3D microfluidic gradient generator for combination antimicrobial susceptibility testing

Sweet, Eric; Yang, Brenda; Chen, Joshua; Vickerman, Reed; Lin, Yujui; Long, Alison T.; Jacobs, Eric; Wu, Tinglin; Mercier, Camille; Jew, Ryan; Attal, Yash; Liu, Siyang; Chang, Andrew; Liu, Lin

doi:10.1038/s41378-020-00200-7

Cited by 29 publications

(25 citation statements)

References 81 publications

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“…It was found that the surface roughness of the microchannel could be reduced when the nozzle was brought closer to the print surface. Sweet et al constructed concentration gradient generators through MJM to identify optimal drug compositions [ 113 ]. The generators were a 3D microchannel network with 3 inlets and 13 outlets, as shown in Figure 8 .…”

Section: Non-mold-based Techniquesmentioning

confidence: 99%

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Fabrication of Polymer Microfluidics: An Overview

Juang

Chiu

2022

Polymers

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Microfluidic platform technology has presented a new strategy to detect and analyze analytes and biological entities thanks to its reduced dimensions, which results in lower reagent consumption, fast reaction, multiplex, simplified procedure, and high portability. In addition, various forces, such as hydrodynamic force, electrokinetic force, and acoustic force, become available to manipulate particles to be focused and aligned, sorted, trapped, patterned, etc. To fabricate microfluidic chips, silicon was the first to be used as a substrate material because its processing is highly correlated to semiconductor fabrication techniques. Nevertheless, other materials, such as glass, polymers, ceramics, and metals, were also adopted during the emergence of microfluidics. Among numerous applications of microfluidics, where repeated short-time monitoring and one-time usage at an affordable price is required, polymer microfluidics has stood out to fulfill demand by making good use of its variety in material properties and processing techniques. In this paper, the primary fabrication techniques for polymer microfluidics were reviewed and classified into two categories, e.g., mold-based and non-mold-based approaches. For the mold-based approaches, micro-embossing, micro-injection molding, and casting were discussed. As for the non-mold-based approaches, CNC micromachining, laser micromachining, and 3D printing were discussed. This review provides researchers and the general audience with an overview of the fabrication techniques of polymer microfluidic devices, which could serve as a reference when one embarks on studies in this field and deals with polymer microfluidics.

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Section: Non-mold-based Techniquesmentioning

confidence: 99%

“…( b ) Fabrication results, actual 3D μ-CGG prototype after postprocessing; hollow interior structures are visible through the semitranslucent structural material, with US quarter for scale. Adapted, with permission, from [ 113 ]. 2020, Spring Nature.…”

Section: Figurementioning

confidence: 99%

Fabrication of Polymer Microfluidics: An Overview

Juang

Chiu

2022

Polymers

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“…This limitation was addressed by creating a 3D concentration gradient generator (3D μ-CGG; Fig. 6A), which can generate symmetric 3D gradients within 5 h (Sweet et al, 2020).…”

Section: Antibiotic Researchmentioning

confidence: 99%

Microfluidic hotspots in bacteria research: A review of soil and related advances

et al. 2022

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Soil science is an inherently diverse and multidisciplinary subject that cannot develop further without the continuous introduction and promotion of emerging technologies. One such technology that is widely used in biomedicine and similar research fields, microfluidics, poses significant benefits for soil research; however, this technology is still underutilized in the field. Microfluidics offers unparalleled opportunities in soil bacterial cultivation, observation, and manipulation when compared to conventional approaches to these tasks. This review focuses on the use of microfluidics for bacteria research and, where possible, pulls from examples in the literature where the technologies were used for soil related research. The review also provides commentary on the use of microfluidics for soil bacteria research and discusses the key challenges researchers face when implementing this technology. We believe that microfluidic chips and their associated auxiliary technologies provide a prime inroad into the future of soil science research.

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“…The inner structure of microfluidic channels is functionalized with anti-EpCAM antibodies to have a clear capture of successful human cell lines of EpCAM (such as colon cancer SW480, prostate cancer PC3, and breast cancer MCF-7). Sweet et al developed a 3D µ-concentration gradient generator (µ-CGG) prototype with the help of additive manufacturing to employing a unique 3D microchannel network (Sweet et al, 2020). 3D µ-CGG is used to identify the optimal drug compositions through antimicrobial susceptibility testing for the treatment of antimicrobial-resistant (AMR) infections.…”

Section: Biomedical Application For 3d Printing Microfluidicsmentioning

confidence: 99%

3D-Printed Microfluidics and Potential Biomedical Applications

Prabhakar

Sen

Dwivedi

et al. 2021

Front. Nanotechnol.

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3D printing is a smart additive manufacturing technique that allows the engineering of biomedical devices that are usually difficult to design using conventional methodologies such as machining or molding. Nowadays, 3D-printed microfluidics has gained enormous attention due to their various advantages including fast production, cost-effectiveness, and accurate designing of a range of products even geometrically complex devices. In this review, we focused on the recent significant findings in the field of 3D-printed microfluidic devices for biomedical applications. 3D printers are used as fabrication tools for a broad variety of systems for a range of applications like diagnostic microfluidic chips to detect different analytes, for example, glucose, lactate, and glutamate and the biomarkers related to different clinically relevant diseases, for example, malaria, prostate cancer, and breast cancer. 3D printers can print various materials (inorganic and polymers) with varying density, strength, and chemical properties that provide users with a broad variety of strategic options. In this article, we have discussed potential 3D printing techniques for the fabrication of microfluidic devices that are suitable for biomedical applications. Emerging diagnostic technologies using 3D printing as a method for integrating living cells or biomaterials into 3D printing are also reviewed.

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3D microfluidic gradient generator for combination antimicrobial susceptibility testing

Cited by 29 publications

References 81 publications

Fabrication of Polymer Microfluidics: An Overview

Fabrication of Polymer Microfluidics: An Overview

Microfluidic hotspots in bacteria research: A review of soil and related advances

3D-Printed Microfluidics and Potential Biomedical Applications

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