3D-printed bioreactors for DNA amplification: application to companion diagnostics

Pantazis, Alexandros; Papadakis, George; Parasyris, Konstantinos; Stavrinidis, A.; Gizeli, Electra

doi:10.1016/j.snb.2020.128161

Cited by 19 publications

(19 citation statements)

References 35 publications

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“…This work presents a low-cost, portable, time-saving, 3D-printed device capable of performing the simultaneous determination of two different anticancer drugs widely used in chemotherapy, opening new perspectives on potential clinical applications [23]. Regarding the employment of FDM technology for the manufacture of point-of-care (POC) devices, Pantazis et al [24] developed a 3D-printed bioreactor able to perform loopmediated isothermal amplification (LAMP) on DNA collected from saliva samples to monitor the CYP2C19×2 mutation. This mutation is involved in the metabolism of the drug clopidogrel (Plavix), adopted for cardiovascular diseases treatment.…”

Section: Materials Extrusion For Biomedical Applicationsmentioning

confidence: 99%

“…Moreover, printable biomaterials can interact with cells by physical and chemical binding at different levels, from individual cells up to a single molecule as a function of time and system dimension [74]. In this scenario, the investigation of the interactions between liv- Regarding the employment of FDM technology for the manufacture of point-of-care (POC) devices, Pantazis et al [24] developed a 3D-printed bioreactor able to perform loop-mediated isothermal amplification (LAMP) on DNA collected from saliva samples to monitor the CYP2C19×2 mutation. This mutation is involved in the metabolism of the drug clopidogrel (Plavix), adopted for cardiovascular diseases treatment.…”

Section: Materials Extrusion For Biomedical Applicationsmentioning

confidence: 99%

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3D Printing Technologies in Biosensors Production: Recent Developments

2022

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Recent advances in 3D printing technologies and materials have enabled rapid development of innovative sensors for applications in different aspects of human life. Various 3D printing technologies have been adopted to fabricate biosensors or some of their components thanks to the advantages of these methodologies over the traditional ones, such as end-user customization and rapid prototyping. In this review, the works published in the last two years on 3D-printed biosensors are considered and grouped on the basis of the 3D printing technologies applied in different fields of application, highlighting the main analytical parameters. In the first part, 3D methods are discussed, after which the principal achievements and promising aspects obtained with the 3D-printed sensors are reported. An overview of the recent developments on this current topic is provided, as established by the considered works in this multidisciplinary field. Finally, future challenges on the improvement and innovation of the 3D printing technologies utilized for biosensors production are discussed.

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Section: Materials Extrusion For Biomedical Applicationsmentioning

confidence: 99%

Section: Materials Extrusion For Biomedical Applicationsmentioning

confidence: 99%

3D Printing Technologies in Biosensors Production: Recent Developments

2022

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“…The methods can be grouped into the following categories: (i) enzyme mediated, exploiting the recycle of a single event by the biocatalytic reaction mediated; (ii) nanomaterial-based, exploiting the high surface area of nanoparticles for the high loading of DNA probes [53]; (iii) nucleic acid-based approaches, implementing the local isothermal amplification of the DNA copies before quantification [161]. Of course, to implement most of these portable nucleic acid sensing approaches, the opportunity provided by the printing approach has been exploited, particularly in terms of the integration of the sensing elements with more complex microfluidics polymers or paper [185], [190,191]. This is essential for an accurate quantification of those targets, enabling the possibility to combine sample preparation, purification, amplification and final sensing in the same portable chip [192,193].…”

Section: Nucleic Acidsmentioning

confidence: 99%

Printed Electrochemical Biosensors: Opportunities and Metrological Challenges

2020

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Printed electrochemical biosensors have recently gained increasing relevance in fields ranging from basic research to home-based point-of-care. Thus, they represent a unique opportunity to enable low-cost, fast, non-invasive and/or continuous monitoring of cells and biomolecules, exploiting their electrical properties. Printing technologies represent powerful tools to combine simpler and more customizable fabrication of biosensors with high resolution, miniaturization and integration with more complex microfluidic and electronics systems. The metrological aspects of those biosensors, such as sensitivity, repeatability and stability, represent very challenging aspects that are required for the assessment of the sensor itself. This review provides an overview of the opportunities of printed electrochemical biosensors in terms of transducing principles, metrological characteristics and the enlargement of the application field. A critical discussion on metrological challenges is then provided, deepening our understanding of the most promising trends in order to overcome them: printed nanostructures to improve the limit of detection, sensitivity and repeatability; printing strategies to improve organic biosensor integration in biological environments; emerging printing methods for non-conventional substrates; microfluidic dispensing to improve repeatability. Finally, an up-to-date analysis of the most recent examples of printed electrochemical biosensors for the main classes of target analytes (live cells, nucleic acids, proteins, metabolites and electrolytes) is reported.

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“…Loop-mediated isothermal amplification (LAMP), (11) which is one of the nucleic acid amplification methods, is very useful for on-site nucleic acid testing since it is capable of rapid nucleic acid amplification by isothermal heating. LAMP can be adapted to various detection methods [e.g., turbidimetry (12) and colorimetry (13) ] as well as to the fluorescence detection method, (14,15) and LAMP enables us to discriminate the presence or absence of gene amplification visually. (16)(17)(18) Moreover, isothermal heating enables us to simplify the system configuration.…”

Section: Introductionmentioning

confidence: 99%

Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification

Ishii¹,

Morioka²,

Mizumoto³

et al. 2022

Sensors and Materials

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We developed a portable fluorescence microplate reader equipped with an indium tin oxide (ITO) glass heater for nucleic acid testing based on loop-mediated isothermal amplification (LAMP). The components used for detection (e.g., LEDs, optical filters, the microwell plate, the ITO glass heater, and photodiodes) are arranged in parallel on a straight line. This optical placement allows us to simultaneously heat nine sample solutions and monitor the fluorescence intensity amplified by the LAMP reaction despite the analysis instrument being handheld. This simultaneous monitoring was demonstrated by the LAMP reaction using positive and negative controls. In addition, the microplate reader was applied to the variety discrimination of rice, and Koshihikari rice was successfully discriminated from four types of milled rice using the microplate reader. The developed microplate reader will be useful for on-site nucleic acid testing because it can be operated using batteries and is portable.

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3D-printed bioreactors for DNA amplification: application to companion diagnostics

Cited by 19 publications

References 35 publications

3D Printing Technologies in Biosensors Production: Recent Developments

3D Printing Technologies in Biosensors Production: Recent Developments

Printed Electrochemical Biosensors: Opportunities and Metrological Challenges

Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification

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