4-Nonylphenol effects on rat testis and sertoli cells determined by spectrochemical techniques coupled with chemometric analysis

Duan, Peng; Liu, Bisen; Morais, Camilo L. M.; Zhao, Jing; Li, Xiandong; Tu, Jian; Yang, Weiyingxue; Chen, Chunling; Long, Manman; Feng, Xiaobing; Martin, Francis; Xiong, Chengliang

doi:10.1016/j.chemosphere.2018.11.086

Cited by 20 publications

(44 citation statements)

References 63 publications

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“…Based on the PCA‐LDA, each spectrum can be assigned scores for both LD1 and LD2. If these discriminant scores are delineated on the two‐dimensional (2D) scores plot, then the segregation between classes with their similarities/dissimilarities can be identified on the basis of the distance between clusters . The risk of overfitting is reduced in LDA by applying cross‐validation.…”

Section: Methodsmentioning

confidence: 99%

“…Intensity ratios of Raman peaks 1303 cm −1 /1267 cm −1 and 1654 cm −1 /1445 cm −1 could be used to evaluate the levels of saturated and unsaturated lipids , respectively. The unsaturated‐to‐saturated lipids ratio was obtained by calculating the intensity ratio of (1303 cm −1 /1267 cm −1 )/(1654 cm −1 /1445 cm −1 ).…”

Section: Methodsmentioning

confidence: 99%

“…In combination with chemometric methods, such as principal component analysis plus linear discriminant analysis (PCA‐LDA), FTIR and Raman spectroscopic analyses have recently shown great promise as a means to facilitate biomarker discovery, sample clustering and classification from spectral datasets . In particular, both FTIR and Raman spectroscopies have been successfully applied in the field of biological and clinical researches, such as environmental toxicology, epigenetics, pathology, laboratory diagnosis and cytology .…”

Section: Introductionmentioning

confidence: 99%

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Fourier transform infrared and Raman‐based biochemical profiling of different grades of pure foetal‐type hepatoblastoma

Duan

Yang

et al. 2019

Journal of Biophotonics

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The biomolecular events resulting from the progression of hepatoblastoma remain to be elucidated. Fourier‐transform infrared (FTIR) and Raman spectroscopies are capable of noninvasively and accurately capturing the biochemical properties of biological tissue from its pathological status. Our aim was to probe critial biomolecular changes of liver accompanying the progression of pure foetal hepatoblastoma (PFH) by FTIR and Raman spectroscopies. Herein, biochemical alterations were both evident in the FTIR spectra (regions of 3100‐2800 cm−1 and 1800‐900 cm−1) and the Raman spectra (region of 1800‐400 cm−1) among normal, borderline and malignant liver tissues. Compared with normal tissues, the ratios of protein‐to‐lipid, α‐helix‐to‐β‐sheet, RNA‐to‐DNA, CH3 methyl‐to‐CH2 methylene, glucose‐to‐phospholipids, and unsaturated‐to‐saturated lipids intensities were significantly higher in malignant tissues, while the ratios of RNA‐to‐Amide II, DNA‐to‐Amide II, glycogen‐to‐cholesterol and Amide I‐to‐Amide II intensities were remarkably lower. These biochemical alterations in the transition from normal to malignant have profound implications not only for cyto‐pathological classification but also for molecular understanding of PFH progression. The successive changes of the spectral characteristics have been shown to be consistent with the development of PFH, indicating that FTIR and Raman spectroscopies are excellent tools to interrogate the biochemical features of different grades of PFH.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fourier transform infrared and Raman‐based biochemical profiling of different grades of pure foetal‐type hepatoblastoma

Duan

Yang

et al. 2019

Journal of Biophotonics

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“…4-nonylphenol (NP) as a member of the endocrine disrupters is extensively used as nonionic surfactants in a variety of industrial and agricultural products. This disruptor causes various problems in the normal, hormonal function of animals or human beings [12, 13, 14, 15]. Therefore, developing novel methods for determination of NP and detection of its damage to DNA are highly demanded.…”

Section: Introductionmentioning

confidence: 99%

Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol

Ghanbari

Roshani

Goicoechea

et al. 2019

Heliyon

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In this work, a novel biosensor was fabricated for detection of DNA damage induced by 4-nonylphenol (NP) and also determination of NP. To achieve this goal, a glassy carbon electrode (GCE) was modified with chitosan (Chit), gold nanoparticles (Au NPs) and DNA-multiwalled carbon nanotubes (DNA-MWCNTs). Then, the DNA-MWCNTs/ Au NPs/Chit/GCE was incubated with methylene blue (MB) to obtain MB-DNA-MWCNTs/Au NPs/Chit/GCE in which MB was used as the redox indicator. The modifications applied to the GCE were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopic (EDS) and theoretical evidence. MB is a derivative of anthraquinone which can intercalate into double helix structure of DNA. By treating MB-DNA-MWCNTs/Au NPs/Chit/GCE with NP, a higher R ct was observed because the insertion of the NP may result in a more negative charge environment on the DNA surface which hinders accessibility of [Fe(CN) 6 ] 3-/4anion to the electrode surface. Change in the EIS response of the biosensor in the presence of NP was used to develop a novel system for monitoring the DNA damage induced by NP. The EIS technique was also used to develop a sensitive electroanalytical method for determination of NP.

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“…In classification applications, samples are assigned to groups based on their IR spectrochemical signature. This includes, for example, differentiation of brain tumour types (3), identification of neurodegenerative diseases (4), cervical cancer screening (5), endometrial and ovarian cancer identification (6), identification of prostate cancer tissue samples (7), differentiation of endometrial tissue regions (8), toxicology screening (9,10), and microbiologic studies involving fungi and virus identification (11)(12)(13). However, before model construction, a fundamental step is to split the spectral dataset into at least two subsets: training and test.…”

Section: Introductionmentioning

confidence: 99%

A computational protocol for sample selection in biological-derived infrared spectroscopy datasets using Morais-Lima-Martin (MLM) algorithm

Morais¹,

Lima²,

Martin³

2018

Protocol Exchange

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Infrared (IR) spectroscopy is a powerful analytical technique that can be applied to investigate a wide range of biological materials (e.g., biofluids, cells, tissues), where a specific biochemical signature is obtained representing the 'fingerprint' signal of the sample being analysed. This chemical information can be used as an input data for classification models in order to distinguish or predict samples groups based on computational algorithms. One fundamental step towards building such computational models is sample selection, where a fraction of the samples measured during an experiment are used for building the classifier, whereas the remaining ones are used for evaluating the model classification performance. This protocol shows how sample selection can be performed in a computational environment (MATLAB) by using a combination of Euclidian-distance calculation and random selection, named Morais-Lima-Martin (MLM) algorithm, as a previous step before building classification models in biological-derived IR datasets.

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4-Nonylphenol effects on rat testis and sertoli cells determined by spectrochemical techniques coupled with chemometric analysis

Abstract: CLoK Central Lancashire online Knowledge www.clok.uclan.ac.uk 4-Nonylphenol effects on rat testis and Sertoli cells determined by spectrochemical techniques coupled with chemometric analysis

Cited by 20 publications

References 63 publications

Fourier transform infrared and Raman‐based biochemical profiling of different grades of pure foetal‐type hepatoblastoma

Fourier transform infrared and Raman‐based biochemical profiling of different grades of pure foetal‐type hepatoblastoma

Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol

A computational protocol for sample selection in biological-derived infrared spectroscopy datasets using Morais-Lima-Martin (MLM) algorithm

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