3D printing microporous scaffolds from modular bioinks containing sacrificial, cell-encapsulating microgels

Seymour, Alexis J.; Kilian, David; Navarro, Renato S.; Hull, Sarah M.; Heilshorn, Sarah C.

doi:10.1039/d3bm00721a

Cited by 4 publications

(3 citation statements)

References 76 publications

(231 reference statements)

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“…The uniformity of pore structure in scaffolds played a pivotal role in facilitating cell infiltration and migration, nutrient transport, oxygen diffusion, and the expulsion of metabolic wastes. Furthermore, a more homogeneous longitudinal structure helped prevent the formation of discontinuities during the fabrication of intricate scaffolds, thus ensuring the integrity and functionality of the scaffold. , …”

Section: Results and Discussionmentioning

confidence: 99%

“…Furthermore, a more homogeneous longitudinal structure helped prevent the formation of discontinuities during the fabrication of intricate scaffolds, thus ensuring the integrity and functionality of the scaffold. 50,51 Enhancing Printing Resolution and Structural Fidelity through Rnps-Enriched Bioinks. To demonstrate the capability of Rnps in improving printing resolution and pattern fidelity, the lateral resolution of the prints was first evaluated by printing a spoke-like pattern, which consisted of 20 strips of 100 μm-wide arms as reported by Sun et al 21 (Figure 4A).…”

Section: Synthesis and Characterization Of Rnpsmentioning

confidence: 99%

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Efficient Light-Based Bioprinting via Rutin Nanoparticle Photoinhibitor for Advanced Biomedical Applications

Li,

Dai

et al. 2024

ACS Nano

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Digital light processing (DLP) bioprinting, known for its high resolution and speed, enables the precise spatial arrangement of biomaterials and has become integral to advancing tissue engineering and regenerative medicine. Nevertheless, inherent light scattering presents significant challenges to the fidelity of the manufactured structures. Herein, we introduce a photoinhibition strategy based on Rutin nanoparticles (Rnps), attenuating the scattering effect through concurrent photoabsorption and free radical reaction. Compared to the widely utilized biocompatible photoabsorber tartrazine (Tar), Rnps-infused bioink enhanced printing speed (1.9×), interlayer homogeneity (58% less overexposure), resolution (38.3% improvement), and print tolerance (3× high-precision range) to minimize trial-and-error. The biocompatible and antioxidative Rnps significantly improved cytocompatibility and exhibited resistance to oxidative stressinduced damage in printed constructs, as demonstrated with human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs). The related properties of Rnps facilitate the facile fabrication of multimaterial, heterogeneous, and cell-laden biomimetic constructs with intricate structures. The developed photoinhibitor, with its profound adaptability, promises wide biomedical applications tailored to specific biological requirements.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Synthesis and Characterization Of Rnpsmentioning

confidence: 99%

Efficient Light-Based Bioprinting via Rutin Nanoparticle Photoinhibitor for Advanced Biomedical Applications

Li,

Dai

et al. 2024

ACS Nano

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“…27–29 In addition to the advantages of bulk hydrogels, the scaffolds formed by micro and granular hydrogel have larger specific surface area and porosity. 30 Therefore, we hypothesized that the deposited microgel as microstructured coatings on electrospun membranes was expected to endow cell-instructive hydrophilic interfaces and also topological morphologies for cell adhesion. Additionally, to further improve the biological activity in regulating cell proliferation and differentiation, abdominal wall dECM was prepared and introduced into the microgel, which contains many cells binding site and is rich in active factors ( e.g.…”

Section: Introductionmentioning

confidence: 99%

Bioactive microgel-coated electrospun membrane with cell-instructive interfaces and topology for abdominal wall defect repair

Xing,

Gao,

Huangfu

et al. 2024

Biomater. Sci.

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3D Biofabrication of Microporous Hydrogels for Tissue Engineering

Liu,

Wu,

Luo

et al. 2024

Adv Healthcare Materials

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Microporous hydrogels have been utilized in an unprecedented manner in the last few decades, combining materials science, biology, and medicine. Their microporous structure makes them suitable for wide applications, especially as cell carriers in tissue engineering and regenerative medicine. Microporous hydrogel scaffolds provide spatial and platform support for cell growth and proliferation, which can promote cell growth, migration, and differentiation, influencing tissue repair and regeneration. This review gives an overview of recent developments in the fabrication techniques and applications of microporous hydrogels. The fabrication of microporous hydrogels can be classified into two distinct categories: fabrication of non‐injectable microporous hydrogels including freeze‐drying microporous method, two‐phase sacrificial strategy, 3D biofabrication technology, etc., and fabrication of injectable microporous hydrogels mainly including microgel assembly. Then, the biomedical applications of microporous hydrogels in cell carriers for tissue engineering, including but not limited to bone regeneration, nerve regeneration, vascular regeneration, and muscle regeneration are emphasized. Additionally, the ongoing and foreseeable applications and current limitations of microporous hydrogels in biomedical engineering are illustrated. Through stimulating innovative ideas, the present review paves new avenues for expanding the application of microporous hydrogels in tissue engineering.

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3D printing microporous scaffolds from modular bioinks containing sacrificial, cell-encapsulating microgels

Abstract: Blending sacrificial, cell-laden microgels with structural, UV-crosslinkable microgels produces a family of modular bioinks with tunable void fractions that influence cellular morphology while maintaining a depth-independent cell distribution.

Cited by 4 publications

References 76 publications

Efficient Light-Based Bioprinting via Rutin Nanoparticle Photoinhibitor for Advanced Biomedical Applications

Efficient Light-Based Bioprinting via Rutin Nanoparticle Photoinhibitor for Advanced Biomedical Applications

Bioactive microgel-coated electrospun membrane with cell-instructive interfaces and topology for abdominal wall defect repair

3D Biofabrication of Microporous Hydrogels for Tissue Engineering

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