3D–printed biphasic calcium phosphate scaffolds coated with an oxygen generating system for enhancing engineered tissue survival

Touri, Maria; Moztarzadeh, Fathollah; Osman, Noor Azuan Abu; Dehghan, Mohammad Mehdi; Mozafari, Masoud

doi:10.1016/j.msec.2017.11.037

Cited by 85 publications

(64 citation statements)

References 46 publications

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“…The 10-day oxygen-release kinetics analysis showed no indication of a burst of oxygen (Fig. 7) 99 . Osteoblasts were then seeded onto the scaffold and cultured for up to 7 days.…”

Section: Bone Tissue Engineeringmentioning

confidence: 95%

“…Figure 7 shows the results from a study in which a biphasic calcium phosphate scaffold was fabricated with 60% hydroxyapatite (HA) and 40% beta-tricalcium phosphate (β-TCP) (Fig. 7) 99 . The scaffold was then coated with polycaprolactone (PCL) and CPO incorporated.…”

Section: Bone Tissue Engineeringmentioning

confidence: 99%

“…d Laser confocal microscopy images of osteoblast cells on (i) an uncoated BCP scaffold, (ii), (iii), and (iv) CPO-PCL BCP scaffolds coated with 1, 3, and 5% CPO, respectively. Modified and reprinted with permission from Touri M. et al 99 Copyright 2018, Elsevier oxygen-releasing biomaterials have been shown to regenerate intervertebral discs. Inadequate nutrient and oxygen supply to disc cells, or nucleus pulposus (NP) cells, is one of the main causes of disc degeneration.…”

Section: Intervertebral Disc Regenerationmentioning

confidence: 99%

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Breathing life into engineered tissues using oxygen-releasing biomaterials

et al. 2019

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Engineering three-dimensional (3D) tissues in clinically relevant sizes have demonstrated to be an effective solution to bridge the gap between organ demand and the dearth of compatible organ donors. A major challenge to the clinical translation of tissue-engineered constructs is the lack of vasculature to support an adequate supply of oxygen and nutrients post-implantation. Previous efforts to improve the vascularization of engineered tissues have not been commensurate to meeting the oxygen demands of implanted constructs during the process of homogeneous integration with the host. Maintaining cell viability and metabolic activity during this period is imperative to the survival and functionality of the engineered tissues. As a corollary, there has been a shift in the scientific impetus beyond improving vascularization. Strategies to engineer biomaterials that encapsulate cells and provide the sustained release of oxygen over time are now being explored. This review summarizes different types of oxygen-releasing biomaterials, strategies for their fabrication, and approaches to meet the oxygen requirements in various tissue engineering applications, including cardiac, skin, bone, cartilage, pancreas, and muscle regeneration.

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“…The 10-day oxygen-release kinetics analysis showed no indication of a burst of oxygen (Fig. 7) 99 . Osteoblasts were then seeded onto the scaffold and cultured for up to 7 days.…”

Section: Bone Tissue Engineeringmentioning

confidence: 95%

Section: Bone Tissue Engineeringmentioning

confidence: 99%

Section: Intervertebral Disc Regenerationmentioning

confidence: 99%

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Breathing life into engineered tissues using oxygen-releasing biomaterials

et al. 2019

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“…Touri et al prepared 3D-printed scaffolds composed of 60% HAp and 20% β-TCP using robocasting method (a direct-write assembly), and further, the produced scaffolds were coated with various ratios of an oxygen releasing agent (i.e., calcium peroxide) encapsulated within PCL matrix, which was used for in situ production of oxygen at the implanted sites. The results showed sustained release of oxygen (dependent on the concentration) that could promote bone ingrowth with enhanced osteoblast cell viability and proliferation under hypoxic conditions (Touri et al, 2018). Kim et al printed PLGA/β-TCP scaffolds with simple and complex design using FDM and coated them with HAp.…”

Section: Hap/mixed Components Printed Materialsmentioning

confidence: 99%

Additive Manufacturing Methods for Producing Hydroxyapatite and Hydroxyapatite-Based Composite Scaffolds: A Review

et al. 2019

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Hydroxyapatite (HAp) has been considered for decades an ideal biomaterial for bone repair due to its compositional and crystallographic similarity to bioapatites in hard tissues. However, fabrication of porous HAp acting as a template (scaffold) for supporting bone regeneration and growth has been a challenge to biomaterials scientists. The introduction of additive manufacturing technologies, which provide the advantages of a relatively fast, precise, controllable, and potentially scalable fabrication process, has opened new horizons in the field of bioceramic scaffolds. This review focuses on three-dimensional-printed HAp scaffolds and related composite systems, where the calcium phosphate phase is combined with other ceramics or polymers improving the mechanical properties and/or imparting special extra-functionalities. The main applications of three-dimensional-printed HAp scaffolds in bone tissue engineering are presented and discussed; furthermore, this review also emphasizes the most recent achievements toward the development and testing of multifunctional HAp-based systems combining multiple properties for advanced therapy (e.g., bone regeneration, antibacterial effect, angiogenesis, and cancer treatment).

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“…3 Moreover, tissue hypoxia (deficiency in oxygen) often occurs after the destruction of the vascular tissue and this condition can result in cell death and necrosis. 4 The tensions of oxygen below 1% inhibit glucose uptake and lactate production, as well as cellular ribonucleic acid (RNA) synthesis.…”

Section: Introductionmentioning

confidence: 99%

<p>Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications</p>

Morais¹,

Wang²,

Vieira³

et al. 2020

IJN

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Background: The facile preparation of oxygen-generating microparticles (M) consisting of Polycaprolactone (PCL), Pluronic F-127, and calcium peroxide (CPO) (PCL-F-CPO-M) fabricated through an electrospraying process is disclosed. The biological study confirmed the positive impact from the oxygen-generating microparticles on the cell growth with high viability. The presented technology could work as a prominent tool for various tissue engineering and biomedical applications. Methods: The oxygen-generated microparticles fabricated through electrospraying processes were thoroughly characterization through various methods such as X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) analysis, and scanning electron microscopy (SEM)/SEM-Energy Dispersive Spectroscopy (EDS) analysis. Results: The analyses confirmed the presence of the various components and the porous structure of the microparticles. Spherical shape with spongy characteristic microparticles were obtained with negative charge surface (ζ =-16.9) and a size of 17.00 ± 0.34 μm. Furthermore, the biological study performed on rat chondrocytes demonstrated good cell viability and the positive impact of increasing the amount of CPO in the PCL-F-CPO-M. Conclusion: This technological platform could work as an important tool for tissue engineering due to the ability of the microparticles to release oxygen in a sustained manner for up to 7 days with high cell viability.

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3D–printed biphasic calcium phosphate scaffolds coated with an oxygen generating system for enhancing engineered tissue survival

Cited by 85 publications

References 46 publications

Breathing life into engineered tissues using oxygen-releasing biomaterials

Breathing life into engineered tissues using oxygen-releasing biomaterials

Additive Manufacturing Methods for Producing Hydroxyapatite and Hydroxyapatite-Based Composite Scaffolds: A Review

<p>Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications</p>

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