3D bioprinting of a trachea-mimetic cellular construct of a clinically relevant size

Park, Jeong Hun; Ahn, Minjun; Park, Sun Hwa; Kim, Hyeonji; Bae, Mihyeon; Park, Wonbin; Hollister, Scott J.; Kim, Sung Won; Cho, Dong‐Woo

doi:10.1016/j.biomaterials.2021.121246

Cited by 32 publications

(20 citation statements)

References 55 publications

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“…A newer method, extrusion-based 3D bioprinting, has been used to create artificial structures from different materials and in different designs. This technique has shown great promise in producing heterogeneous constructs using a variety of cell types and biocompatible polymers to create cellular tracheal structures that mimic the biological and physiological function of the natural trachea [ 89 , 90 ]. Although 3D-printed products excellently mimic physiological properties, biomedical devices made with this technology are static and not intended for use under dynamic conditions.…”

Section: Resultsmentioning

confidence: 99%

Investigation of the In Vitro and In Vivo Biocompatibility of a Three-Dimensional Printed Thermoplastic Polyurethane/Polylactic Acid Blend for the Development of Tracheal Scaffolds

et al. 2023

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Tissue-engineered polymeric implants are preferable because they do not cause a significant inflammatory reaction in the surrounding tissue. Three-dimensional (3D) technology can be used to fabricate a customised scaffold, which is critical for implantation. This study aimed to investigate the biocompatibility of a mixture of thermoplastic polyurethane (TPU) and polylactic acid (PLA) and the effects of their extract in cell cultures and in animal models as potential tracheal replacement materials. The morphology of the 3D-printed scaffolds was investigated using scanning electron microscopy (SEM), while the degradability, pH, and effects of the 3D-printed TPU/PLA scaffolds and their extracts were investigated in cell culture studies. In addition, subcutaneous implantation of 3D-printed scaffold was performed to evaluate the biocompatibility of the scaffold in a rat model at different time points. A histopathological examination was performed to investigate the local inflammatory response and angiogenesis. The in vitro results showed that the composite and its extract were not toxic. Similarly, the pH of the extracts did not inhibit cell proliferation and migration. The analysis of biocompatibility of the scaffolds from the in vivo results suggests that porous TPU/PLA scaffolds may facilitate cell adhesion, migration, and proliferation and promote angiogenesis in host cells. The current results suggest that with 3D printing technology, TPU and PLA could be used as materials to construct scaffolds with suitable properties and provide a solution to the challenges of tracheal transplantation.

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Section: Resultsmentioning

confidence: 99%

Investigation of the In Vitro and In Vivo Biocompatibility of a Three-Dimensional Printed Thermoplastic Polyurethane/Polylactic Acid Blend for the Development of Tracheal Scaffolds

et al. 2023

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“…3D bioprinting has an increasingly important role in replacing damaged or diseased structural tissues with successful clinical applications in fields such as orthopedics and cosmetic repair. With respect to respiratory tissues, 3D-bioprinted tracheas and large airways have a growing potential clinical role ( Ke et al, 2019 ; Mahfouzia et al, 2021 ; Park et al, 2021 ; Huo et al, 2022 ). However, there are a number of challenges including choice of matrix material to be used in the bioprinting process as well as effective vascularization and epithelialization of the bioprinted structure ( Galliger et al, 2019 ; De Santis et al, 2021 ; Falcones et al, 2021 ).…”

Section: Overview Of Lung Regenerative Medicinementioning

confidence: 99%

What is the need and why is it time for innovative models for understanding lung repair and regeneration?

Weiss

2023

Front. Pharmacol.

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Advances in tissue engineering continue at a rapid pace and have provided novel methodologies and insights into normal cell and tissue homeostasis, disease pathogenesis, and new potential therapeutic strategies. The evolution of new techniques has particularly invigorated the field and span a range from novel organ and organoid technologies to increasingly sophisticated imaging modalities. This is particularly relevant for the field of lung biology and diseases as many lung diseases, including chronic obstructive pulmonary disease (COPD) and idiopathic fibrosis (IPF), among others, remain incurable with significant morbidity and mortality. Advances in lung regenerative medicine and engineering also offer new potential avenues for critical illnesses such as the acute respiratory distress syndrome (ARDS) which also continue to have significant morbidity and mortality. In this review, an overview of lung regenerative medicine with focus on current status of both structural and functional repair will be presented. This will serve as a platform for surveying innovative models and techniques for study, highlighting the need and timeliness for these approaches.

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“…Shortly, hydrogels with Young’s modulus between ∼10 and 30 kPa were obtained just by altering the CNF and Ca 2+ content. The experimental results showed that factors such as the direction and spacing of the bioink printed lines would affect the mechanical properties of the final scaffold ( Park et al, 2021 ). Therefore, in addition to the mechanical properties of the material itself, proper printing settings and structural design also affect the mechanical properties of the scaffold ( Monfared et al, 2021 ; Shin et al, 2021 ).…”

Section: Application In Bone and Cartilage Repairmentioning

confidence: 99%

3D printing of bone and cartilage with polymer materials

Fan

Liu²,

Wang³

et al. 2022

Front. Pharmacol.

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Damage and degeneration to bone and articular cartilage are the leading causes of musculoskeletal disability. Commonly used clinical and surgical methods include autologous/allogeneic bone and cartilage transplantation, vascularized bone transplantation, autologous chondrocyte implantation, mosaicplasty, and joint replacement. 3D bio printing technology to construct implants by layer-by-layer printing of biological materials, living cells, and other biologically active substances in vitro, which is expected to replace the repair mentioned above methods. Researchers use cells and biomedical materials as discrete materials. 3D bio printing has largely solved the problem of insufficient organ donors with the ability to prepare different organs and tissue structures. This paper mainly discusses the application of polymer materials, bio printing cell selection, and its application in bone and cartilage repair.

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3D bioprinting of a trachea-mimetic cellular construct of a clinically relevant size

Cited by 32 publications

References 55 publications

Investigation of the In Vitro and In Vivo Biocompatibility of a Three-Dimensional Printed Thermoplastic Polyurethane/Polylactic Acid Blend for the Development of Tracheal Scaffolds

Investigation of the In Vitro and In Vivo Biocompatibility of a Three-Dimensional Printed Thermoplastic Polyurethane/Polylactic Acid Blend for the Development of Tracheal Scaffolds

What is the need and why is it time for innovative models for understanding lung repair and regeneration?

3D printing of bone and cartilage with polymer materials

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