3PT234 Hydrogen-bonding interactions between BChl-a and Trp residues in the light-harvestingl complexes from Thermochro-matium tepidum(The 50th Annual Meeting of the Biophysical Society of Japan)

Abstract

Thermochrornatium (Tch.) tepidum, exhibits enhanced thermal stability as compared with that ofthe mesophilic counterpart, At]ochrornatium (Alc.) vinosurn despite 82% of sequence homology between two species. Recent]y, it was proposed that several factors jnetuding numbers of hydrogen bonds, flexibility and eompactness ofthe structure enhance the thermal stabi]ity of Tch. tepidum Cyt c'. To understand more details of the thermostabilization, we focused on the amino acid residues at Dl31 or R129 pos{tion in the Cterminal region ofTch. tepidum Cyt c', and introduced single rnutation using the