2017
DOI: 10.1016/j.tiv.2016.10.009
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3D engineered In vitro hepatospheroids for studying drug toxicity and metabolism

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Cited by 16 publications
(9 citation statements)
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“…Hepatocyte spheroids have been successfully applied to the prediction of hepatotoxicity and for biotransformation studies, but have not to our knowledge been extensively applied in ADME screening. [59][60][61][62] Spheroids can more fully replicate the in vivo architecture of the liver with 3D cell-cell and cell-extracellular matrix interactions, as well as a gradient of oxygen tension to encourage hepatocyte zonation. 63,64 The 3D morphology enables the cells to retain their phenotypic function over several weeks.…”
Section: Spheroidsmentioning
confidence: 99%
“…Hepatocyte spheroids have been successfully applied to the prediction of hepatotoxicity and for biotransformation studies, but have not to our knowledge been extensively applied in ADME screening. [59][60][61][62] Spheroids can more fully replicate the in vivo architecture of the liver with 3D cell-cell and cell-extracellular matrix interactions, as well as a gradient of oxygen tension to encourage hepatocyte zonation. 63,64 The 3D morphology enables the cells to retain their phenotypic function over several weeks.…”
Section: Spheroidsmentioning
confidence: 99%
“…To reproduce tissues and organs with functions that are similar to those of living organisms, in vitro evaluation using three-dimensional (3D) culture has been conducted in recent years [ 4 , 5 , 6 , 7 , 8 , 11 , 12 ]. Specifically, it has been reported that 3D culture is superior to 2D culture in terms of tissue/organ structure, cell differentiation ability, proliferation, drug toxicity, and metabolism, with results closer to those obtained in vivo [ 5 , 13 , 14 , 15 ].…”
Section: Introductionmentioning
confidence: 90%
“…It is necessary to determine the tissue/organ using 3D culture and select cells and culture techniques that are appropriate to the conditions. There are a variety of 3D culture techniques, including spheroid and organoid cultures [ 4 , 5 , 6 , 7 , 8 , 11 , 12 , 13 , 14 , 16 , 17 , 18 , 19 ], agitated culture in a bioreactor [ 20 ], hydrogel culture (ECM and synthetic polymers) [ 21 ], and cell sheet culture [ 22 , 23 , 24 ]. Whitesides et al found that hepatocyte morphology and function could be controlled using microfabrication techniques [ 25 , 26 ].…”
Section: Introductionmentioning
confidence: 99%
“…It has been shown that it is challenging to keep liver slices healthy in culture for a prolonged period [33][34][35][36][37]. To evaluate the stability of the liver slices in vitro, we tested several properties of the slices over three days of culture.…”
Section: Establishing Liver Slices For In Vitro Assaysmentioning
confidence: 99%