3D Visualization of Calcified and Non-calcified Molluscan Tissues Using Computed Tomography

Sasaki, Takenori; Maekawa, Yu; Takeda, Yusuke; Atsushiba, Maki; Chen, Chong; Noshita, Koji; Uesugi, Kentaro; Hoshino, Masato

doi:10.1007/978-981-13-1002-7_9

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…charge-coupled device camera. Samples were rotated through 180° yielding 1860 images with effective pixel sizes of 6.5 μm (Chen et al 2018b, Sasaki et al 2018). The resulting images were processed in Adobe Photoshop CC for contrast enhancement and then imported into Amira v5.3.3 (FEI Visualization Sciences Group).…”

Section: Methodsmentioning

confidence: 99%

From wood to vent: first cocculinid limpet associated with hydrothermal activity discovered in the Weddell Sea

Chen

Linse

2020

Antarctic Science

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Lush ‘oases’ of life seen in chemosynthetic ecosystems such as hot vents and cold seeps represent rare, localized exceptions to the generally oligotrophic deep ocean floor. Organic falls, best known from sunken wood and whale carcasses, are additional sources of such oases. Kemp Caldera (59°42'S, 28°20'W) in the Weddell Sea exhibits active hydrothermal vents and a natural whale fall in close proximity, where an undescribed cocculinid limpet was found living in both types of chemosynthetic habitats. This represents the first member of the gastropod order Cocculinida discovered from hot vents, and also the first record from the Southern Ocean. Here, we applied an integrative taxonomy framework incorporating traditional dissection, electron microscopy, genetic sequencing and 3D anatomical reconstruction through synchrotron computed tomography in order to characterize this species. Together, our data revealed an unusual member of the genus Cocculina with a highly modified radula for feeding on bacterial film, described herein as Cocculina enigmadonta n. sp. Its phylogenetically derived position within the largely wood-inhabiting Cocculina indicates that it probably evolved from an ancestor adapted to living on sunken wood, providing a compelling case of the ‘stepping stone’ evolutionary trajectory from organic falls to seeps and vents.

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Section: Methodsmentioning

confidence: 99%

From wood to vent: first cocculinid limpet associated with hydrothermal activity discovered in the Weddell Sea

Chen

Linse

2020

Antarctic Science

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“…Martins (1996) [14] (178). Inner shell walls partly resorbed in [17] and figs 9.2c-d in [64]. For anatomical data see Martins (1996) [14].…”

Section: Systematicsmentioning

confidence: 99%

The Origin of the Mangrove and Saltmarsh Snail Ellobium (Eupulmonata, Ellobiidae)

Harzhauser

Pacaud²,

Landau

2023

Taxonomy

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The pulmonate gastropod genus Ellobium has its greatest diversity in the modern Indo-West Pacific Region (IWP). Its origin, however, is traced to the Early Oligocene of the Northeastern Atlantic and the Western Tethys Region. Two Ellobium species are documented from the Rupelian of France and Italy and a new species is recorded from the Chattian of Hungary: Ellobium kerwaensis nov. sp. The first records in the IWP are known from the Early Miocene, suggesting an eastward range expansion of the genus around the Oligocene/Miocene boundary, when Ellobium became extinct in the European seas. Extant Ellobium species are bound to habitats above the high tide line in salt marshes and mangroves. Comparable environmental requirements are expected for the fossil congeners. Ellobium may derive from Eocene ancestors, such as the Bartonian Eoellobium heberti from the Northeastern Atlantic. Eoellobium is introduced in this paper as a new genus.

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“…The rehydrated limpet was stained for 48 h in 1% iodine solution prior to the CT scan. The stained specimen was washed twice in MilliQ water and placed inside an X-ray transparent polystyrene container filled with MilliQ water, as per published methods [28]. The specimen was scanned wet using a ScanXmate-D160TSS105 (Comscantecno, Japan) commercial μ-CT at 80 kV/51 µA with the resolution of 3.150 µm per pixel, at 992 pixels by 992 pixels per slice.…”

Section: Three-dimensional Anatomical Reconstructionmentioning

confidence: 99%

Integrative taxonomy of a new cocculinid limpet dominating the Aurora Vent Field in the central Arctic ocean

et al. 2022

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Deep-sea hydrothermal vents host lush chemosynthetic communities, dominated by endemic fauna that cannot live in other ecosystems. Despite over 500 active vents found worldwide, the Arctic has remained a little-studied piece of vent biogeography. Though located as early as 2001, the faunal communities of the Aurora Vent Field on the ultra-slow spreading Gakkel Ridge remained unsampled until recently, owing to difficulties with sampling on complex topography below permanent ice. Here, we report an unusual cocculinid limpet abundant on inactive chimneys in Aurora (3883–3884 m depth), describing it as Cocculina aurora n. sp. using an integrative approach combining traditional dissection, electron microscopy, molecular phylogeny, and three-dimensional anatomical reconstruction. Gross anatomy of the new species was typical for Cocculina , but it has a unique radula with broad, multi-cuspid rachidian where the outermost lateral is reduced compared to typical cocculinids. A phylogenetic reconstruction using the mitochondrial COI gene also confirmed its placement in Cocculina . Only the second cocculinid found at vents following the description of the Antarctic Cocculina enigmadonta , this is currently the sole cocculinid restricted to vents. Our discovery adds to the evidence that Arctic vents host animal communities closely associated with wood falls and distinct from other parts of the world.

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3D Visualization of Calcified and Non-calcified Molluscan Tissues Using Computed Tomography

Cited by 4 publications

References 18 publications

From wood to vent: first cocculinid limpet associated with hydrothermal activity discovered in the Weddell Sea

From wood to vent: first cocculinid limpet associated with hydrothermal activity discovered in the Weddell Sea

The Origin of the Mangrove and Saltmarsh Snail Ellobium (Eupulmonata, Ellobiidae)

Integrative taxonomy of a new cocculinid limpet dominating the Aurora Vent Field in the central Arctic ocean

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