5-Hydroxymethylfurfural, an antioxidant agent from Alpinia oxyphylla Miq. improves cognitive impairment in Aβ1–42 mouse model of Alzheimer's disease

Liu, Aijing; Zhao, Xu; Li, Huan; Liu, Zhi; Liu, Bing; Mao, Xin; Guo, Lin; Bi, Kaishun; Jia, Ying

doi:10.1016/j.intimp.2014.10.028

Cited by 75 publications

(56 citation statements)

References 31 publications

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“…Shu-Di can also inhibit p53, p21 and p16 expression, protect brain tissue from oxidation and delay brain senescence in D-galactose-induced senile rats (21,22). Catalpol, a compound in Shu-Di, has been demonstrated to attenuate the Aβ 1-42 -mediated neurotoxicity, improve the endocrine function and alleviate structural damage of the hypothalamus in Aβ [25][26][27][28][29][30][31][32][33][34][35] -induced AD rats (23,24).…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, lyophilized Aβ [25][26][27][28][29][30][31][32][33][34][35] was dissolved in RPMI 1640 medium and incubated at 37˚C with constant oscillation for 3 days in order to induce aggregation. The aggregated Aβ [25][26][27][28][29][30][31][32][33][34][35] was then diluted to 100 µg/ml (100 µM) and stored at -20˚C until required.…”

Section: Chemicalsmentioning

confidence: 99%

“…Differentiated PC12 cells were seeded into 6-well plate (2x10 5 cells/well) and grown in complete RPMI 1640 medium for 24 h at 37˚C. Cells were then pretreated with or without DHYZ (10, 20 and 40 µg/ml) for 1 h at 37˚C, after which the cells were exposed to 20 µM aggregated Aβ [25][26][27][28][29][30][31][32][33][34][35] for 24 h at 37˚C. Cells treated with the same volume of RPMI 1640 medium alone were used as a control.…”

Section: Measurement Of Mitochondrial Membrane Potential (Mmp)mentioning

confidence: 99%

“…DCFH-DA is cleaved intracellularly by nonspecific esterases to form DCFH, which is further oxidized by ROS to form the fluorescent compound DCF. Differentiated PC12 cells were pre-incubated with or without DHYZ and exposed to aggregated Aβ [25][26][27][28][29][30][31][32][33][34][35] , as described for the aforementioned MPP assay. Cells were then stained with DCFH-DA at 37˚C for 20 min in the dark.…”

Section: Measurement Of Reactive Oxygen Species (Ros) Generationmentioning

confidence: 99%

“…In the present study, the Cyt-C concentration in the cytosol was measured by ELISA. PC12 cells treated with Aβ [25][26][27][28][29][30][31][32][33][34][35] showed an increase in cytosolic Cyt-C levels compared with the control levels (P<0.01). Pretreatment with 10-40 µg/ml DHYZ significantly attenuated Aβ 25-35 -induced Cyt-C release in a dose-dependent manner (P<0.01), and the effect of 40 µg/ml DHYZ in reducing Cyt-C release was significantly greater than that observed for 10 µg/ml DHYZ (P<0.01; Fig.…”

Section: Effects Of Dhyz On Aβ-induced Cyt-c Release In Pc12 Cellsmentioning

confidence: 99%

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Di-Huang-Yi-Zhi herbal formula attenuates amyloid-β-induced neurotoxicity in PC12 cells

Chen

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Traditional Chinese medicine can be used for Alzheimer's disease management, such as the modern herbal formula Di-Huang-Yi-Zhi (DHYZ). In the present study, neuronal differentiated PC12 cells were used as a model to evaluate the effects of DHYZ against amyloid-β peptide 25-35 (Aβ 25-35 ) induced neurotoxicity, particularly regarding cell proliferation, apoptosis and related events. Following treatment with DHYZ, cell viability, cell membrane damage, apoptosis, mitochondrial membrane potential, cytochrome c release, caspase-3 activity and levels of reactive oxygen species in PC12 cells were detected. The results demonstrated that pretreatment with DHYZ significantly protected PC12 cells from Aβ 25-35 -induced proliferation inhibition, lactate dehydrogenase release and apoptosis, as well as upregulating mitochondrial membrane potential and downregulating cytochrome c release and caspase-3 activation. DHYZ also inhibited the Aβ 25-35 -induced reactive oxygen species generation in PC12 cells. These observations suggest that DHYZ protected PC12 cells from the Aβ-induced neurotoxicity.

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Section: Discussionmentioning

confidence: 99%

Section: Chemicalsmentioning

confidence: 99%

Section: Measurement Of Mitochondrial Membrane Potential (Mmp)mentioning

confidence: 99%

Section: Measurement Of Reactive Oxygen Species (Ros) Generationmentioning

confidence: 99%

Section: Effects Of Dhyz On Aβ-induced Cyt-c Release In Pc12 Cellsmentioning

confidence: 99%

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Di-Huang-Yi-Zhi herbal formula attenuates amyloid-β-induced neurotoxicity in PC12 cells

Chen

et al. 2017

Experimental and Therapeutic Medicine

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Advances in understanding of health‐promoting benefits of medicine and food homology using analysis of gut microbiota and metabolomics

et al. 2020

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The health-promoting benefits of medicine and food homology (MFH) are known for thousands of years in China. However, active compounds and biological mechanisms are unclear, greatly limiting clinical practice of MFH. The advent of gut microbiota analysis and metabolomics emerge as key tools to discover functional compounds, therapeutic targets, and mechanisms of benefits of MFH. Such studies hold great promise to promote and optimize functional efficacy and development of MFH-based products, for example, foods for daily dietary supplements or for special medical purposes. In this review, we summarized pharmacological effects of 109 species of MFH approved by the Health and Fitness Commission in 2015. Recent studies applying genome sequencing of gut microbiota and metabolomics to explain the activity of MFH in prevention and management of health consequences were extensively reviewed. We discussed the potentiality in future to decipher functional activities of MFH by applying metabolomics-based polypharmacokinetic strategy and multiomics technologies. The needs for personalized MFH recommendations and comprehensive databases have also been highlighted. This review emphasizes current achievements and challenges of the analysis of gut microbiota and metabolomics as a new avenue to understand MFH. K E Y W O R D S gut microbiota, medicine and food homology, metabolomics, multiomics technology Abbreviations: IBD, inflammatory bowel disease; LPS, lipopolysaccharide; MFH, medicine and food homology; SCFA, short-chain fatty acid; TCMIO, a comprehensive database of traditional Chinese medicine on immuno-oncology; WEGL, water extract of Ganoderma lucidum mycelium This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Assessing the effect of 5‐hydroxymethylfurfural on selected components of immune responses in mice immunised with ovalbumin

et al. 2017

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5-Hydroxymethylfurfural, an antioxidant agent from Alpinia oxyphylla Miq. improves cognitive impairment in Aβ1–42 mouse model of Alzheimer's disease

Cited by 75 publications

References 31 publications

Di-Huang-Yi-Zhi herbal formula attenuates amyloid-β-induced neurotoxicity in PC12 cells

Di-Huang-Yi-Zhi herbal formula attenuates amyloid-β-induced neurotoxicity in PC12 cells

Advances in understanding of health‐promoting benefits of medicine and food homology using analysis of gut microbiota and metabolomics

Assessing the effect of 5‐hydroxymethylfurfural on selected components of immune responses in mice immunised with ovalbumin

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