3D printable acrylate polydimethylsiloxane resins for cell culture and drug testing

Villata, Simona; Canta, Marta; Baruffaldi, Désirée; Pavan, Alice; Chiappone, Annalisa; Pirri, Candido; Frascella, Francesca; Roppolo, Ignazio

doi:10.1039/d3bm00152k

Cited by 8 publications

(8 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A comparison between the two synthesis protocols was performed on GelMA derived from Type A and B gelatin with a High DoF, as it is found to be the most widely used in literature for bioprinting applications due to its ability to maintain good printability and promotion of cell proliferation (W. Liu et al, 2017;Ning et al, 2020;Schulik et al, 2023;Villata et al, 2023). As indicated by DoF measurements (Figure 6A), GelMA derived from Type A gelatin exhibits DoFs in the range between 90% and 95%, 94.73% ± 0.31% and 91.66% ± 0.17% for Method 1 and 2 syntheses, respectively, which can be considered similar in terms of performance.…”

Section: Synthesis Protocols Comparisonmentioning

confidence: 99%

“…Gelatin Methacryloyl (GelMA), one of the most widely used bioink (Shi et al, 2023), is obtained by reacting gelatin and methacrylic anhydride (MAA). Different sources of gelatin are commonly used, however, the most employed in bioengineering applications are Type B bovine gelatin (Pahoff et al, 2019;Vassallo et al, 2022;Villata et al, 2023) and Type A porcine gelatin (González-Gamboa et al, 2022;J. Y. Lee et al, 2023 Den Bulcke et al, 2000) proposed the first synthesis method for GelMA, which involved the use of PBS as solvent and a simple dropwise addition of MAA to the heated solution.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

GelMA synthesis and sources comparison for 3D multimaterial bioprinting

Gaglio,

Baruffaldi,

Pirri

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Gelatin Methacryloyl (GelMA) is one of the most used biomaterials for a wide range of applications, such as drug delivery, disease modeling and tissue regeneration. GelMA is obtained from gelatin, which can be derived from different sources (e.g., bovine skin, and porcine skin), through substitution of reactive amine and hydroxyl groups with methacrylic anhydride (MAA). The degree of functionalization (DoF) can be tuned by varying the MAA amount used; thus, different protocols, with different reaction efficiency, have been developed, using various alkaline buffers (e.g., phosphate-buffered saline, DPBS, or carbonate-bicarbonate solution). Obviously, DoF modulation has an impact on the final GelMA properties, so a deep investigation on the features of the obtained hydrogel must be carried on. The purpose of this study is to investigate how different gelatin sources and synthesis methods affect GelMA properties, as literature lacks direct and systematic comparisons between these parameters, especially between synthesis methods. The final aim is to facilitate the choice of the source or synthesis method according to the needs of the desired application. Hence, chemical and physical properties of GelMA formulations were assessed, determining the DoFs, mechanical and viscoelastic properties by rheological analysis, water absorption by swelling capacity and enzymatic degradation rates. Biological tests with lung adenocarcinoma cells (A549) were performed. Moreover, since 3D bioprinting is a rapidly evolving technology thanks to the possibility of precise deposition of cell-laden biomaterials (bioinks) to mimic the 3D structures of several tissues, the potential of different GelMA formulations as bioinks have been tested with a multi-material approach, revealing its printability and versatility in various applications.

show abstract

Section: Synthesis Protocols Comparisonmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

GelMA synthesis and sources comparison for 3D multimaterial bioprinting

Gaglio,

Baruffaldi,

Pirri

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…[ 24,25 ] However, there is a high demand for various functional materials such as soft ‐like PDMS materials or combined hydrophilic and hydrophobic materials for spatial wettability, that require higher viscous precursors. [ 26,27 ]…”

Section: Introductionmentioning

confidence: 99%

“…[24,25] However, there is a high demand for various functional materials such as soft -like PDMS materials or combined hydrophilic and hydrophobic materials for spatial wettability, that require higher viscous precursors. [26,27] Two-photon lithography (TPL) is an emerging tool for highresolution 3D printing, where a photoinitiator, e.g., for a radical polymerization, is activated by two-photon absorption (TPA). TPL is arguably among the AM techniques with the highest possible resolution with feature sizes below 100 nm.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Embedded Microfluidic Chips with Single Micron Resolution Using Two‐Photon Lithography

Luitz,

Kırpat Konak,

Sherbaz

et al. 2023

Adv Materials Technologies

View full text Add to dashboard Cite

Two‐photon lithography (TPL) is an advanced high‐resolution additive manufacturing technique for objects with feature sizes between 100 nanometers to tens of micrometers and an overall footprint of up to hundreds of micrometers. With recent advances in the TPL technique, writing speeds are becoming faster, rendering the method feasible to print high‐resolution microfluidic chips with a footprint in the centimeter range within a reasonable time frame. In this work, a process flow to fabricate embedded microfluidic chips with channel diameters down to 30 µm is developed. To address the particular difficulty of washing the embedded channels free of uncured material, introduces a developing scheme based on a 3D printed chip‐to‐world‐interface to connect the chips to a pressure‐driven pump. This setup is leakage‐free up to a pressure of 6.9 bar for faster and safer development of embedded microfluidic devices. It manufactures meander chips with channel lengths up to 20 cm, droplet generator chips, and cell sorting chips based on deterministic lateral displacement with pillar diameters of 30 µm and pillar spacing of 4 µm. TPL of microfluidic chips will enable rapid manufacturing of novel designs, significantly reducing concept‐to‐chip times with high resolution in a reasonable amount of time.

show abstract

“…In biomedical applications, 3D printable acrylate PDMS has proven more suitable for in vitro drug testing and cell culturing of lung and skin epithelium compared with PDMS. 3 A porous titanium coating on PDMS, applied using the cold spray technique, enhances cell adhesion and proliferation, alkaline phosphatase production, and calcium deposition of MG63 cells. 4 Carbon implantation on PDMS using plasma processing facilitates covalent protein immobilization, thereby enhancing the attachment and spread of MG63 osteosarcoma cells.…”

Section: Introductionmentioning

confidence: 99%

Covalent bonding of quaternary ammonium compounds and zwitterionic polymer functional layers on polydimethylsiloxane against Escherichia Coli adhesion

Chen,

Lee,

et al. 2023

J Biomater Appl

View full text Add to dashboard Cite

Quaternary ammonium compounds (QACs) are recognized by the World Health Organization as a useful disinfectant against microbes. The synergistic effect of zwitterionic polymers with QACs as antimicrobial agents rather than QACs alone is yet to be investigated. A potential strategy is the use of covalent bonding to halt the release of minute antibacterials and a hierarchy of functional layers to detain and annihilate microbes. The strategy was tested on a polydimethylsiloxane (PDMS) surface on which quaternized poly(2-dimethylaminoethyl methacrylate) (qDMA+) and sulfobetaine (SBMA) were hierarchically functionalized. Attenuated total reflectance Fourier transform infrared analysis confirmed the quaternization of DMA to qDMA+, grafting of qDMA + on PDMS (PDMS-qDMA+), and grafting of the SBMA overlayer on PDMS-qDMA+ (PDMS-qDMA+-SB). Contact angle measurement showed that PDMS-qDMA + exhibited the lowest contact angle (26.2 ± 2.9°) compared with the hydrophobic PDMS (115.2 ± 1.6°), but that of PDMSqDMA+-SB increased to 56.3 ± 1.3°. The Escherichia coli survival count revealed that PDMS-qDMA+ and PDMS-qDMA+-SB exhibited significantly greater bactericidal ability than PDMS. Confocal laser scanning microscopy revealed fewer dead bacteria on PDMS-qDMA+-SB than on PDMS-qDMA+. Scanning electron microscopy demonstrated that E. coli was disintegrated on the functionalized surface via dual-end cell lysis. To the best of our knowledge, this is the first observation of this type of process. The results confirmed the potent antibacterial and cell disruption activities of the qDMA+ and SBMA modified PDMS surface.

show abstract

3D printable acrylate polydimethylsiloxane resins for cell culture and drug testing

Abstract: 3D printed Acrilate PDMS-based devices to design stretchable platforms for cell culture and drug testing.

Cited by 8 publications

References 57 publications

GelMA synthesis and sources comparison for 3D multimaterial bioprinting

GelMA synthesis and sources comparison for 3D multimaterial bioprinting

Fabrication of Embedded Microfluidic Chips with Single Micron Resolution Using Two‐Photon Lithography

Covalent bonding of quaternary ammonium compounds and zwitterionic polymer functional layers on polydimethylsiloxane against Escherichia Coli adhesion

Contact Info

Product

Resources

About