3-Dimensional imaging of collagen using second harmonic generation

Cox, Guy; Kable, Eleanor; Jones, Allan S.; Fraser, Ian S.; Manconi, Frank; Gorrell, Mark D.

doi:10.1016/s1047-8477(02)00576-2

Cited by 403 publications

(350 citation statements)

References 19 publications

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“…It has been applied to investigate, for instance, cartilage [18,19], cornea [20], tendon and ligament [21,22] structure and mechanics, as well as structural disorder in intervertebral disc [23].…”

Section: Introductionmentioning

confidence: 99%

Lamellar and fibre bundle mechanics of the annulus fibrosus in bovine intervertebral disc

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Lamellar and fibre bundle mechanics of the annulus fibrosus in bovine intervertebral disc

et al. 2016

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“…In particular, SHG microscopy has been demonstrated extremely powerful to image collagen rich tissues [65] such as cornea [66,67], tendon [68,69], and arteries [70]. In particular, SHG microscopy has been mainly used for selectively investigating collagen fibres orientation and their structural changes in human dermis [71][72][73][74][75], keloid [76][77][78], fibrosis [79][80][81], thermally-treated samples [82][83][84][85][86], and also in tumour microenvironments [87][88][89][90][91][92][93].…”

Section: Introductionmentioning

confidence: 99%

From molecular structure to tissue architecture: collagen organization probed by SHG microscopy

Cicchi

Vogler

Kapsokalyvas

et al. 2012

Journal of Biophotonics

171

132

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Second‐harmonic generation (SHG) microscopy is a fantastic tool for imaging collagen and probing its hierarchical organization from molecular scale up to tissue architectural level. In fact, SHG combines the advantages of a non‐linear microscopy approach with a coherent modality able to probe molecular organization. In this manuscript we review the physical concepts describing SHG from collagen, highlighting how this optical process allows to probe structures ranging from molecular sizes to tissue architecture, through image pattern analysis and scoring methods. Starting from the description of the most relevant approaches employing SHG polarization anisotropy and forward – backward SHG detection, we then focus on the most relevant methods for imaging and characterizing collagen organization in tissues through image pattern analysis methods, highlighting advantages and limitations of the methods applied to tissue imaging and to potential clinical applications. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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“…The majority of the recent reports have focused on visualizing collagen fibers in natural tissues including skin, tendon, blood vessels, and cornea (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). A smaller body of publications has described SHG imaging of acto-myosin complexes in muscle (13,14) as well as microtubule-based structures in live cells (13,15).…”

Section: Introductionmentioning

confidence: 99%

Phase matching considerations in second harmonic generation from tissues: Effects on emission directionality, conversion efficiency and observed morphology

LaComb

Nadiarnykh

Townsend

et al. 2008

Optics Communications

156

180

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3-Dimensional imaging of collagen using second harmonic generation

Cited by 403 publications

References 19 publications

Lamellar and fibre bundle mechanics of the annulus fibrosus in bovine intervertebral disc

Lamellar and fibre bundle mechanics of the annulus fibrosus in bovine intervertebral disc

From molecular structure to tissue architecture: collagen organization probed by SHG microscopy

Phase matching considerations in second harmonic generation from tissues: Effects on emission directionality, conversion efficiency and observed morphology

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