3D printed imaging platform for portable cell counting

Awate, Diwakar M.; Pola, Cícero C.; Shumaker, Erica; Gomes, Carmen; Juárez, Jaime J.

doi:10.1039/d1an00778e

Cited by 3 publications

(4 citation statements)

References 35 publications

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“…In addition, for cardiovascular organoids, electrical pacing of cardiomyocytes is dependent on temperature, and benefits from being performed in an incubated environment. 1 Motorized microscopes that can function within incubators are commercially available 2-6 or described in published literature, 7-33 but may not have the necessary price point or features for widespread use in an academic research setting. While expensive research equipment is often acquired by institutions and shared among labs, equipment for time lapse imaging within an incubator over months-long time scales is not something that can easily be shared.…”

Section: Introductionmentioning

confidence: 99%

“…Some labs with technical expertise have developed and published their own microscope systems. 7-33 Custom systems may not have the sophistication or image quality of a commercial system, but can be much cheaper and are modifiable for specific applications. While optical components remain expensive to both commercial and academic microscope developers, electronic and mechanical components can be obtained cheaply due to the vast economic scale of consumer electronics and home appliances, and the recent boom of open source 3D printers provides a wealth of options for gantry design and motor control.…”

Section: Introductionmentioning

confidence: 99%

“…Some low cost DIY microscope systems are designed for portability for point-of-care solutions to specific problems, such as cytometry, 7-9 imaging for diagnostics, 10-15 parasite classification, 11, 16, 17 agricultural field work, 18 electrophysiology, 19-21 or education. 22-27 Such systems provide useful guidance for designing microscopes, but make image quality sacrifices in order to meet portability and cost requirements.…”

Section: Introductionmentioning

confidence: 99%

“…While optical components remain expensive to both commercial and academic microscope developers, electronic and mechanical components can be obtained cheaply due to the vast economic scale of consumer electronics and home appliances, and the recent boom of open source 3D printers provides a wealth of options for gantry design and motor control. Some low cost DIY microscope systems are designed for portability for point-of-care solutions to specific problems, such as cytometry, [7][8][9] imaging for diagnostics, [10][11][12][13][14][15] parasite classification, 11,16,17 agricultural field work, 18 electrophysiology, [19][20][21] or education. [22][23][24][25][26][27] Such systems provide useful guidance for designing microscopes, but make image quality sacrifices in order to meet portability and cost requirements.…”

Section: Introductionmentioning

confidence: 99%

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Automated Stimulation and Long-Term Remote Monitoring of Multi-Plex Microfluidic Organoid Chips

Sachs,

Costa

2023

Preprint

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Developing a novel microfluidic organoid system required many experiments and iterations due to lack of knowledge about the relevant developmental biology. Collecting data on the developing organoids quickly escalated into a bottleneck as high throughput and long term culture resulted in a rapidly increasing number of specimens being observed. Commercially available automated microscope systems exist, but were either too expensive or not appropriate, and could not be modified. To satisfy the increasing need for automated data collection, a custom robotic system was developed to collect data from within a standard incubator. An X-Y belt driven gantry was designed with an architecture chosen to balance high accuracy, low cost, speed, and range of motion. Focus control was implemented with dual miniature leadscrews. A linear sliding mechanism was used to switch between two microscope objectives. 3D printed chip attachments were designed to implement illuminators for bright field imaging, and electrodes for stimulating the cardiac organoids. A fluorescent filter block was designed using a 3D printed piece to hold optical components, and a multi-band filter set that allowed for three color fluorescence without moving parts. A pulley driven tilting stage gravitationally biased the organoids during development. In order to ensure accurate image collection despite the inevitable position shifting of the chips, an image processing pipeline was developed for locating organoids using geometrical microfluidic chip features. The resulting robotic system automated imaging data collection on organoids and electrical and mechanical stimulation, in addition to being modifiable for future projects.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%