3M™ Petrifilm™ Staph Express Count Plate Method for the Enumeration of Staphylococcus aureus in Selected Types of Meat, Seafood, and Poultry: Collaborative Study

McMahon, Wendy; Aleo, V; Schultz, Ann; Horter, Barbara L.; Lindberg, Kathryn

doi:10.1093/jaoac/86.5.947

Cited by 20 publications

(11 citation statements)

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“…Our results showed the low presence of S. aureus in chicken nugget samples (6.42%) that is in contrast with previous studies ( 7 , 24 ). Only three studies were performed concerning detection of S. aureus in poultry samples in Iran ( 12 - 14 ), which are in agreement with our results.…”

Section: Discussioncontrasting

confidence: 99%

Prevalence of Enterotoxigenic Staphylococcus aureus Isolated From Chicken Nugget in Iran

Madahi

Rostami

Rahimi

et al. 2014

Jundishapur J Microbiol

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Background:The enterotoxigenic Staphylococcus aureus is considered as one of the most important cause of food poisoning that manifests with gastroenteritis, diarrhea, and vomiting. Its complications usually occur when bacterial virulence genes are produced. The most important virulence factors are cell-associated components, exoenzymes, exotoxins, enterotoxins, and enterotoxin-like toxins.Objectives:The present study aimed to study the presence of S. aureus and its virulence factors in chicken nuggets in Iran.Materials and Methods:Totally, 420 chicken nuggets from five brands were collected from Isfahan and Chaharmahal-va-Bakhtiari provinces, Iran. Samples were cultured and the positive results were studied using ELISA and PCR for detection of classical staphylococcal enterotoxins and sea-sej virulence genes, respectively.Results:Results showed that 27 (6.42%) of 420 samples were contaminated with S. aureus with bacteria concentration between 6.1 × 103 to 8.4 × 101/mL. Totally, 33.33% of isolates produced SEA, 4.16% SEB, 12.50% SEC, 8.33% SED, 12.50% SEA + SEC, and 12.50% SEA + SED. The most commonly detected genes were sea (25%), sea + seg (8.33%), sec (12.50%), sea + sed (12.50%), and sea + sec + sej (12.50%).Conclusions:S. aureus can easily contaminate the chicken nugget and this contamination is usually associated with significant presences of virulence genes. Consumption of these nuggets certainly is associated with gastrointestinal diseases. Therefore, some food safety and quality standards should be applied and performed in most of the Iranian food units to control growth of S. aureus and its virulence factors.

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Section: Discussioncontrasting

confidence: 99%

Prevalence of Enterotoxigenic Staphylococcus aureus Isolated From Chicken Nugget in Iran

Madahi

Rostami

Rahimi

et al. 2014

Jundishapur J Microbiol

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“…To measure the S. aureus loads in the identified milk samples, we spread 1 mL of each milk sample on a Petrifilm Staph Express Count plate, which is known to be suitable for identifying mastitis pathogens (21)(22)(23), incubated the plates at 37 • C for 24 h, and then counted the colonies. The CFU/mL was calculated using the number of bacteria.…”

Section: Identification Of S Aureus In Milk By the Bacteriological Cmentioning

confidence: 99%

Rapid Staphylococcus aureus Detection From Clinical Mastitis Milk by Colloidal Gold Nanoparticle-Based Immunochromatographic Strips

et al. 2020

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Rapid diagnostic technologies for bovine mastitis caused by Staphylococcus aureus (S. aureus) are urgently needed. In the current study, we generated an anti-ribosomal protein-L7/L12 antibody to detect S. aureus and an anti-ribosomal protein-L7/L12 antibody-coated immune-chromatographic strip (ICS) test. Moreover, we determined the ability of the ICS test to detect S. aureus from milk samples collected from cows with clinical mastitis. The developed ICS reacted to S. aureus in a bacteria load-dependent manner with a detection limit of ∼10 4 CFU/mL. In the evaluation of possible cross-reactivity of the ICS test, six strains of coagulase-negative Staphylococci showed slightly positive reactions, although at a lower level; however, other bacteria were completely negative. Next, we investigated the sensitivity and specificity of the ICS test compared with the bacteriological culture method using milk samples from clinical bovine mastitis. The results of the experiments demonstrated that the ICS test had high sensitivity [100%, 95% confidence interval (CI): 91.3-100%] and specificity (91.9%, CI: 90.5-91.9%) compared with culture tests. In addition, the kappa statistic demonstrated that ICS tests showed substantial agreement (k = 0.77, CI: 0.66-0.87) with culture tests. Positive correlations were observed for the statistical analysis between S. aureus (nuc gene) copy numbers and ICS test scores in mastitic milk infected by S. aureus. Therefore, we assume that this new detection method using ICS may be useful as a highly sensitive S. aureus-screening method for the diagnosis of bovine mastitis. Our findings support the ongoing effort to develop an ICS method for bovine S. aureus-induced mastitis, which can contribute to the rapid diagnosis of this disease.

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“…For plasma irradiation time of more than 2 minutes, we mixed the mulberry leaves' fine powder for about 5 seconds every 2 minutes since the powder diameter is very small at 0.5 mm or smaller, so that the plasma will be irradiated uniformly over the mulberry leaves' fine powder in the beaker. e number of general bacteria and coliform bacteria was counted by the 3M ™ Petrifilm ™ (3M Company) method [31] with 100-time diluted solution using 3M ™ Petrifilm ™ AC medium for general bacteria and 3M ™ Petrifilm ™ CC medium for coliform bacteria. Journal of Food Quality…”

Section: Methodsmentioning

confidence: 99%

Development of Decontamination Treatment Techniques for Dry Powder Foods by Atmospheric-Pressure Nonequilibrium DC Pulse Discharge Plasma Jet

Yuji

Nakabayashi

Kinoshita

et al. 2021

Journal of Food Quality

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Dry powder food ingredients imported to Japan contain large amounts of viable bacteria and coliform bacteria, and we need a simple, low-cost, dry nonthermal decontamination method without spoiling nutrients, color, fragrance, and flavor. In this study, it is shown that the decontamination performance against viable bacteria and coliform bacteria is proportional to the plasma irradiation time when OH and O3 radicals are incident on the dry powder food ingredients placed in an atmospheric-pressure nonequilibrium DC pulse discharge Ar + O2 mixture gas plasma jet. Our study revealed that there is a correlation between the plasma irradiation time and DC pulse frequency increase and the decontamination effect on the general bacterial count.

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3M™ Petrifilm™ Staph Express Count Plate Method for the Enumeration of Staphylococcus aureus in Selected Types of Meat, Seafood, and Poultry: Collaborative Study

Cited by 20 publications

References 0 publications

Prevalence of Enterotoxigenic Staphylococcus aureus Isolated From Chicken Nugget in Iran

Prevalence of Enterotoxigenic Staphylococcus aureus Isolated From Chicken Nugget in Iran

Rapid Staphylococcus aureus Detection From Clinical Mastitis Milk by Colloidal Gold Nanoparticle-Based Immunochromatographic Strips

Development of Decontamination Treatment Techniques for Dry Powder Foods by Atmospheric-Pressure Nonequilibrium DC Pulse Discharge Plasma Jet

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