3D Cell Spheroids as a Tool for Evaluating the Effectiveness of Carbon Nanotubes as a Drug Delivery and Photothermal Therapy Agents

Abstract

Cell spheroids (CSs) are three-dimensional models in vitro that have a microenvironment similar to tissues. Such three-dimensional cellular structures are of great interest in the field of nano biomedical research, as they can simulate information about the characteristics of nanoparticles (NPs) by avoiding the use of laboratory animals. Due to the development of areas such as bioethics and tissue engineering, it is expected that the use of such 3D cell structures will become an even more valuable tool in the hands of researchers. We present an overview of carbon nanotubes (CNTs) research on CSs in order to determine the mechanism of their incorporation into CSs, drug delivery, and photothermal therapy. We will look at such areas as the application of CNTs for medical purposes, the advantages of spheroids over classical 2D cell culture, the ways in which CNTs pass into the intercellular space, and the ways in which they are absorbed by cells in a three-dimensional environment, the use of the spheroid model for such studies as drug delivery and photothermal therapy. Thus, CSs are suitable models for obtaining additional information on the required properties of CNTs in their application in nanobiomedicine.

Plug-and-Play Lymph Node-on-Chip: Secondary Tumor Modeling by the Combination of Cell Spheroid, Collagen Sponge and T-Cells

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3D in vitro synovial hyperplasia model on polycaprolactone-micropatterned nanofibrous microwells for screening disease-modifying anti-rheumatic drugs

“…Consequently, there is active research into tissue engineering designs that can mimic a patient's microenvironment, such as 3D platforms using patient-derived cells. These platforms are capable of simulating cell-cell and cell-extracellular matrix (ECM) interactions, which are difficult to replicate in two-dimensional cell cultures, such as flasks or dishes [ [11] , [12] , [13] ].…”

Cytotoxicity of Carbon Nanotubes, Graphene, Fullerenes, and Dots

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