52. Changes in intracellular potassium and sodium content of 2-cell mouse embryos induced by exposure to vitrification concentrations of EG and Me2SO

Pogorelov, A. G.; Katkov, I.; Smolyaninova, E. I.; Gol’dshtein, D. V.; Isachenko, Vladimir; Isachenko, Evgenia

doi:10.1016/j.cryobiol.2006.10.053

Cited by 8 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Methods of the preparation making based on snap cryofi xation of the biological tissue were described previously [2,[12][13][14]16]. The initial step is cryofi xation of the object in liquid propane (-188 o C).…”

Section: Methodsmentioning

confidence: 99%

Analysis of Changes in Cell Volume of a Mouse Early Embryo Exposed to Osmotic Shock

et al. 2009

View full text Add to dashboard Cite

The impact of the osmotic component of the incubation medium for the volume of mouse early embryonic cell was studied by laser scanning microscopy. Common Dulbecco's medium caused a prolonged hyperosmotic effect. Adaptive phase of regulatory compensation for the osmotic shock was observed under hypotonic conditions. From these data, water permeability of the blastomer membrane is evaluated as 0.4 micro/(minxatm).

show abstract

Section: Methodsmentioning

confidence: 99%

Analysis of Changes in Cell Volume of a Mouse Early Embryo Exposed to Osmotic Shock

et al. 2009

View full text Add to dashboard Cite

show abstract

“…An important advantage of the use of nylon loop over conventional vitrification procedures is that the open system lacks any thermo-insulation layer; this, coupled with the small volume (< 1 µl) of vitrification solution, results in both rapid and uniform heat exchange during cooling (Lane et al, 1999a, b;Reed et al, 2002;Cai et al, 2005;Sheehan et al, 2006;Mukaida and Takahashi, 2008). By decreasing the volume of the cryoprotective solution we are able to increase dramatically the freezing speed and decrease the toxicity and osmotic side effects of the CPA (Pogorelov et al, 2006;Katkov and Pogorelov, 2007;Pogorelov et al, 2007). The rapid cooling achieved by the nylon loop carrier system prevents chilling injury and reduces the time of exposure to the CPA.…”

Section: Acta Veterinaria Hungarica 57 2009mentioning

confidence: 99%

Vitrification of cleavage stage mouse embryos by the cryoloop procedure

Klambauer

Keresztes

Kanyó³

et al. 2009

Acta Veterinaria Hungarica

View full text Add to dashboard Cite

By decreasing the volume of the cryoprotective solution it is possible to increase dramatically the freezing speed and -at the same time -reduce the toxicity and osmotic side effects of cryoprotectants (CPA). The objective of our study was to vitrify Day-3 cleavage stage mouse embryos (n = 229) with the cryoloop technology using a new composition of vitrification media. Embryos were exposed to a 2-step loading of CPA, ethylene glycol (EG) and propylene glycol (PG), before being placed on the surface of a thin filmy layer formed from the vitrification solution in a small nylon loop, then they were rapidly submerged into liquid nitrogen. After warming, the CPA was diluted out from the embryos by a 3-step procedure. Survival of embryos was based on morphological appearance after thawing and continued development to expanded blastocysts upon subsequent 48-hour culture. Embryos of the two control groups were either treated likewise except that they were not vitrified, or cultured in vitro without any treatment. Our data show that a high percentage of embryos survived (92.7%) vitrification in the mixture of EG and PG combined with cryoloop carrier and developed normally (89.1%) in vitro after thawing. To our knowledge this is the first report of the successful vitrification of cleavage stage mouse embryos using VitroLoop vitrification procedure.

show abstract

“…Among five common permeating cryoprotecting agents to be tested, toxicity was determined to be dimethylformamide> erythritol > DMSO > glycerol and ethylene glycol on mouse morulae (Kasai et al, 1981). By electron probe microanalysis, Pogorelov et al (2006Pogorelov et al ( & 2007a detected a dramatic decrease in intracellular potassium and sodium content in two-cell embryos treated with procedures mimicking vitrification in ethylene glycol, demonstrating a potential stress exerted on the embryos. When the mouse morulae were stored in 1.5M ethylene glycol or glycerol for 6 hours, the majority (>75%) of the embryos retained the capacity to develop into expanded blastocysts (Kasai et al, 1981).…”

Section: Cryoprotectant Toxicity To Mammalian Embryosmentioning

confidence: 99%