2020
DOI: 10.2144/btn-2019-0160
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3D-Printable Cell Crowding Device Enables Imaging of Live Cells in Compression

Abstract: We designed and fabricated, using low-cost 3D printing technologies, a device that enables direct control of cell density in epithelial monolayers. The device operates by varying the tension of a silicone substrate upon which the cells are adhered. Multiple devices can be manufactured easily and placed in any standard incubator. This allows long-term culturing of cells on pretensioned substrates until the user decreases the tension, thereby inducing compressive forces in plane and subsequent instantaneous cell… Show more

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