In this study, the evaporation heat transfer characteristics of an intermediate temperature of R744 in a smooth horizontal tube, when operating as an indirect refrigeration system (IRS) among hybrid cascade refrigeration systems (HCRSs), are evaluated. Studies on the characteristics of intermediate-temperature evaporation heat transfer under the operating conditions of evaporators used in actual refrigeration systems, such as IRS, cascade refrigeration systems, and HCRS, used in supermarkets are lacking. Thus, this study provides basic data on the characteristics of evaporation heat transfer of R744 in the evaporators of refrigerators used in supermarkets. The tube employed to the evaporation experiment in this study was a horizontal smooth copper tube with a length of 8000 mm and an inner diameter of 11.46 mm. The experimental variables were measured over a wide range of mass flux of 200–500 kg/(m2·s), heat flux of 10–40 kW/m2, and saturation temperature of −40–0 °C. The main results are summarized as follows: (1) The application of Kandlikar’s correlation formula at an evaporation temperature of −20 °C in an IRS helps in a good prediction of the R744 evaporation heat transfer coefficient. (2) The pressure drop according to the heat and mass flux showed the same heat transfer coefficient trend, but the pressure drop at saturation temperature was different from the trend of heat transfer coefficient.