3D models related to the publication: Morphogenesis of the inner ear at different stages of normal human development

Abstract

3D models related to the publication: Morphogenesis of the inner ear at different stages of normal human development METHODSWell-preserved human embryos between Carnegie stage (CS) 17 and the postembryonic phase during trimester 1 (approximately 6-10 weeks after fertilization) were selected from Kyoto Collection for MR microscopic imaging and phase-contrast X-ray CT (Nishimura et al, 1968;Shiota et al, 2007;O'Rahilly & Müller, 1987). The 3D PCXT image acquisition conditions are described elsewhere (Yoneyama et al., 2011). Briefly, specimens were visualized with a phase-contrast imaging system fitted with a crystal X-ray interferometer. The system was set up at the vertical wiggler beam line (PF BL14C) of the Photon Factory in Tsukuba, Japan. MR images were acquired using a 7T MR system (BioSpec 70/20 USR; Bruker Biospin MRI GmbH; Ettlingen, Germany) with a 35-mm-diameter 1H quadrature transmit-receive volume coil (T9988; Bruker Biospin MRI GmbH). PCXT and MRI data from selected embryos were analyzed precisely as serial 2D and reconstructed 3D images. The structure of the inner ear was reconstructed in all samples using Amira software version 5.4.5 (Visage Imaging; Berlin, Germany). The 3D surface models were then processed with ISE-MeshTools (Lebrun, 2014); each model was orientated, tagged and labelled using this software. All tagged surfaces are provided in .vtk format, and labels in .flg format. The 3D surface models are also provided in .ply format, and can therefore be opened with a wider range of freeware. This study was approved by The Committee of