3D Printing of Tissue Engineered Constructs for In Vitro Modeling of Disease Progression and Drug Screening

Vanderburgh, Joseph P.; Sterling, Julie A.; Guelcher, Scott A.

doi:10.1007/s10439-016-1640-4

Cited by 151 publications

(119 citation statements)

References 92 publications

(107 reference statements)

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“…Multi-matrix material bioinks are being developed meeting each structural, biologic and regulatory requirement [39] . Multimodal printers can deposit with high speed, mass and resolution [40] . Novel algorithms and software package guide the deposition of neovessels of various sources printed into synthetic networks designed to mature into a contiguous network of arterioles, capillaries, and venules.…”

Section: Resultsmentioning

confidence: 99%

“…Table 1. Lists of common bioink and 3DBP culture media component ingredients [7,19,20] Structural matrix elements Cell culture elements …”

Section: Matrix Scaffold or Ecmmentioning

confidence: 99%

“…These include the use of endothelial cells, both macrovascular and microvascular, endothelial progenitor cells (EPCs), perivascular cell precursors, mesenchymal and hematopoietic stem cells (MSCs and HSCs), and smooth muscle cells incorporated into the construct either alone or in combination. Adipose stromal vascular fraction (SVF) cells show particularly robust vasculogenic activity, perhaps because all of the cell types necessary to forming microvasculatures are present within the isolate [20,29] . Angiogenesis-based strategies include pre-packaging endothelial cells in clusters or aggregates, from which neovessels sprout [30] , or the use of intact microvessel fragments as a source of parent microvessels from which neovessels arise via angiogenesis [28] .…”

Section: Angiogenesis and Vasculogenesismentioning

confidence: 99%

See 2 more Smart Citations

Digital biomanufacturing supporting vascularization in 3D bioprinting

Whitford¹,

Hoying²

2017

ijb

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Synergies in bioprinting are appearing from individual researchers focusing on divergent aspects of the technology. Many are now evolving from simple mono-dimensional operations to model-controlled multi-material, interpenetrating networks using multi-modal deposition techniques. Bioinks are being designed to address numerous critical process parameters. Both the cellular constructs and architectural design for the necessary vascular component in digitally biomanufactured tissue constructs are being addressed. Advances are occurring from the topology of the circuits to the source of the of the biological microvessel components. Instruments monitoring and control of these activates are becoming interconnected. More and higher quality data are being collected and analysis is becoming richer. Information management and model generation is now describing a "process network." This is promising; more efficient use of both locally and imported raw data supporting accelerated strategic as well as tactical decision making. This allows real time optimization of the immediate bioprinting bioprocess based on such high value criteria as instantaneous progress assessment and comparison to previous activities. Finally, operations up-and down-stream of the deposition are being included in a supervisory enterprise control.

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Section: Resultsmentioning

confidence: 99%

“…Table 1. Lists of common bioink and 3DBP culture media component ingredients [7,19,20] Structural matrix elements Cell culture elements …”

Section: Matrix Scaffold or Ecmmentioning

confidence: 99%

Section: Angiogenesis and Vasculogenesismentioning

confidence: 99%

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Digital biomanufacturing supporting vascularization in 3D bioprinting

Whitford¹,

Hoying²

2017

ijb

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“…This review and the research articles presented here cover a wide range of applications, ranging from cardiovascular 19 to orthopedic, 9,10,58 craniofacial, 50 and drug screening. 71 As a prelude to this special issue, we decided to write an extended editorial and briefly review the most important trends in application of AM to healthcare, thereby setting the stage for what appears in the rest of the issue. In that sense, this editorial might be seen as a ''review of reviews,'' where we do not try to engage in the details of various areas of research but rather to sketch the bigger picture through clear examples, reference to the papers appearing in this special issue, as well as to the other essential literature.…”

Section: Introductionmentioning

confidence: 99%

Additive Manufacturing of Biomaterials, Tissues, and Organs

2016

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Abstract-The introduction of additive manufacturing (AM), often referred to as three-dimensional (3D) printing, has initiated what some believe to be a manufacturing revolution, and has expedited the development of the field of biofabrication. Moreover, recent advances in AM have facilitated further development of patient-specific healthcare solutions. Customization of many healthcare products and services, such as implants, drug delivery devices, medical instruments, prosthetics, and in vitro models, would have been extremely challenging-if not impossible-without AM technologies. The current special issue of the Annals of Biomedical Engineering presents the latest trends in application of AM techniques to healthcare-related areas of research. As a prelude to this special issue, we review here the most important areas of biomedical research and clinical practice that have benefited from recent developments in additive manufacturing techniques. This editorial, therefore, aims to sketch the research landscape within which the other contributions of the special issue can be better understood and positioned. In what follows, we briefly review the application of additive manufacturing techniques in studies addressing biomaterials, (re)generation of tissues and organs, disease models, drug delivery systems, implants, medical instruments, prosthetics, orthotics, and AM objects used for medical visualization and communication.

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“…Besides such neotissue engineering for potential clinical use, other applications of biomimetic OC TE include formation of the OC tissues to be used as high fidelity disease/healing models and as in vitro models for drug toxicity/efficacy evaluation [1,2].…”

Section: Introductionmentioning

confidence: 99%

Mimetic Hierarchical Approaches for Osteochondral Tissue Engineering

Gadjanski

2018

Osteochondral Tissue Engineering

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In order to engineer biomimetic osteochondral (OC) construct, it is necessary to address both the cartilage and bone phase of the construct, as well as the interface between them, in effect mimicking the developmental processes when generating hierarchical scaffolds that show gradual changes of physical and mechanical properties, ideally complemented with the biochemical gradients. There are several components whose characteristics need to be taken into account in such biomimetic approach, including cells, scaffolds, bioreactors as well as various developmental processes such as mesenchymal condensation and vascularization, that need to be stimulated through the use of growth factors, mechanical stimulation, purinergic signaling, low oxygen conditioning, and immunomodulation. This chapter gives overview of these biomimetic OC system components, including the OC interface, as well as various methods of fabrication utilized in OC biomimetic tissue engineering (TE) of gradient scaffolds. Special attention is given to addressing the issue of achieving clinical size, anatomically shaped constructs. Besides such neotissue engineering for potential clinical use, other applications of biomimetic OC TE including formation of the OC tissues to be used as high-fidelity disease/ healing models and as in vitro models for drug toxicity/efficacy evaluation are covered. Highlights Biomimetic OC TE uses "smart" scaffolds able to locally regulate cell phenotypes and dual-flow bioreactors for two sets of conditions for cartilage/ bone Protocols for hierarchical OC grafts engineering should entail mesenchymal condensation for cartilage and vascular component for bone Immunomodulation, low oxygen tension, purinergic signaling, time dependence of stimuli application are important aspects to consider in biomimetic OC TE Mimetic Hierarchical Approaches for Osteochondral Tissue Engineering Ivana GadjanskiAbstract In order to engineer biomimetic osteochondral (OC) construct, it is necessary to address both the cartilage and bone phase of the construct, as well as the interface between them, in effect mimicking the developmental processes when generating hierarchical scaffolds that show gradual changes of physical and mechanical properties, ideally complemented with the biochemical gradients. There are several components whose characteristics need to be taken into account in such biomimetic approach, including cells, scaffolds, bioreactors as well as various developmental processes such as mesenchymal condensation and vascularization, that need to be stimulated through the use of growth factors, mechanical stimulation, purinergic signaling, low oxygen conditioning, and immunomodulation. This chapter gives overview of these biomimetic OC system components, including the OC interface, as well as various methods of fabrication utilized in OC biomimetic tissue engineering (TE) of gradient scaffolds. Special attention is given to addressing the issue of achieving clinical size, anatomically shaped constructs. Besides such neotissue en...

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3D Printing of Tissue Engineered Constructs for In Vitro Modeling of Disease Progression and Drug Screening

Cited by 151 publications

References 92 publications

Digital biomanufacturing supporting vascularization in 3D bioprinting

Digital biomanufacturing supporting vascularization in 3D bioprinting

Additive Manufacturing of Biomaterials, Tissues, and Organs

Mimetic Hierarchical Approaches for Osteochondral Tissue Engineering

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