4. Practical and Ethical Issues in Establishinga Collection of Normal Breast Tissue Biopsies—Part of the NOWACPost-Genome Cohort

Krum-Hansen, Sanda; Olsen, Karina Standahl

doi:10.18261/9788215041193-2020-04

Cited by 1 publication

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A detailed description of the recruitment proses and study population as well as ethical aspects of genetic research in healthy populations are presented and discussed in our previously published article. 13 Briefly, study participants were recruited through the national mammography screening program at the Breast Diagnostic Center at the University Hospital of North Norway (UNN), Tromsø, Norway, during the years 2010–2011. They were not referred due to pathological clinical findings or irregularities on previous mammograms but attended a scheduled routine mammogram.…”

Section: Methodsmentioning

confidence: 99%

Associations of breast cancer related exposures and gene expression profiles in normal breast tissue—The Norwegian Women and Cancer normal breast tissue study

et al. 2023

View full text Add to dashboard Cite

BackgroundNormal breast tissue is utilized in tissue‐based studies of breast carcinogenesis. While gene expression in breast tumor tissue is well explored, our knowledge of transcriptomic signatures in normal breast tissue is still incomplete. The aim of this study was to investigate variability of gene expression in a large sample of normal breast tissue biopsies, according to breast cancer related exposures (obesity, smoking, alcohol, hormone therapy, and parity).MethodsWe analyzed gene expression profiles from 311 normal breast tissue biopsies from cancer‐free, post‐menopausal women, using Illumina bead chip arrays. Principal component analysis and K‐means clustering was used for initial analysis of the dataset. The association of exposures and covariates with gene expression was determined using linear models for microarrays.ResultsHeterogeneity of the breast tissue and cell composition had the strongest influence on gene expression profiles. After adjusting for cell composition, obesity, smoking, and alcohol showed the highest numbers of associated genes and pathways, whereas hormone therapy and parity were associated with negligible gene expression differences.ConclusionOur results provide insight into associations between major exposures and gene expression profiles and provide an informative baseline for improved understanding of exposure‐related molecular events in normal breast tissue of cancer‐free, post‐menopausal women.

show abstract

Section: Methodsmentioning

confidence: 99%