3D perfusion bioreactor‐activated porous granules on implant fixation and early bone formation in sheep

Ding, Ming; Henriksen, Susan Snoek; Martinetti, Roberta; Overgaard, Søren

doi:10.1002/jbm.b.33783

Cited by 2 publications

(2 citation statements)

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“…Cylindrical defects were created at both medial and lateral condyle of the distal femurs, and implants were inserted. The sheep were kept for a 12-week observation period before sacrificed, and implants were harvested for analysis 20 without filling. Four implants were inserted in each sheep and interventions were compared to their own control within each sheep.…”

Section: Methodsmentioning

confidence: 99%

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Efficacy of marine bioactive compound fucoidan for bone regeneration and implant fixation in sheep

Nielsen

Mikkelsen

Ptak

et al. 2021

J Biomedical Materials Res

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The need for a substitute for allograft and autograft is rising as bone graft surgeries exceed available supplies. We investigated the efficacy of the low‐molecular weight marine bioactive compound fucoidan (FUC) on bone regeneration and implant fixation in seven female sheep, as FUC has shown great promise as a bone substitute. Titanium implants were inserted bilaterally in the distal femurs to test three hydroxyapatite/fucoidan (HA/FUC) groups and compared to allograft. The HA was coated with either 500 or 1500 μg of FUC, obtained by microwave‐assisted chemical extraction, or 500 μg of FUC obtained by an enzyme‐assisted extraction method. The concentric 2‐mm gap around the implant was filled with either one of the HA/FUCs or allograft from the donor sheep. After 12 weeks, implant–bone blocks were harvested and divided into three parts for mechanical push‐out testing, immunohistochemistry, and micro‐CT and histomorphometry. Pronounced bone formations were observed by micro‐CT and histomorphometry in all groups, but higher bone volume fractions were seen in the allograft group compared to the three HA/FUC groups. The trabecular thickness, trabecular separation, and architectural anisotropy were all significantly higher in the allograft group compared to the three HA/FUC groups. In conclusion, adequate bone formation was observed in all groups, although the bone formation was significantly greater in the allograft group. Also, no significant differences existed in the shear mechanical properties between groups, suggesting that the combination of HA and FUC can achieve a similar fixation strength to allograft in this model.

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Section: Methodsmentioning

confidence: 99%

“…Cylindrical defects were created at both medial and lateral condyle of the distal femurs, and implants were inserted. The sheep were kept for a 12‐week observation period before sacrificed, and implants were harvested for analysis 20 …”

Section: Methodsmentioning

confidence: 99%

Efficacy of marine bioactive compound fucoidan for bone regeneration and implant fixation in sheep

Nielsen

Mikkelsen

Ptak

et al. 2021

J Biomedical Materials Res

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Assessment of activated porous granules on implant fixation and early bone formation in sheep

Ding

Henriksen

Theilgaard

et al. 2016

Journal of Orthopaedic Translation

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SummaryBackground/ObjectiveDespite recent progress in regeneration medicine, the repair of large bone defects due to trauma, inflammation and tumor surgery remains a major clinical challenge. This study was designed to produce large amounts of viable bone graft materials in a novel perfusion bioreactor to promote bone formation.MethodsCylindrical defects were created bilaterally in the distal femurs of sheep, and titanium implants were inserted. The concentric gap around the implants was randomly filled either with allograft, granules, granules with bone marrow aspirate (BMA) or bioreactor activated granule (BAG). The viable BAG consisted of autologous bone marrow stromal cells (BMSCs) seeded upon porous scaffold granules incubated in a 3D perfusion bioreactor for 2 weeks prior to surgery. 6 weeks after, the bone formation and early implant fixation were assessed by means of micro-CT, histomorphometry, and mechanical test.ResultsMicroarchitectural analysis revealed that bone volume fraction and trabecular thickness in the allograft were not statistically different than those (combination of new bone and residue of granule) in the other 3 groups. The structure of the allograft group was typically plate-like, while the other 3 groups were combination of plate and rod. Histomorphometry showed that allograft induced significantly more bone and less fibrous tissue in the concentric gap than the other 3 granule groups, while the bone ingrowth to implant porous surface was not different. No significant differences among the groups were found regarding early implant mechanical fixation.ConclusionIn conclusion, despite nice bone formation and implant fixation in all groups, bioreactor activated graft material did not convincingly induce early implant fixation similar to allograft, and neither bioreactor nor by adding BMA credited additional benefit for bone formation in this model.

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3D perfusion bioreactor‐activated porous granules on implant fixation and early bone formation in sheep

Cited by 2 publications

References 61 publications

Efficacy of marine bioactive compound fucoidan for bone regeneration and implant fixation in sheep

Efficacy of marine bioactive compound fucoidan for bone regeneration and implant fixation in sheep

Assessment of activated porous granules on implant fixation and early bone formation in sheep

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