3D-printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect

Construction of a stereocomplex between poly(d-lactide) grafted hydroxyapatite and poly(l-lactide): toward a bioactive composite scaffold with enhanced interfacial bonding

“…According to some studies, polymer/HAP scaffolds were able to induce the formation of the apatite layer. 48,49 During the process, the HAP surface would firstly be dissolved, and the dissolved Ca 2+ and PO 4 3- released into the SBF solution could increase the local concentrations of Ca 2+ , HPO 4 2− and PO 4 3− ions near the HAP surface. Afterwards, nucleation sites were formed on the HAP surface when the concentrations of calcium and phosphorus ions reached the threshold of nucleation.…”

Injectable click-crosslinked hydrogel containing resveratrol to improve the therapeutic effect in triple negative breast cancer

“…Several types of hydrogels have been widely utilized for the intratumoral delivery of Res [ 32 , 39 , 40 ]. Several groups have reported the good biocompatibility of crosslinked HA using carbodiimide series, (metha)acrylate series, diglycidyl ether, etc [ [41] , [42] , [43] ]. Among them, click-crosslinkable hydrogels remain in liquid form prior to injection but solidify through click-crosslinking during injection, allowing for the generation of a minimally invasive drug depot.…”

3D-printed polymeric scaffolds with optimized architecture to repair a sheep metatarsal critical-size bone defect

“…As a result, the BMP-2 dose should be carefully adapted to ensure an optimal bone regeneration without side effects. In this study, a BMP-2 dose of ~80 g/cm 3 of defect was targeted, based on our previous study on minipig mandible [53] where an efficient bone regeneration was found for this dose. BMP-2 incorporation in the 3D scaffolds was found to be higher in the present study compared to the previous one, with BMP-2 loaded dose of ~120 g/cm 3 of defect.…”

“…For BMP-2 incorporation in 3D experiments, the BMP-2 concentration varied between 10 and 50 g/mL, named hereafter BMP10, BMP30, and BMP50. For bone regeneration experiments, BMP-2 was post-loaded in the film at a targeted dose of ~500 g/total volume of defect, based on our previous study led on minipigs [53]. We took into account the fact that scaffolds had different surfaces and volumes and adapted the BMP-2 concentration in the loading solution to achieve the targeted BMP-2 dose.…”

“…To image the film deposited onto the 3D scaffolds, the last deposited layer was labeled with FITC (PLL FITC ) [34,53]. The scaffolds were then stored in 0.15 M NaCl until imaging using a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective.…”