3D histomorphometric quantification of trabecular bones by computed microtomography using synchrotron radiation

Nogueira, Liebert Parreiras; Braz, Delson; Barroso, R.C.; Oliveira, Luis Fernando; Pinheiro, Christiano Jorge Gomes; Dreossi, Diego; Tromba, Giuliana

doi:10.1016/j.micron.2010.06.013

Cited by 24 publications

(14 citation statements)

References 35 publications

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“…These methods are non-destructive allowing 3D reconstruction based on the absorption of X-ray. Synchrotron uses a monochromatic and non-divergent beam which enables a higher spatial resolution, without artifact [14]. However, the technique is not easily Fig.…”

Section: Discussionmentioning

confidence: 99%

Analysis of β-tricalcium phosphate granules prepared with different formulations by nano-computed tomography and scanning electron microscopy

et al. 2015

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Among biomaterials used for filling bone defects, beta-tricalcium phosphate (β-TCP) is suitable in non-bearing bones, particularly in dental implantology, oral and maxillofacial surgery. When β-TCP granules are placed in a bone defect, they occupy the void 3D volume. Little is known about the 3D arrangement of the granules, which depends on the nature and size of the granules. The aim of this study was to examine the 3D architecture of porous β-TCP granules. Granules were prepared with different concentrations of β-TCP powder in slurry (10, 11, 15, 18, 21, and 25 g of β-TCP powder in distilled water). Granules were prepared by the polyurethane foam method. They were analyzed by nano-computed tomography (nanoCT) and compared with scanning electron microscopy (SEM). Commercial granules of hydroxyapatite-β-TCP prepared by the same methodology were also used. The outer and inner architectures of the granules were shown by nanoCT which evidenced macroporosity, internal porosity and microporosity between the sintered grains. Macroporosity was reduced at high concentration and conversely, numerous concave surfaces were observed. Internal porosity, related to the sublimation of the polyurethane foam, was present in all the granules. Microporosity at the grain joints was evidenced by SEM and on 2D nanoCT sections. Granules presented a heterogeneous aspect due to the different mineralization degree of the sintered powder grains in the β-TCP granules; the difference between hydroxyapatite and β-TCP was also evidenced. NanoCT is an interesting method to analyze the fine morphology of biomaterials with a resolution close to synchrotron and better than microcomputed tomography.

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Section: Discussionmentioning

confidence: 99%

Analysis of β-tricalcium phosphate granules prepared with different formulations by nano-computed tomography and scanning electron microscopy

et al. 2015

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“…Considerable research has been performed in advancing this type of imaging technique. In addition, its importance as a defect and inspection tool for specific features and new applications is growing fast in several areas such as food processing, cement compound analysis and microbiology (THOMAS et al, 1995;NOGUEIRA et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Microfocus X-ray imaging of Brazil nuts for quality control

et al. 2015

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Non-destructive quality assessment of food prior to processing is desirable in commercial facilities due to its non-invasive nature, for economic reasons and for its safety appeals. Grading Brazil nuts in this way allows for the separation of undesirable nuts to avoid contamination during the automatic nutshelling process. The aim of this study was to evaluate the feasibility of X-ray phase contrast enhanced imaging in assessing nut quality. For this goal, details of the imaging technique are described and phase contrast X-ray and microtomography imaging of nut samples are investigated. Both high quality (i.e. "sound") nuts as well as treated nuts were examined. It was concluded that both the X-ray imaging and tomography techniques have the potential to discriminate morphological features of the nut and to identify "sound" kernels from atypical ones. Larger nuts and nuts with a larger gap area between shell and kernel were concluded to have more atypical formations. Both techniques also seemed promising for use in automatic sorting lines. However, by using microtomography, the visualization of finer formations not noticeable in the X-ray images was possible. Further studies shall be carried out to investigate the nature of these formations, how they affect nut quality and their evolution with storage time. Key words: Bertholletia excelsa, fungus, microtomography, phase contrast, X-ray imaging ResumoAtualmente os grandes centros comerciais de processamento de alimentos buscam a avaliação prévia da qualidade dos produtos através de técnicas não destrutivas, devido aos apelos econômicos e de segurança alimentar. A classificação de castanhas-do-brasil através desta avaliação permite a separação das castanhas indesejáveis com a finalidade de evitar contaminações durante o processo de extração da amêndoa. O objetivo desse estudo foi avaliar a viabilidade do uso da imagem de raios-X, obtida através da técnica de contraste de fase para avaliação da qualidade da castanha. Para este objetivo, detalhes da técnica de são descritos e amostras de castanhas-do-brasil são investigadas pelas técnicas de imageamento por microtomografia e por contraste de fase de raios-X. São utilizadas castanhas saudáveis e castanhas tratadas, sendo estas últimas utilizadas com o objetivo de identificar modificações. Concluiu-se que ambas as imagens de radiografia e de tomografia de raios-X, apresentam um grande potencial para discriminar características morfológicas das castanhas e selecionar amostras saudáveis das tratadas. Dentro do conjunto de amostragem, castanhas grandes e castanhas que apresentam espaços maiores entre casca e amêndoa, mostraram formações atípicas. Ambas as técnicas mostraram-se também promissoras para serem utilizadas em linhas de produção automatizadas. Não obstante, através da microtomografia faz-se possível a visualização de características físicas mais detalhadas que não podem ser observadas usando as técnicas convencionais de imageamento de raios-X, como a radiografia convencional. A investigação da natureza d...

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“…Three-dimensional X-ray microtomography (3D µCT) has become an important tool to investigate bone morphology in osteoporosis animal model and human biopsy due its non destructive high power and resolution to indentify and quantify bone microarchitecture [2,3].…”

Section: Introductionmentioning

confidence: 99%

Segmenting 3D X-Ray microtomography using two different approaches: Morphological filters and Artificial Ant Colony

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Alvarenga

Gómez

et al. 2011

2011 Pan American Health Care Exchanges

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Three-dimensional X-Ray Micromotomography (3D µCT) has become an important tool to investigate bone morphology. Several investigators have searched a standard method for determining the optimal threshold value (optimal TH) to segment microtomographic images and quantify the bone morphology. The Conventional methods are based on subjective methods, and it is possible to obtain under or overestimated TH values. In this work, two automatic methods for segmenting 3D µCT are presented. The first one is based on Morphological Filters and Otsu's method (MFO), and the other one is based Artificial Ant Colony (AAC). Both method performances are evaluated quantitatively using a numerical phantom which mimics a trabecular bone. Besides, the proposed methods are applied to segment a microtomographic image from a rat vertebral bone. The segmented image of phantom based on AAC method showed results closer to the binary reference image of the numerical phantom and the histomorphometric parameters values presented smaller error (11.3%) than the one based on MFO (22.4%). When the methods were applied to segment a microtomographic image, the performances were compatible, and the histomorphometric parameter values present differences inferior to 9%.

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3D histomorphometric quantification of trabecular bones by computed microtomography using synchrotron radiation

Cited by 24 publications

References 35 publications

Analysis of β-tricalcium phosphate granules prepared with different formulations by nano-computed tomography and scanning electron microscopy

Analysis of β-tricalcium phosphate granules prepared with different formulations by nano-computed tomography and scanning electron microscopy

Microfocus X-ray imaging of Brazil nuts for quality control

Segmenting 3D X-Ray microtomography using two different approaches: Morphological filters and Artificial Ant Colony

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