51V NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium

Žižić, Milan; Miladinović, Zoran; Stanić, Marina; Hadžibrahimović, Mirzeta; Živić, Miroslav; Zakrzewska, Joanna

doi:10.1016/j.resmic.2016.04.012

Cited by 11 publications

(6 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in our control experiments the AKT signal in HEPG2 cells has not been activated (Figure 5c), suggesting that AKT phosphorylation by MetV, MetfDeca and NaDeca can be attributed to the activation of PTB-1B by orthovanadate (V 1 ) [32]. On the other hand, for NaDeca compound, the tetramer species is formed at the same speed than V 1 (Table 1), V 4 could be the one that is reduced [55] and the vanadium (IV) species VO 2+ is activating the AKT pathway in a PI3K-dependent manner by ROS, like in the case of VOSO 4 that exhibited a 17-fold increase in the phosphorylation of AKT in HEPG2 cells at 25 µM concentration [56]. In 2015 Levina and coworkers performed a speciation study by XANES spectroscopy, where for 1 mM of orthovanadate in HEPG2 cells with DMEM medium after 24 h, 50% of the initial vanadium was found as tetrahedral species of V 5+ (V 1 , V 2 , V 4 and V 5 are tetrahedral), 30% as V 4+ moieties with a coordination number of six and 20% as V 4+ with a coordination number of five [19], this study supports our observation that after 24 h not more decavanadate species are present in solution.…”

Section: Proteins Expressionmentioning

confidence: 86%

Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity

et al. 2020

View full text Add to dashboard Cite

The kinetics of the decomposition of 0.5 and 1.0 mM sodium decavanadate (NaDeca) and metforminium decavanadate (MetfDeca) solutions were studied by 51V NMR in Dulbecco’s modified Eagle’s medium (DMEM) medium (pH 7.4) at 25 °C. The results showed that decomposition products are orthovanadate [H2VO4]− (V1) and metavanadate species like [H2V2O7]2− (V2), [V4O12]4− (V4) and [V5O15]5− (V5) for both compounds. The calculated half-life times of the decomposition reaction were 9 and 11 h for NaDeca and MetfDeca, respectively, at 1 mM concentration. The hydrolysis products that presented the highest rate constants were V1 and V4 for both compounds. Cytotoxic activity studies using non-tumorigenic HEK293 cell line and human liver cancer HEPG2 cells showed that decavanadates compounds exhibit selectivity action toward HEPG2 cells after 24 h. The effect of vanadium compounds (8–30 μM concentration) on the protein expression of AKT and AMPK were investigated in HEPG2 cell lines, showing that NaDeca and MetfDeca compounds exhibit a dose-dependence increase in phosphorylated AKT. Additionally, NaDeca at 30 µM concentration stimulated the glucose cell uptake moderately (62%) in 3T3-L1 adipocytes. Finally, an insulin release assay in βTC-6 cells (30 µM concentration) showed that sodium orthovanadate (MetV) and MetfDeca enhanced insulin release by 0.7 and 1-fold, respectively.

show abstract

Section: Proteins Expressionmentioning

confidence: 86%

Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Alternatively, these observations are consistent with a hydrophobic environment having been reported in some cases to alter composition and speciation and impact the shifting of observed signals. Combined, these studies showed convincingly that the cell wall is not responsible for reduction of vanadate (Hadzibrahimovic et al, 2017 ) and these studies also confirm that tetrameric vanadate is not able to enter the cells (Zizic et al, 2016 ) as reported in yeast as well (Crans et al, 2002 ) (Willsky et al, 1984a , b .)…”

Section: Discussionmentioning

confidence: 58%

“…These studies were very elegant and truly important, because they documented the formation of decavanadate in cells from monomeric vanadate for the first time (Willsky et al, 1984b ). Recently, Zakrzewska and Zivic reported a series of studies in fungi exploring the uptake of both vanadate and V(IV) using 51 V and EPR spectroscopy, as well as polarographic studies (Zizic et al, 2013 , 2016 ; Hadzibrahimovic et al, 2017 ). The initial 51 V NMR studies on Phycomyce blakesleeanus mycelium were supplemented with polarography, which allowed for the assignment of the signal at −535 ppm accumulating inside the fungi cells to V 1 even though the pH of the system was defined by HEPES buffer at 7.2 where V 1 generally has a peak position at a higher frequency (Zizic et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates

et al. 2018

View full text Add to dashboard Cite

51V NMR spectroscopy is used to document, using speciation analysis, that one oxometalate is a more potent growth inhibitor of two Mycobacterial strains than other oxovanadates, thus demonstrating selectivity in its interaction with cells. Historically, oxometalates have had many applications in biological and medical studies, including study of the phase-problem in X-ray crystallography of the ribosome. The effect of different vanadate salts on the growth of Mycobacterium smegmatis (M. smeg) and Mycobacterium tuberculosis (M. tb) was investigated, and speciation was found to be critical for the observed growth inhibition. Specifically, the large orange-colored sodium decavanadate (V10O286-) anion was found to be a stronger inhibitor of growth of two mycobacterial species than the colorless oxovanadate prepared from sodium metavanadate. The vanadium(V) speciation in the growth media and conversion among species under growth conditions was monitored using 51V NMR spectroscopy and speciation calculations. The findings presented in this work is particularly important in considering the many applications of polyoxometalates in biological and medical studies, such as the investigation of the phase-problem in X-ray crystallography for the ribosome. The findings presented in this work investigate the interactions of oxometalates with other biological systems.

show abstract

“…All spectra were recorded with an Apollo spectrometer (Tecmag, USA). Detailed experimental conditions were explained in our previous paper [6].…”

Section: Nuclear Magnetic Resonance (Nmr) Spectroscopymentioning

confidence: 99%

“…Determination of the relative content of phosphorus compounds in fungal cells provides key information about cell energy status. According to our knowledge 31 P NMR spectrum was assessed for the first time for C. truncorum species, and assignments were done according to Žižić et al [6]. The major peaks in the spectrum with chemical shifts of -22.1 ppm, -12 ppm, -10.2 ppm, and 1.3 ppm was assigned to core-polyphosphates (PPc), uridine diphosphate glucose (UDPG), nicotinamide adenine dinucleotide phosphate (NADP(H)+UDPG) and inorganic phosphate (Pi), respectively (Fig.…”

Section: Nmrmentioning

confidence: 99%

Influence of Vanadium on the Growth and Metabolism of Coprinellus Truncorum Fungal Mycelium

Žižić¹,

Živić²,

Atlagić³

et al. 2021

Book of Proceedings: 1st International Conference on Chemo and BioInformatics

View full text Add to dashboard Cite

Fungi could absorb heavy metals, metalloids, or radionuclides, thus fungal species possess great potential in bioremediation. Since fungi absorb the vanadium, in the present study ability of Coprinellus truncorum mycelia for vanadate uptake and its intracellular metabolism were investigated. The submerged cultivated C. truncorum was exposed to a rising concentration of vanadate. 31P NMR spectroscopy was used to investigate phosphate metabolism of the mycelium, while the status of vanadium in the cell was followed by 51V NMR spectroscopy. The mycelium could grow, and overcome vanadate presence, up to the concentration of 1.6 mM in the submerged medium. 31P NMR measurements pointed out that vanadate induced changes in the concentration of the crucial metabolite containing phosphorus, particularly sugar phosphates. The major result of vanadate action is evinced through an appearance of a signal positioned at around 2.8 ppm, and an increased signal of hexose- phosphates. Using 51V NMR spectroscopy the presence of vanadate monomer in the mycelia of the fungal cell was confirmed.

show abstract

51V NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium

Cited by 11 publications

References 37 publications

Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity

Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity

Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates

Influence of Vanadium on the Growth and Metabolism of Coprinellus Truncorum Fungal Mycelium

Contact Info

Product

Resources

About