5-bromodeoxyuridine tablets with improved depot effect for analysis in vivo of sister-chromatid exchanges in bone-marrow and spermatogonial cells

King, M.-T.; Wild, D.; Gocke, Elmar; Eckhardt, K.

doi:10.1016/0165-1161(82)90009-7

Cited by 49 publications

(14 citation statements)

References 27 publications

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“…The results obtained in the present study are consistent with earlier results indicating a lower response of spermatogonia to SCE induction by some chemical mutagens [Allen et al, 1978;King et al, 1982;Palitti et al, 19821, and a very low basal SCE frequency [Allen and Latt, 1976;King et al, 1982;Morales-Ram'rez et al, 1984bl. These responses could be explained by the presence of barriers which restrict the movement of substances toward the microtubular lumen [Russell, 19901. However, because the presence of such cell barriers did not shield spermatogonia from gamma ray exposure, the lower induction of SCE by gamma ray in spermatogonia could be due to a much higher efficiency in DNA damage repair.…”

Section: Discussionsupporting

confidence: 95%

“…There is consistent evidence demonstrating that spermatogonia show a lower response to SCE induction by chemical mutagens [Allen et al, 1978;King et al, 1982;Palitti et al, 19821. This could be explained by a higher repair capacity, by a lower cell-specific inducibility of damage involved in SCE, or by the presence of physiological barriers that limit the movement of substances toward the germinal cells [Russell, 19901.…”

Section: Discussionmentioning

confidence: 93%

“…The DNA damage eliciting SCE is not known, but different possible causes have been proposed [Carrano et al, 1979;Cassel and Latt, 1980;Kano and Fujiwara, 1982;Ishii, 19811. However, many lines of evidence also suggest that DNA breaks are not involved [Fornace et al, 1980;Kinsella and Radman, 1980;MacRae et al, 1979;Morales-Ramirez et al, 1983;Shiraishi and Sandberg, 19791. There is consistent evidence demonstrating that spermatogonia show a lower response to SCE induction by chemical mutagens [Allen et al, 1978;King et al, 1982;Palitti et al, 19821, a fact which may be explained by the presence of physiological barriers that limit the movement of substances toward the germinal cells [Russell, 19901, by a higher efficiency in repair, or by fewer cell-specific zones in the genome in which SCE could occur.…”

Section: Introductionmentioning

confidence: 97%

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Comparison of sister chromatid exchange induction in murine germinal and somatic cells by gamma radiation exposure in vivo

Morales-Ramı́rez

Mendiola-Cruz

Vallarino-Kelly

et al. 1994

Environ and Mol Mutagen

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Sister chromatid exchange (SCE) induction by gamma rays was determined in spermatogonia irradiated before or after BrdU incorporation. Furthermore, the comparison of responses obtained in spermatogonia, bone marrow and salivary gland cells was carried out in the cells irradiated after BrdU incorporation, a condition which permits a higher SCE induction. Results indicate that gamma ray exposure of spermatogonia could induce a significant increase in SCE frequency with doses as low as 0.27 Gy, either before or after BrdU incorporation. However, the increase caused by radiation exposure after BrdU incorporation in spermatogonia was nearly three times lower than that obtained in both bone marrow and salivary gland cells. These data suggest that spermatogonia are either more efficient in repairing the gamma ray-induced lesions involved in SCE production or that these cells are less prone to the induction of such lesions.

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Section: Discussionsupporting

confidence: 95%

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 97%

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Comparison of sister chromatid exchange induction in murine germinal and somatic cells by gamma radiation exposure in vivo

Morales-Ramı́rez

Mendiola-Cruz

Vallarino-Kelly

et al. 1994

Environ and Mol Mutagen

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“…Cell proliferation and its degree can be evaluated using 5-bromo-2′-deoxyuridine (BrdU) which is incorporated into the newly synthesized DNA, during the S phase of the cell cycle [8]. BrdU is a thymine analogue which pairs with purine bases with different affi nity depending on its isoform, i.e.…”

Section: Introductionmentioning

confidence: 99%

“…Acta Veterinaria-Beograd 2017, 67 (1), [1][2][3][4][5][6][7][8][9][10] Authors' contributions JG and DM participated in the design of the study and performed the statistical analysis. NR conceived of the study, and participated in its design and coordination and helped to draft the manuscript.…”

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confidence: 99%

Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach

et al. 2017

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The study of cell proliferation is a useful tool in the fi elds of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2′-deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2′-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2′-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifi ce by fl at-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2′-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2′-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2′-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2′-deoxyuridine in vivo labeling provides a simple, effi cient, and reliable method for cell labeling, and at the same minimizes stress to animals.

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In VivoGenotoxicity Assays

Thybaud

2010

Cancer Risk Assessment

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5-bromodeoxyuridine tablets with improved depot effect for analysis in vivo of sister-chromatid exchanges in bone-marrow and spermatogonial cells

Cited by 49 publications

References 27 publications

Comparison of sister chromatid exchange induction in murine germinal and somatic cells by gamma radiation exposure in vivo

Comparison of sister chromatid exchange induction in murine germinal and somatic cells by gamma radiation exposure in vivo

Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach

In VivoGenotoxicity Assays

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