3D scaffolds prepared from acylated chitosan applicable in skin regeneration – synthesis and characterization

Radwan-Pragłowska, Julia; Piątkowski, Marek; Janus, Łukasz; Bogdał, Dariusz; Matýsek, Dalibor; Čablík, Vladimír

doi:10.1080/1023666x.2018.1553348

Cited by 5 publications

(13 citation statements)

References 14 publications

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“…Finally, bands at 2924 cm −1 and 2873 cm −1 corresponds to -CH-and -CH 2 -groups. The spectrum of the modified chitosan shows bands of significantly increased intensity at 2921 cm −1 and 2856 cm −1 , which proves acyl chains incorporation [28]. A new band at 1750 cm −1 can be noticed, which means that the acylation reaction occurred due to the formation of ester bonds between free hydroxyl groups of chitosan and carboxyl groups.…”

Section: Phenotype Studymentioning

confidence: 86%

“…Chitosan (CS) acylation proceeded according to a previously described method using fatty acid chloride as an acylating agent [28]. Briefly, 0.5 g of the chitosan was placed in an acetone/TEA solution and irradiated for 30 min in a microwave reactor.…”

Section: Chitosan Acylationmentioning

confidence: 99%

“…Figure 3c shows an SEM microphotograph of the acylated chitosan layer. It is known that, for new tissue formation, another important property of the material is its porosity, which enables new blood vessel formation and microcirculation development [27,28]. It can be noticed that the chitosan part of the scaffold is highly porous, and pores edges have a petal-like shape.…”

Section: Morphology Analysismentioning

confidence: 99%

“…There are numerous studies showing that the application of external stimuli improves various cell behaviors and accelerates tissue regeneration among which direct current stimulation can be named [28,[33][34][35][36][37][38][39]. The use of DC has been proven to improve the proliferation of various cell types including L929.…”

Section: Electrical Properties Of the Conductive Layermentioning

confidence: 99%

“…Another method is the chemical modification of the polymeric structure [24]. It has been shown that the acylation of chitosan using fatty acid chlorides improves its mechanical properties and in vitro fibroblasts behavior [28].…”

Section: Introductionmentioning

confidence: 99%

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Hybrid Bilayer PLA/Chitosan Nanofibrous Scaffolds Doped with ZnO, Fe3O4, and Au Nanoparticles with Bioactive Properties for Skin Tissue Engineering

et al. 2020

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Burns affect almost half a million of Americans annually. In the case of full-thickness skin injuries, treatment requires a transplant. The development of bioactive materials that promote damaged tissue regeneration constitutes a great alternative to autografts. For this reason, special attention is focused on three-dimensional scaffolds that are non-toxic to skin cells and can mimic the extracellular matrix, which is mainly composed of nanofibrous proteins. Electrospinning, which enables the preparation of nanofibers, is a powerful tool in the field of biomaterials. In this work, novel hybrid poly (lactic acid)/chitosan biomaterials functionalized with three types of nanoparticles (NPs) were successfully developed. ZnO, Fe3O4, and Au NPs were investigated over their morphology by TEM method. The top layer was obtained from PLA nanofibers, while the bottom layer was prepared from acylated chitosan. The layers were studied over their morphology by the SEM method and their chemical structure by FT-IR. To verify their potential in burn wound treatment, the scaffolds’ susceptibility to biodegradation as well as moisture permeability were calculated. Also, biomaterials conductivity was determined in terms of electrostimulation. Finally, cytotoxicity tests were carried out by XTT assay and morphology analysis using both fibroblasts cell line and primary cells. The hybrid nanofibrous scaffolds displayed a great potential in tissue engineering.

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Section: Phenotype Studymentioning

confidence: 86%

Section: Chitosan Acylationmentioning

confidence: 99%

Section: Morphology Analysismentioning

confidence: 99%

Section: Electrical Properties Of the Conductive Layermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hybrid Bilayer PLA/Chitosan Nanofibrous Scaffolds Doped with ZnO, Fe3O4, and Au Nanoparticles with Bioactive Properties for Skin Tissue Engineering

et al. 2020

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Stem Cell-Based Tissue Engineering for the Treatment of Burn Wounds: A Systematic Review of Preclinical Studies

Lukomskyj

Rao

Yan

et al. 2022

Stem Cell Rev and Rep

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Burn wounds are a devastating type of skin injury leading to severe impacts on both patients and the healthcare system. Current treatment methods are far from ideal, driving the need for tissue engineered solutions. Among various approaches, stem cell-based strategies are promising candidates for improving the treatment of burn wounds. A thorough search of the Embase, Medline, Scopus, and Web of Science databases was conducted to retrieve original research studies on stem cell-based tissue engineering treatments tested in preclinical models of burn wounds, published between January 2009 and June 2021. Of the 347 articles retrieved from the initial database search, 33 were eligible for inclusion in this review. The majority of studies used murine models with a xenogeneic graft, while a few used the porcine model. Thermal burn was the most commonly induced injury type, followed by surgical wound, and less commonly radiation burn. Most studies applied stem cell treatment immediately post-burn, with final endpoints ranging from 7 to 90 days. Mesenchymal stromal cells (MSCs) were the most common stem cell type used in the included studies. Stem cells from a variety of sources were used, most commonly from adipose tissue, bone marrow or umbilical cord, in conjunction with an extensive range of biomaterial scaffolds to treat the skin wounds. Overall, the studies showed favourable results of skin wound repair in animal models when stem cell-based tissue engineering treatments were applied, suggesting that such strategies hold promise as an improved therapy for burn wounds. Graphical abstract

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Process optimization for extraction of avian eggshell membrane derived collagen for tissue engineering applications

Aggarwal

Sah

2022

Journal of Polymer Engineering

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The avian eggshell membranes’ composition depicts close resemblance with the extracellular matrix of the cells, and therefore being widely employed as potential biomaterials for tissue engineering applications. However, the optimization of process conditions for collagen extraction, the main constituent of eggshell membranes is still challenging. In the present study, extraction of collagen was performed by an enzymatic method optimized through the one-factor-at-a-time (OFAT) technique for three parameters viz. pepsin concentration, treatment time and pH. The process optimization resulted in the maximum yield of 56% collagen with 350 U/mg pepsin concentration at pH 3 treated for 9 days, not reported yet. The collagen extraction was confirmed by OD at 232 nm; and its viscoelasticity behaviour at pH 5. The physico–chemical characterization of extracted collagen with FESEM, ATR-FTIR, surface roughness analysis and contact angle measurement revealed the morphological and topological alteration during the collagen extraction. The process optimization and characterization of eggshell membrane derived collagen can aid in the significant biomaterials development for tissue regeneration.

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3D scaffolds prepared from acylated chitosan applicable in skin regeneration – synthesis and characterization

Cited by 5 publications

References 14 publications

Hybrid Bilayer PLA/Chitosan Nanofibrous Scaffolds Doped with ZnO, Fe3O4, and Au Nanoparticles with Bioactive Properties for Skin Tissue Engineering

Hybrid Bilayer PLA/Chitosan Nanofibrous Scaffolds Doped with ZnO, Fe3O4, and Au Nanoparticles with Bioactive Properties for Skin Tissue Engineering

Stem Cell-Based Tissue Engineering for the Treatment of Burn Wounds: A Systematic Review of Preclinical Studies

Process optimization for extraction of avian eggshell membrane derived collagen for tissue engineering applications

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