3SDA-06 Development of a function of Pt(II)-diradical complex as a near-infrared absorbing probe(Cutting-Edge Optical Imaging Approaches and Raman Micro-Spectroscopy Pioneering Bio-Physics,Symposium,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Abstract

Absorption spectrum has been known as "molecular fingerprint". However, because of the short optical path length of cells, it is hard to measure absorption spectrum for individual cells. Here, we developed optical cavity enhanced light absorption spectroscopic microscopy for two-dimensional light absorption imaging. This microscopy enabled to detect absorption spectrum with only 10 μ m optical path length samples at subcellular spatial resolution, and image various cell types that showed the cellular diversity among not only different species but also identical cell types without any staining. Thus, our microscopy allows to measure the absorption spectra of biological samples, thereby detecting individuality of cells. 3SDA-04 光シート顕微鏡による生体まるごとイメージング Live imaging of whole organisms by light-sheet microscopy Shigenori Nonaka (NIBB)Light-sheet microscopy is a recently developed technology that uses thin sheet-shaped excitation light to illuminate the focal plane of a detection objective. This method is characterized by low bleaching and phototoxicity, deep penetration length, and high-speed image acquisition. These features are extremely suitable for live imaging of whole organisms of submillimeter scale. Here I show two examples that are first enabled by this microscopy: One is gastrulating mouse embryos, by which we revealed migration pattern of invaginated mesodermal cells. The other is high-speed 4D imaging of moving Amoeba proteus, that is enabled by moving the light-sheet and the detection objective instead of the specimen. 3SDA-05 小分子から生細胞まで-ラマン顕微鏡にできること- Small Molecules and Live Cells Characterized Using Raman Microscopes Shin-ichi Morita (Tohoku Univ.)It is now possible to measure the Raman spectrum of a single live cell. Proteins, nucleic acids, lipids, etc. are vibrated with different wavenumbers. These oscillators are interacted with the light, eventually detected as Raman scattering. Using Raman signals, it is capable to analyze the distribution of biomolecules. It is however difficult to distinguish Raman bands of similar molecules. Raman analysis is therefore ambiguous. To clarify this, we are interested in developing Raman probes such as alkyne tags for small molecules. Also, we have been interested in direct measurements of Raman spectra of live cells, because using Raman microscopes differentiation of live cells was distinguished. Recent results were discussed in the talk. 3SDA-06 近赤外吸収プローブとしての Pt(II)ジラジカル錯体の機能 開発 Development of a function of Pt(II)-diradical complex as a nearinfrared absorbing probe