3P143 Sequential analysis of connectin-like protein in acorn worm(10. Muscle,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Abstract

Connectin is the largest elastic protein that connects between Z-line and Mline of sarcomere and functions as a molecular spring of vertebrate striated muscles. In the upstream region of connectin gene, there are two genes for proteins which domain structures are closely related with that of connectin. Although RT-PCR experiments revealed that these two genes are expressed in striated muscle, their function remains unknown. In this study, we investigated the domain structures, isoforms, localizations and binding partners of these proteins in striated muscles. To know their physiological roles, we examined the effects of overexpression or knock-down of these proteins using cultured skeletal muscle cells. In this presentation, we introduce the result of our experiments. 3P140