An application of 2D-BN nanoparticles as a spectroscopic marker, weak luminescent marker and anticancer drug (doxorubicin, DOX) delivery system with protection properties was studied for the LNCaP strains of cancer cells using FTIR and Raman spectroscopy for analysing the cancer cells, cells with BN, the cancer cells with DOX, and the cancer cells with BN nanoparticles loaded by DOX. Study of IR absorption and Raman spectra of the LNCaP strains of cancer cells incubated with 2D-BN nanoparticles for 1 hour showed that the 2D-BN nanoparticles could pass through the cell membrane and localize inside the membrane or close to the membrane in the cytoplasm of the cells. We registered the spectra of the disturbed lipids during the DOX-2D-BN passing through the membrane. After incubation for 2 hours and more, spectral changes in other structural components of the cell (nuclei, cytoplasm, mitochondria) can be registered. Confocal microscopy showed that a gold nanostructured support enhances the fluorescence of the cancer cells with 2D-BN as well as that with DOX, however the double action of 2D-BN and DOX on the cancer cells aggravates the emission property of the studied system. An MTT test showed that the toxicity of DOX on the 2D-BN nanoparticles is less than that on the reference cells, and at the same time the efficiency of the DOX action on the cancer cells does not change.

Synthesis, Functionalization, and Bioapplications of Two-Dimensional Boron Nitride Nanomaterials

“…The spectral changes of the cell membrane and cytoplasm, along with the cellular internalization of Dox-loaded 2D-BNNs, were used as a marker to monitor the cellular uptake of a nanocarrier (Gnatyuk et al, 2018). The luminescence of the 2D-BNNs-Dox was monitored using confocal microscopy to visualize the time-dependent cellular localization, as shown in Figure 6.…”

The effect of 2D tungsten disulfide nanoparticles on Lewis lung carcinoma cells in vitro

“…Assignment of the IR absorption bands to characteristic vibrations of the cells functional groups was done according to. [28][29][30][31][32][33] For all IR absorption spectra, the baseline correction and band intensity normalization by the Amide I band centered at 1651 cm À1 has been carried out.…”

2D nanostructures beyond graphene: preparation, biocompatibility and biodegradation behaviors

“…2D BN materials were used as spectral markers, weak luminescent markers, and protective anticancer drug delivery systems. 69…”

“…After loading with DOX, the tumor cells' inhibition effectiveness of the DOX did not change. 69 Poly-diallyldimethylammonium chloride (PDDA) stabilized PDDA-BN-GNC (gold nanocluster composite) multifunctional composites also showed excellent biocompatibility and the cell viability was greater than 90% after 48 h incubation with a high material concentration of 100 mg mL À1 . 66 The cytotoxicity of hexagonal boron nitride NS/graphene quantum dot (hBN-GQD) nanocomposites was evaluated using MTT assay.…”