[30] Microsomal lipid peroxidation

Buege, John A.; Aust, Steven D.

doi:10.1016/s0076-6879(78)52032-6

Cited by 11,201 publications

(5,503 citation statements)

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“…The thiobarbituric acid-reactive substances (TBARS) were measured in serum according to a modification of the spectrophotometic method of Buege and Aust (1978). Perchloric acid was added to serum samples to precipitate proteins.…”

Section: Methodsmentioning

confidence: 99%

Effects of oral micellized natural vitamin E (d-α-tocopherol) v. synthetic vitamin E (dl-α-tocopherol) in feed on α-tocopherol levels, stereoisomer distribution, oxidative stress and the immune response in piglets

Amazán

Cordero

López‐Bote

et al. 2014

Animal

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This study evaluated the strategy of supplementing oral micellized natural vitamin E (D-α-tocopherol) to either piglets and/or sows on α-tocopherol concentrations in piglets serum and tissues after weaning. One first experiment tested the influence of the vitamin E supplementation source (natural form in water v. the synthetic form in feed) and dose administered to piglets and/or sows on serum α-tocopherol concentration, α-tocopherol stereoisomer accumulation, antioxidant capacity and immune response of weaned piglets. A second experiment studied the effect of sow source and dose vitamin E supplementation on some of these parameters in piglets. Oral supplementation to sows with natural vitamin E as a micellized form (D-α-tocopherol) at the lowest dose produced a similar concentration of α-tocopherol in serum at days 2, 14 and 28 postpartum to those supplemented with threefold higher dose of the synthetic form in feed. At day 39 of age, neither piglet supplementation source nor dose significantly affected α-tocopherol accumulation in the serum, muscle, subcutaneous fat or liver. Those piglets from sows supplemented with the micellized alcohol form had higher RRR-α-tocopherol stereoisomers (P < 0.001) and lower (P < 0.001) RRS-RSS-and RSR-α-tocopherol, at day 39 of age than those from sows supplemented with the synthetic form. A predominant importance of sow over piglet vitamin E supplementation was observed on stereoisomer distribution in piglets. Low doses of oral natural vitamin E supplementation to sows or piglets did not increase the oxidative stress of piglets when compared with the use of the synthetic form in feed. Immunoglobulin levels in piglet serum at day 39 were not affected by natural vitamin E supplementation at low doses in drinking water of piglets or sows when compared with the synthetic form in feed. IgA tended to be higher (P = 0.145) at day 39 in piglets supplemented with natural vitamin E when compared with those supplemented with the synthetic form. Low doses of oral micellized natural vitamin E supplementation to sows is an interesting feeding strategy, when compared with the use of high doses of the synthetic form in feed, because it results in similar α-tocopherol concentrations, allows a predominant -R stereoisomer distribution in piglets and also maintains their oxidative status in vivo.Keywords: immune response, micellized natural α-tocopherol, oxidative status, sows and piglets, stereoisomers ImplicationsThe natural form of vitamin E is preferentially accumulated by sows and piglets. In the present study, we show that micellized D-α-tocopherol added to sows in water at threefold lower doses than the synthetic form (DL-α-tocopherol) in feed allows to reach similar α-tocopherol concentration and a predominant -R stereoisomer distribution in piglets. The present paper contributes to better understanding the oral natural vitamin E needs of the sow and piglets during gestation and lactation period.

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Section: Methodsmentioning

confidence: 99%

Effects of oral micellized natural vitamin E (d-α-tocopherol) v. synthetic vitamin E (dl-α-tocopherol) in feed on α-tocopherol levels, stereoisomer distribution, oxidative stress and the immune response in piglets

Amazán

Cordero

López‐Bote

et al. 2014

Animal

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“…thaliana plants, ecotype Col 0, and spl7 knockout plants 10 were grown as previously described. 33 The MS 1/2 medium (MS) 30 61 Chlorophyll content was determined by the trichlorometric method. 62 Gene expression by semi-quantitative and real-time quantitative PCR Total RNA was isolated from A. thaliana seedlings with Trizol Reagent (Ambion).…”

Section: Plant Growth Conditions and Treatmentsmentioning

confidence: 99%

Comparison of global responses to mild deficiency and excess copper levels in Arabidopsis seedlings

et al. 2013

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“…The formation of conjugated diene was determined by absorbance at 233 nm [6]. MDA was determined using the thiobarbituric acid reaction [12].…”

Section: Oxidative Status Determinationsmentioning

confidence: 99%

Effect of atorvastatin on paraoxonase1 (PON1) and oxidative status

Nagila

Permpongpaiboon

Tantrarongroj

et al. 2009

Pharmacological Reports

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Abstract:It has been proposed that paraoxonase1 (PON1), a high density lipoprotein (HDL)-associated esterase/lactonase, has antiatherosclerotic properties. The activity of PON1 is influenced by PON1 polymorphisms. However, the influence of PON1 polymorphisms on PON1 activity and oxidative stress in response to lipid-lowering drugs remains poorly understood. The objective of the present study was to investigate the effects of atorvastatin on PON1 activity and oxidative status. The influence of PON1 polymorphisms on PON1 activity and oxidative status in response to atorvastatin treatment was also evaluated. In total, 22 hypercholesterolemic patients were treated with atorvastatin at a dose of 10 mg/day for 3 months. Lipid profile, lipid oxidation markers (malondialdehyde (MDA), conjugated diene (CD), total peroxides (TP)), total antioxidant substance (TAS), oxidative stress index (OSI), and paraoxonase1 activity were determined before and after treatment. L55M, Q192R, and T(-107)C PON1 polymorphisms were also determined. Atorvastatin treatment significantly reduced the levels of total cholesterol (24.5%), low density lipoprotein (LDL) cholesterol (25.4%), triglycerides (24.4%), CD (4.4%), MDA (15.2%), TP (13.0%) and OSI (24.0%), and significantly increased the levels of TAS (27.3%), and PON1 activity (14.0%). Interestingly, the increase in PON1 activity and the reduction in oxidative stress in response to atorvastatin were influenced only by the PON1 T-107C polymorphism. Atorvastatin treatment improved the lipid profile, lipid oxidation, and oxidative/antioxidative status markers including the activity of PON1 towards paraoxon. These beneficial effects may be attributed to the antioxidant properties of statins and the increase in PON1 activity. The increase in PON1 activity was enhanced by the PON1 T-107C polymorphism.

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[30] Microsomal lipid peroxidation

Cited by 11,201 publications

References 37 publications

Effects of oral micellized natural vitamin E (d-α-tocopherol) v. synthetic vitamin E (dl-α-tocopherol) in feed on α-tocopherol levels, stereoisomer distribution, oxidative stress and the immune response in piglets

Effects of oral micellized natural vitamin E (d-α-tocopherol) v. synthetic vitamin E (dl-α-tocopherol) in feed on α-tocopherol levels, stereoisomer distribution, oxidative stress and the immune response in piglets

Comparison of global responses to mild deficiency and excess copper levels in Arabidopsis seedlings

Effect of atorvastatin on paraoxonase1 (PON1) and oxidative status

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