32P-labeling test for DNA damage.

Randerath, Kurt; Reddy, M V; Gupta, Ramesh C.

doi:10.1073/pnas.78.10.6126

Cited by 481 publications

(206 citation statements)

References 22 publications

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“…The nuclease P1 enrichment version 11 and the 1-butanol extraction-mediated enrichment procedure 12 of the 32 P-postlabeling assay 13 were performed by digesting DNA samples (12.5 μg) with micrococcal nuclease (750 mU) and spleen phosphodiesterase (12.5 mU) in digestion buff er (20 mM sodium succinate, 8 mM CaCl 2 , pH 6.0) for 3 h at 37 o C in a total volume of 12.5 μl. Here, 2.5 μl of the digests were removed and diluted 1:1500 to determine the amount of normal nucleotides.…”

Section: Chemicalsmentioning

confidence: 99%

Macleaya Cordata Extract and Sangrovit Genotoxicity. Assessment in Vivo

Stiborová¹,

Vostalová²,

Zdařilová³

et al. 2008

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Background: Sanguinarine (SG) has been reported to form DNA adducts in vitro and increase the levels of DNA single strand breaks in the blood and bone marrow of mice treated intraperitoneally with SG. Recently, we showed no genotoxic eff ects of orally administrated 120 mg/kg feed Macleaya cordata extract (a mixture of sanguinarine and chelerythrine) in pigs or rats in 90-day studies. The goal of this paper was to assess the possible genotoxicity of M. cordata extract when included as a dietary admixture to rodents at concentrations providing 600 mg/kg feed and 100, 7000 or 14000 mg/kg feed Sangrovit ® (natural feed additive containing M. cordata extract and powdered M. cordata) in a 90-day pilot study. Methods and Results:The rats consumed ad libitum either the standard diet or the diets containing 367 ppm of sanguinarine and chelerythrine in M. cordata extract, and 5, 330, or 660 ppm of total alkaloids in Sangrovit ® for 90 days. The DNA adducts formation in liver was analyzed by 32 P-postlabeling technique and DNA single strand breaks in lymphocytes were evaluated by Comet assay. The results showed that M. cordata extract and/or Sangrovit ® induced no DNA damage to rat lymphocytes or hepatocytes after 90-days oral administration.Conclusions: Data from the studies described in this paper and the fact that Sangrovit ® given to the rats in our experiments were higher than the recommended dose (50 to 100 mg/kg feed), argue strongly in favour of the use of Sangrovit ® in live stock.

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Section: Chemicalsmentioning

confidence: 99%

Macleaya Cordata Extract and Sangrovit Genotoxicity. Assessment in Vivo

Stiborová¹,

Vostalová²,

Zdařilová³

et al. 2008

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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“…Catalase, but not SOD, provides signifRanderath and associates (97)(98)(99)(100)(101)(102)(103)(104)(105)(106) and it protection against ACT-and aged others (107,108) have used the phosphoechol-induced DNA nicks (82 Figure 6. The electron spin resonance (ESR) spectra of the material obtained when the DNA from rat alveolar macrophages (RAM) that have been incubated either with (A) ACT or (B) aged catechol solutions and then isolated on polycarbonate filters (92).…”

Section: Tar Radicalsmentioning

confidence: 99%

Cigarette Smoke Radicals and the Role of Free Radicals in Chemical Carcinogenicity

Pryor¹

1997

Environmental Health Perspectives

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This article consists of two parts: a brief overview of the ways in which free radicals can be involved in chemical carcinogenesis, and a review of cigarette smoke chemistry. Carcinogenesis is generally agreed to involve at least three stages: initiation, promotion, and progression. It is suggested that radicals sometimes are involved in the initiation step, either in the oxidative activation of a procarcinogen (such as benzo[alpyrene) to its carcinogenic form or in the binding of the carcinogenic species to DNA, or both. The fraction of initiation events that involve radicals, as opposed to two-electron steps, is not known, but radicals probably are involved in a substantial number, although probably not a majority, of cancer initation reactions. Promotion always involves radicals, at least to some extent. Progression probably does not normally involve radicals. The second part of this article reviews the molecular mechanisms involved in cigarette-induced tumors, particularly by aqueous cigarette tar (ACT) extracts and by a model of these solutions, aged solutions of catechol. ACT solutions as well as aged solutions of catechol contain a quinone-hydroquinone-semiquinone system that can reduce oxygen to produce superoxide and hence hydrogen peroxide and the hydroxyl radical. Both the cigarette tar radical and the catecholderived radical can penetrate viable cells, bind to DNA, and cause nicks.Environ Health Perspect 1 05(Suppl 4): 875-882 (1997)

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“…In vitro 1,2-Epoxypropane binds covalently to calf thymus DNA (Djuric et al 1986;Randerath et al 1981;Solomon et al 1988). 1,2-Epoxypropane induces DNA damage and gene mutations in bacteria; it causes gene mutations in yeasts and fungi and mitotic gene conversion in Saccharomyces cerevisiae.…”

Section: Genotoxicitymentioning

confidence: 99%

1,2‐Epoxypropane [MAK Value Documentation, 2013]

2015

The MAK‐Collection for Occupational Health and Safety

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The article contains sections titled: Toxic Effects and Mode of Action Mechanism of Action Toxicokinetics and Metabolism Absorption, distribution, elimination Metabolism Effects in Humans Single exposures Repeated exposure Local effects on skin and mucous membranes Allergenic effects Reproductive and developmental toxicity Genotoxicity Carcinogenicity Animal experiments and in vitro studies Acute toxicity Subacute, subchronic and chronic toxicity Local effects on skin and mucous membranes Allergenic effects Reproductive and developmental toxicity Genotoxicity Carcinogenicity Manifesto (MAK value/classification)

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32P-labeling test for DNA damage.

Cited by 481 publications

References 22 publications

Macleaya Cordata Extract and Sangrovit Genotoxicity. Assessment in Vivo

Macleaya Cordata Extract and Sangrovit Genotoxicity. Assessment in Vivo

Cigarette Smoke Radicals and the Role of Free Radicals in Chemical Carcinogenicity

1,2‐Epoxypropane [MAK Value Documentation, 2013]

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