5-Hydroxytryptamine receptor subtypes participating in pulmonary edema in dogs

Sumita, Tatsuya; Ishikawa, Naohisa; Hashiba, Yukihiro; Takagi, Kenzo; Satake, Tatsuo

doi:10.1016/0014-2999(89)90232-x

Cited by 15 publications

(12 citation statements)

References 14 publications

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“…The absolute values of R t reported in canine lungs average 16.5 cmH20 min L -1 per 100 g with a range of 15.7-17.4 cmH20 rain L -1 per 100 g (Rippe et al, 1987;Sumita et al, 1989). On that basis, the partitioning of R t performed by Hakim (1988) allows easy calculation of absolute values for R , R ', R' and Rv, these being a la V respectively 6.1, 1.6, 2.6 and 6.1 cmH20 min L -1 per 100 g. In rabbits, the corresponding values are 8.33 + 1.78 (mean _+ SEM) (Rt); 3.10 _+ 0.981(Ra); 1.98 -+ R ° + 0.54 ( a); 0.96 _ 0.25 (Rv') and 2.29 _+ 0.50 (Rv) cmH20 min L-per 100 g, respectively (Delaunois et aL, 1991).…”

Section: Discussionmentioning

confidence: 98%

“…The high resistance located downstream of the capillaries in pigs might favour the development of capillary hypertension during vasoconstriction and consequently the passage of fluid through the endothelium. The mean value of the capillary filtration coefficient in dogs calculated from data reported by different workers averages 0.11 ml min -1 (cmH20) -1 per 100 g with a 1 1 range of 0.053-0.180 ml min-(cmHzO)-per 100 g (Erhart et al, 1984;Seeger et aL, 1986;Allison et al, 1988;Sumita et al, 1989). The values obtained in this study (0.193-0.202 ml min -1 (cmH20) -1 per 100 g) were two times greater than this mean value and no difference was observed between Landrace animals and Minipigs.…”

Section: Discussionmentioning

confidence: 98%

“…This value was divided by hP c and by the lung weight to give Kf c expressed in millilitres per minute per cmH~O per 100 g lung weight (the filtrate wa's assumed to have a density of 1.0). The detads of this method have been published previously (Drake and Gabel, 1981;Sumita et al, 1989). Tracings showing how the weight of the lungs changes when the Pc is increased are shown in Figure 3.…”

Section: Estimation Of the Capillary Filtration Coefficientmentioning

confidence: 99%

“…Nevertheless, few studies have been devoted to the distribution of blood flow resistance or to the microvascular permeability, which are two important factors in the development of pulmonary interstitial oedema. Isolated perfused lung preparations have been extensively used in dogs (Rippe et aL, 1987;Hakim, 1988;Sumita et al, 1989), guinea-pigs (Selig et al, 1986), lambs (Yoshimura et al, 1989), rabbits (Littner et aL, 1986;Seeger et al, 1987), rats (McMurty et al, 1978), ferrets and cats (Peake et al, 1981). In pigs, the hypertensive effects of hypoxia or of the heparin-protamine complex have been studied ex vivo (Mitzner and Sylvester, 1981;Sylvester et aL, 1983;Rock et al, 1985;Degges et al, 1987;Hakim and Malik, 1988).…”

Section: Introductionmentioning

confidence: 99%

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Permeability of the endothelium and partitioning of the pulmonary blood flow resistance in isolated perfused pig lungs: Effects of breed and age

et al. 1992

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The right and left lungs of 5 healthy Minipigs and of 13 healthy Landrace piglets were isolated, perfused at constant pressure and maintained in an isogravimetric state under zone III conditions (pulmonary venous pressure greater than alveolar pressure). By applying the double, arterial and venous, occlusion technique, the total blood flow resistance (R) was partitioned into four components: arterial (Ra), pre- (Ra') and post-capillary (Rv') and venous (Rv). The capillary filtration coefficient (Kf,c) was evaluated by measuring the weight gained by the lungs when the arterial and venous pressures were suddenly increased. In the youngest Landrace piglets (5 weeks old), there was an uncontrolled vasoconstriction which sometimes prevented perfusion of the lungs and induced a large increase in Rt. These high values of Rt were decreased by tolazoline administration. The values of Rt recorded in older pigs (12-13 weeks old) were lower in Minipigs (33.66 +/- 3.77 cmH2O min L-1 per 100 g of lungs; n = 5) than in Landrace piglets (55.20 +/- 6.18 cmH2O min L-1 per 100 g; n = 5). This breed difference was due to the differences in Ra' and Rv'. The mean values of Kf,c were 0.193 +/- 0.015 and 0.202 +/- 0.029 ml min (cmH2O)-1 per 100 g of the lungs in Minipigs and Landrace piglets respectively. All these parameters were stable for the 3 hours following the equilibrium period. It was concluded that: (1) There is an age-related maturation of the control of the vasomotor tone in porcine lungs. (2) Pulmonary microvascular haemodynamics are influenced by the breed of the pigs. (3) There was no difference in the Kf,c values between both the breeds. (4) A comparison of the values reported for dogs and rabbits with our data shows that the pre- and post-capillary resistances and, to a lesser extent, the arterial and venous resistances are relatively high in pigs.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Section: Estimation Of the Capillary Filtration Coefficientmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Permeability of the endothelium and partitioning of the pulmonary blood flow resistance in isolated perfused pig lungs: Effects of breed and age

et al. 1992

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“…This value was divided by ~Dc to give Kf, c expressed in millilitres (the fdtrate was assumed to have a density of 1.0) per minute per cmH20 per 100 g lung weight. Further details of this method have been published previously (Drake and Gabel, 1981;Sumita et aL, 1989;Gustin et al, 1992).…”

Section: Estimation Of Capillary Filtration Coefficientmentioning

confidence: 99%

Endotoxin-induced microvascular injury in isolated and perfused pig lungs

1992

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The lungs of 13 healthy Landrace piglets were isolated, perfused and maintained in an isogravimetric state under zone III conditions. By applying vascular occlusion methods, the total blood flow resistance (Rt) was partitioned into four components: arterial (Ra), pre- (Ra') and post-capillary (Rv'), and venous (Rv). The capillary filtration coefficient (Kfc) was evaluated using a gravimetric technique. A bolus of 55 micrograms of Escherichia coli endotoxins (LPS) per 100 g of lung was injected into the arterial reservoir of eight lungs, followed by an infusion of LPS at a rate of 55 micrograms per 100 g of lung per hour for 180 min. A bolus of theophylline (85 mg per 100 g of lung weight) was injected into the arterial reservoir after the last determination of the Kfc value. All the parameters were evaluated again when the lungs reached a new steady state. Endotoxin induced a significant increase in Rt from 54.7 +/- 7.0 at zero time to 184.7 +/- 44.2 cmH2O min L-1 (100 g)-1 180 minutes later, which can be ascribed to the increase in Ra' and Rv'. These haemodynamic modifications were related to the increases in the arterial pressure and in the pressure at the distal end of the arterial segment and to the decreases in the pressure at the proximal end of the venous segment and in the blood flow. The capillary pressure and the lung weight remained unchanged. Endotoxin infusion induced an increase in the Kfc value from 0.208 +/- 0.011 (at t = 0) to 0.391 +/- 0.034 ml min-1 (cmH2O)-1 (100 g)-1 (at t = 180). Administration of theophylline significantly reduced Rt,Ra,Ra' and Rv' towards or under the baseline values and also induced a significant increase in the lung weight and in the Kfc value. It was concluded that the endotoxin-induced increase in the total blood flow resistance can be ascribed to a vasospasm occurring at the level of the pre- and post-capillary small vessels and that changes in the permeability of the endothelium greatly contribute to the development of the pulmonary oedema observed in endotoxaemic pigs.

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