3D cell coculture tumor model: A promising approach for future cancer drug discovery

Xin, Xin; Yang, Hopen; Zhang, Fengli; Yang, Shang‐Tian

doi:10.1016/j.procbio.2018.12.028

Cited by 49 publications

(35 citation statements)

References 146 publications

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“…Cells in non-contact co-cultures are not allowed to contact and adhere together, because they are kept in distinct layers or chambers. In those methods interactions between cancer and stromal cells could be assigned to chemical mediators [ 38 ].…”

Section: Spheroids As a Type Of 3d Cell Culturesmentioning

confidence: 99%

Spheroids as a Type of Three-Dimensional Cell Cultures—Examples of Methods of Preparation and the Most Important Application

Białkowska

Komorowski

Bryszewska

et al. 2020

IJMS

237

133

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Cell cultures are very important for testing materials and drugs, and in the examination of cell biology and special cell mechanisms. The most popular models of cell culture are two-dimensional (2D) as monolayers, but this does not mimic the natural cell environment. Cells are mostly deprived of cell–cell and cell–extracellular matrix interactions. A much better in vitro model is three-dimensional (3D) culture. Because many cell lines have the ability to self-assemble, one 3D culturing method is to produce spheroids. There are several systems for culturing cells in spheroids, e.g., hanging drop, scaffolds and hydrogels, and these cultures have their applications in drug and nanoparticles testing, and disease modeling. In this paper we would like to present methods of preparation of spheroids in general and emphasize the most important applications.

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Section: Spheroids As a Type Of 3d Cell Culturesmentioning

confidence: 99%

Spheroids as a Type of Three-Dimensional Cell Cultures—Examples of Methods of Preparation and the Most Important Application

Białkowska

Komorowski

Bryszewska

et al. 2020

IJMS

237

133

View full text Add to dashboard Cite

show abstract

“…Recently, the development of glioma spheres to three-dimensional culture has prompted researchers to couple co-culture with this new technology. More in depth, artificial 3D culture platforms provide an additional dimension for cellular proliferation and interaction compared to 2D models, facilitating the spatial organization of cell morphology and cell-cell or cell-ECM signal transduction [91].…”

Section: Co-cultures Of Cerebral Organoidsmentioning

confidence: 99%

The Organoid Era Permits the Development of New Applications to Study Glioblastoma

Andreatta

Beccaceci

Fortuna

et al. 2020

Cancers

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Glioblastoma (GB) is the most frequent and aggressive type of glioma. The lack of reliable GB models, together with its considerable clinical heterogeneity, has impaired a comprehensive investigation of the mechanisms that lead to tumorigenesis, cancer progression, and response to treatments. Recently, 3D cultures have opened the possibility to overcome these challenges and cerebral organoids are emerging as a leading-edge tool in GB research. The opportunity to easily engineer brain organoids via gene editing and to perform co-cultures with patient-derived tumor spheroids has enabled the analysis of cancer development in a context that better mimics brain tissue architecture. Moreover, the establishment of biobanks from GB patient-derived organoids represents a crucial starting point to improve precision medicine therapies. This review exemplifies relevant aspects of 3D models of glioblastoma, with a specific focus on organoids and their involvement in basic and translational research.

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“…Co-culture based models are advanced cell culture techniques where tumor cells are grown together with cells such as fibroblasts or stromal cells that support their growth and development [ 85 ]. Co-cultures may be of two types: direct and indirect [ 86 ].…”

Section: Advanced 3d Cell Culture Models As Preclinical Drug Screementioning

confidence: 99%

Exploring the Potential of Drug Response Assays for Precision Medicine in Ovarian Cancer

Singh

Neal

Moatamed

et al. 2020

IJMS

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One of the major challenges in the treatment of cancer are differential responses of patients to existing standard of care anti-cancer drugs. These differential responses may, in part, be due to a diverse range of genomic, epigenomic, proteomic, and metabolic alterations among individuals suffering from the same type of cancer. Precision medicine is an emerging approach in cancer therapeutics that takes into account specific molecular alterations, environmental factors as well as lifestyle of individual patients. This approach allows clinicians and researchers to select or predict treatments that would most likely benefit the patient based on their individual tumor characteristics. One class of precision medicine tools are predictive, in vitro drug-response assays designed to test the sensitivity of patient tumor cells to existing or novel therapies. These assays have the potential to rapidly identify the most effective treatments for cancer patients and thus hold great promise in the field of precision medicine. In this review, we have highlighted several drug-response assays developed in ovarian cancer and discussed the current challenges and future prospects of these assays in the clinical management of this disease.

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3D cell coculture tumor model: A promising approach for future cancer drug discovery

Cited by 49 publications

References 146 publications

Spheroids as a Type of Three-Dimensional Cell Cultures—Examples of Methods of Preparation and the Most Important Application

Spheroids as a Type of Three-Dimensional Cell Cultures—Examples of Methods of Preparation and the Most Important Application

The Organoid Era Permits the Development of New Applications to Study Glioblastoma

Exploring the Potential of Drug Response Assays for Precision Medicine in Ovarian Cancer

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