1971
DOI: 10.1016/s0076-6879(71)21033-8
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[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids

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Cited by 1,279 publications
(583 citation statements)
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References 18 publications
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“…Only a single fluorescent band was present at a density corresponding to the linear covalently continuous DNA bands of plasmid pBR322 and phage PM2 DNA centrifuged in parallel gradients in the same experiment. The invisible DNA present in the region below the main band was collected, purified and examined by electron microscopy, using a formamide version [26] of the Kleinschmidt protein-monolayer technique [31]. In addition to linear DNA, this fraction contained three types of circular molecule (see Fig.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Only a single fluorescent band was present at a density corresponding to the linear covalently continuous DNA bands of plasmid pBR322 and phage PM2 DNA centrifuged in parallel gradients in the same experiment. The invisible DNA present in the region below the main band was collected, purified and examined by electron microscopy, using a formamide version [26] of the Kleinschmidt protein-monolayer technique [31]. In addition to linear DNA, this fraction contained three types of circular molecule (see Fig.…”
Section: Resultsmentioning
confidence: 99%
“…The standard spreading procedure as described in Ref. 26 was used in a slightly modified form. The final concentrations of reagents in the spreading solution were 40% formamide, 100 mM Tris-HCl (pH 8.5), 10 mM Na2EDTA, 100 mM NaCI and 0.01% cytochrome c. DNA was added in a concentration of 0.1 /~g/ml.…”
Section: Methodsmentioning
confidence: 99%
“…Beeause they therefore belong to the same ineompatibility group, incL, pMU407-l and R471a should be related, yet at first glance the fingerprints of R47la and the pMU plasmids were notable more for their diflerenees than their similarity. Endonuclease fingerprinting may not be as useful as electron microscopic heteroduplex analysis (Davis, Simon and Davidson, 1971) or DNA-DNA hybridisation (e.g. Barth and Grinter, 1975) for determining relatedness between potentially related plasmids whieh differ both in molecular weight and in the resistance determinants they carry.…”
Section: Discussionmentioning
confidence: 99%
“…DNA from the B19 isolates JB and Br II (Table 2) was examined. It was spread on grids following the method of Davis et al ( 1971), and examined with an Elmiskop 1A (Siemens) working at 80 kV. M 13 DNA was used as a single-stranded standard, pAT153 DNA as a double-stranded standard.…”
Section: Methodsmentioning
confidence: 99%