4-Hydroxyderricin inhibits osteoclast formation and accelerates osteoblast differentiation

Abstract

4-Hydroxyderricin (4-HD) is a major polyphenol of Angelica keiskei (Japanese name Ashitaba), exhibiting anti-allergic, anti-diabetic, anti-oxidant, and antitumor effects. The present study was designed to evaluate the effects of 4-HD on bone formation and maintenance by using cultured osteoclasts and osteoblasts. 4-HD did not affect cell proliferation of stromal ST2 cells and preosteoblast MC3T3-E1 cells at concentrations of 1–10 μM. This compound inhibited the formation of multinucleated osteoclasts from mouse splenic cells, and we identified a molecular pathway of osteoclast differentiation mediated by 4-HD, which led to inhibition of the expression of receptor activator of nuclear factor-κB ligand and macrophage-colony stimulating factor in ST2 cells. By contrast, 4-HD enhanced indices of osteoblast differentiation, such as alkaline phosphatase activity and calcium deposition by osteoblastic MC3T3-E1 cells, at concentrations of 1–10 μM. Furthermore, we found that 4-HD at 1 μM attenuated H 2 O 2 levels in MC3T3-E1 cells. Our findings indicate that 4-HD may have critical effects on bone formation and maintenance.

Simultaneous Optimization of Ultrasound-Assisted Extraction for Flavonoids and Antioxidant Activity of Angelica keiskei Using Response Surface Methodology (RSM)

“…Recently, it has gained popularity as a functional or nutritional component used in beverages, daily chemicals and healthcare products, such as tea bags [10], cosmetics [11], capsules of dietary supplements [12], etc. Moreover, it has been reported to possess properties of anti-oxidative [13], anti-bacterial [14], anti-virus [15], anti-osteoporosis [16], anti-tumor [17], anti-hyperlipidemic [18], ameliorate inflammation [19] and prevention of metabolic syndrome [20]. Noticeably, these biological activities are documented and attributed to the presence of varieties of bioactive constituents in A. keiskei , such as flavonoids, coumarins, terpenoids, acetylenes, phenolics and other compounds [21].…”